Click here to switch to the map view.

The map label for this gene is cmlA [H]

Identifier: 150397717

GI number: 150397717

Start: 2616683

End: 2617882

Strand: Direct

Name: cmlA [H]

Synonym: Smed_2519

Alternate gene names: 150397717

Gene position: 2616683-2617882 (Clockwise)

Preceding gene: 150397716

Following gene: 150397718

Centisome position: 69.19

GC content: 63.92

Gene sequence:

>1200_bases
ATGTCTTCAAAGCCACATGTCTGGCGCTATTCGCTGCCGGCAGCGCTGCTGCTGATGGCGCCCTTCGATATCCTCGCCTC
CCTGGCCATGGATATCTATCTCCCCATCGTTCCGGCGATGCCGGGCATTCTCGGGACCAGCCCCGCCGTGGTGCAGCTCA
CGCTGAGCCTCTATGTGGTCATGCTCGGCCTCGGCCAGATCGTCTTCGGGCCTCTCTCCGATCGCATCGGACGACGGCCC
GTGCTCATCGGCGGAGCGATGCTTTTCGCCGCGGCTTCGTTCTGCCTTGCGGCATCATCCAGCGCCATTCCTTTCGTCGC
CTTCCGCTTCCTGCAGGCGATTGGCGCATCTGCGGCCCTGGTGGCCACCTTCTCGACTATTCGGGATGTATATGGCAATC
GCCCTGAAAGCGTGGTCATCTACAGCCTGTTCAGTTCCATCCTGGCATTCGTGCCGGCTCTGGGGCCGATCGCCGGTGCC
GCGCTGGCGGAGAGGTTCGGATGGCGCTCGATCTTCGTGACACTGGGCGCATTTGCCGTAATCGCGCTGCTGCAGGCCTT
GCCCCGGTGGCATGAATCTCGCCCTTCGGCGCCCGCGCCGTCGCGGCACGCATTCGGGCCGCTTCTCCGCAGCCTCGCCT
TCTGGACCTATACTCTCGGCTTCAGCGCCGCCATGGGCACCTTCTTCGTGTTCTTTTCCACCTCACCGCGCGTGCTGATC
GGCAAGGCAGGATATTCGGAGCTTCAGTTCAGCCTCGCCTTTGCCACCGTCGCGGCCGTCATGATCTTCACGGCGCGCTT
TGCCAAGCAGTTTGTCTTCCGATGGGGAACTTCGGGCAGCCTCCTGCGGGGCATGTGCATGTTGCTCCTCGGGGCGGCAC
TCCTGGCTGCCGGGCAAGGCCTCGGCACCCCTTCGTCCTGGGTCTTCATCGCGCCCATGTGGGTCATGGCCGTCGGCATC
GTCTTCACGGTGTCGGTAACCGCGAACGGCGCTCTCAAGGATTTTGGAGACATGGCGGGTTCGGCGGTCGCAGTCTATTT
CTGCGTCCAAAGCCTCATCGTCGCGACGGCGGGGACACTGCTCGTGATCGCACTGCCCGGCGACACCGCATGGCCGCTTG
CGGCCTATGCCTCGATCATGGCCGGTATCGTGCTGATCGCGCGCCGGCAGCTGCATTCCGGAAGGCAGCCGCAGACATGA

Upstream 100 bases:

>100_bases
CAACAACGCATTTCCTGTTGTTGCGGCGTCTGTCGATCCGACCCTGATCCTGATAGGCCGACCGCCGCCGAATGTTCCTC
ATTTGAGCGGAAGTCTTTCC

Downstream 100 bases:

>100_bases
TTGTAGAGGAAGTCGGCAAAAGCCCGGCTTCCTCACCCGCTAAACGAGGGTGCTGAACCCTTTGTTAACGCTGATCTGGC
AGAAGTGAAGCCCTGTATTG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSSKPHVWRYSLPAALLLMAPFDILASLAMDIYLPIVPAMPGILGTSPAVVQLTLSLYVVMLGLGQIVFGPLSDRIGRRP
VLIGGAMLFAAASFCLAASSSAIPFVAFRFLQAIGASAALVATFSTIRDVYGNRPESVVIYSLFSSILAFVPALGPIAGA
ALAERFGWRSIFVTLGAFAVIALLQALPRWHESRPSAPAPSRHAFGPLLRSLAFWTYTLGFSAAMGTFFVFFSTSPRVLI
GKAGYSELQFSLAFATVAAVMIFTARFAKQFVFRWGTSGSLLRGMCMLLLGAALLAAGQGLGTPSSWVFIAPMWVMAVGI
VFTVSVTANGALKDFGDMAGSAVAVYFCVQSLIVATAGTLLVIALPGDTAWPLAAYASIMAGIVLIARRQLHSGRQPQT

Sequences:

>Translated_399_residues
MSSKPHVWRYSLPAALLLMAPFDILASLAMDIYLPIVPAMPGILGTSPAVVQLTLSLYVVMLGLGQIVFGPLSDRIGRRP
VLIGGAMLFAAASFCLAASSSAIPFVAFRFLQAIGASAALVATFSTIRDVYGNRPESVVIYSLFSSILAFVPALGPIAGA
ALAERFGWRSIFVTLGAFAVIALLQALPRWHESRPSAPAPSRHAFGPLLRSLAFWTYTLGFSAAMGTFFVFFSTSPRVLI
GKAGYSELQFSLAFATVAAVMIFTARFAKQFVFRWGTSGSLLRGMCMLLLGAALLAAGQGLGTPSSWVFIAPMWVMAVGI
VFTVSVTANGALKDFGDMAGSAVAVYFCVQSLIVATAGTLLVIALPGDTAWPLAAYASIMAGIVLIARRQLHSGRQPQT
>Mature_398_residues
SSKPHVWRYSLPAALLLMAPFDILASLAMDIYLPIVPAMPGILGTSPAVVQLTLSLYVVMLGLGQIVFGPLSDRIGRRPV
LIGGAMLFAAASFCLAASSSAIPFVAFRFLQAIGASAALVATFSTIRDVYGNRPESVVIYSLFSSILAFVPALGPIAGAA
LAERFGWRSIFVTLGAFAVIALLQALPRWHESRPSAPAPSRHAFGPLLRSLAFWTYTLGFSAAMGTFFVFFSTSPRVLIG
KAGYSELQFSLAFATVAAVMIFTARFAKQFVFRWGTSGSLLRGMCMLLLGAALLAAGQGLGTPSSWVFIAPMWVMAVGIV
FTVSVTANGALKDFGDMAGSAVAVYFCVQSLIVATAGTLLVIALPGDTAWPLAAYASIMAGIVLIARRQLHSGRQPQT

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI48994889, Length=324, Percent_Identity=29.320987654321, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790146, Length=246, Percent_Identity=31.3008130081301, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788509, Length=395, Percent_Identity=27.3417721518987, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790794, Length=328, Percent_Identity=27.4390243902439, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082312, Length=331, Percent_Identity=24.773413897281, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787065, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42132; Mature: 42001

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKPHVWRYSLPAALLLMAPFDILASLAMDIYLPIVPAMPGILGTSPAVVQLTLSLYVV
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
MLGLGQIVFGPLSDRIGRRPVLIGGAMLFAAASFCLAASSSAIPFVAFRFLQAIGASAAL
HHHHHHHHCCCHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
VATFSTIRDVYGNRPESVVIYSLFSSILAFVPALGPIAGAALAERFGWRSIFVTLGAFAV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
IALLQALPRWHESRPSAPAPSRHAFGPLLRSLAFWTYTLGFSAAMGTFFVFFSTSPRVLI
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEE
GKAGYSELQFSLAFATVAAVMIFTARFAKQFVFRWGTSGSLLRGMCMLLLGAALLAAGQG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
LGTPSSWVFIAPMWVMAVGIVFTVSVTANGALKDFGDMAGSAVAVYFCVQSLIVATAGTL
CCCCCCCEEHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LVIALPGDTAWPLAAYASIMAGIVLIARRQLHSGRQPQT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSKPHVWRYSLPAALLLMAPFDILASLAMDIYLPIVPAMPGILGTSPAVVQLTLSLYVV
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
MLGLGQIVFGPLSDRIGRRPVLIGGAMLFAAASFCLAASSSAIPFVAFRFLQAIGASAAL
HHHHHHHHCCCHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
VATFSTIRDVYGNRPESVVIYSLFSSILAFVPALGPIAGAALAERFGWRSIFVTLGAFAV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
IALLQALPRWHESRPSAPAPSRHAFGPLLRSLAFWTYTLGFSAAMGTFFVFFSTSPRVLI
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEE
GKAGYSELQFSLAFATVAAVMIFTARFAKQFVFRWGTSGSLLRGMCMLLLGAALLAAGQG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
LGTPSSWVFIAPMWVMAVGIVFTVSVTANGALKDFGDMAGSAVAVYFCVQSLIVATAGTL
CCCCCCCEEHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LVIALPGDTAWPLAAYASIMAGIVLIARRQLHSGRQPQT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]