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The map label for this gene is rbsB [H]

Identifier: 150397675

GI number: 150397675

Start: 2557375

End: 2558340

Strand: Reverse

Name: rbsB [H]

Synonym: Smed_2475

Alternate gene names: 150397675

Gene position: 2558340-2557375 (Counterclockwise)

Preceding gene: 150397676

Following gene: 150397674

Centisome position: 67.65

GC content: 62.73

Gene sequence:

>966_bases
ATGTCCCGCATCAAAACACTTTTGGCAGGGTCCGTCGCCCTGTTCTGCCTGACCTCTGCCGCCATGGCCGAGGACTGCAA
GGTGGGCCTTACCATGCCCTCGCTGAACGCCCCCTATTTCGCCGCGCAGGTCGCTGCCGTCGAGAAGGCCGCGAAGGACA
AGGGATGCGAAATCACCACGGCCGACAGCCAGAATGACTTCTCCAAGCAGATCAACGATGTCGAGGATATGATCGCCAAG
GGGATCGACATCCTCATACTCAACCCGCGTGACCAGGAGGCGCTCGTTCCGGTAGCCAACGCGGCAACCGCGGCCGGCGT
GAAGGTCGTCGTGATGGACTCCACGCTGAATCCGAAGGCCAACGCCGTCACCCAGGTCCGTTCGTCGAACGATGAGAACG
GCCGCCTCGTCGGCGACTGGATCGCCAAGAAGATGAAGGGCAAGCCGATCAAGATGCTGCTTCTCTCGGGCAATCAGGGC
AATCCCGGTGGTCTCGAGCGCCGCATGGGCGTCATCAAGGGTATCGTCGAGGGCCAACTCGTCAACGAAGGCGCCGTCAA
CCTGCAGATTCTCGGCCAGGGCTGGGGGGATTGGTCGACGGATGGGGGCCTGAAGGCCGCCGAAGACCTGTTCCAGGCCC
ATCCGGATGCCAACCTCGTCGTCGGCGAGAACGACTCCATGGTTCTTGGCGCGGTACAGGCAGCCAAGGCCGCCGGCCTC
GAGGGCATCCTGTTCGCTGCGGCGGCCGACGGTCAGCGCGAGGCGCTCGAGATGATCAAGGAAGGGAGCTACGGCGCGAC
GGGCCTGAACGATCCGGACCTGGTCGGGCGCACCGCCTTCGACATCGGCTATAAGGCTTTCAAGGGCGAGCTTCCGGAGG
ACTTTCCGAAGCTCTACCTGACGCCGGCGGACGTCATCGCTCAGGAAAACGTGGACAAATACCACAACCCGCAATCAGTC
TTCTGA

Upstream 100 bases:

>100_bases
ATGGAGGATACACGCTCTGGTGACCGTATCCCGCACCGGGTGCGCACTTCGTCGTGTTTTCCGAACCTAAGAAAAGCAGT
CAATAAAAGGAGGAGTTGCA

Downstream 100 bases:

>100_bases
CCATATCCGTTTTCCGCTCACATCGAACAAAGGATGCACCGGTTTTCCGGTGCATTCTCTGGAACCCCAATGACCGAACT
TGTCAGCCTATCCGGTATCC

Product: monosaccharide-transporting ATPase

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSRIKTLLAGSVALFCLTSAAMAEDCKVGLTMPSLNAPYFAAQVAAVEKAAKDKGCEITTADSQNDFSKQINDVEDMIAK
GIDILILNPRDQEALVPVANAATAAGVKVVVMDSTLNPKANAVTQVRSSNDENGRLVGDWIAKKMKGKPIKMLLLSGNQG
NPGGLERRMGVIKGIVEGQLVNEGAVNLQILGQGWGDWSTDGGLKAAEDLFQAHPDANLVVGENDSMVLGAVQAAKAAGL
EGILFAAAADGQREALEMIKEGSYGATGLNDPDLVGRTAFDIGYKAFKGELPEDFPKLYLTPADVIAQENVDKYHNPQSV
F

Sequences:

>Translated_321_residues
MSRIKTLLAGSVALFCLTSAAMAEDCKVGLTMPSLNAPYFAAQVAAVEKAAKDKGCEITTADSQNDFSKQINDVEDMIAK
GIDILILNPRDQEALVPVANAATAAGVKVVVMDSTLNPKANAVTQVRSSNDENGRLVGDWIAKKMKGKPIKMLLLSGNQG
NPGGLERRMGVIKGIVEGQLVNEGAVNLQILGQGWGDWSTDGGLKAAEDLFQAHPDANLVVGENDSMVLGAVQAAKAAGL
EGILFAAAADGQREALEMIKEGSYGATGLNDPDLVGRTAFDIGYKAFKGELPEDFPKLYLTPADVIAQENVDKYHNPQSV
F
>Mature_320_residues
SRIKTLLAGSVALFCLTSAAMAEDCKVGLTMPSLNAPYFAAQVAAVEKAAKDKGCEITTADSQNDFSKQINDVEDMIAKG
IDILILNPRDQEALVPVANAATAAGVKVVVMDSTLNPKANAVTQVRSSNDENGRLVGDWIAKKMKGKPIKMLLLSGNQGN
PGGLERRMGVIKGIVEGQLVNEGAVNLQILGQGWGDWSTDGGLKAAEDLFQAHPDANLVVGENDSMVLGAVQAAKAAGLE
GILFAAAADGQREALEMIKEGSYGATGLNDPDLVGRTAFDIGYKAFKGELPEDFPKLYLTPADVIAQENVDKYHNPQSVF

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=30.5841924398625, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1790674, Length=301, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788473, Length=301, Percent_Identity=28.9036544850498, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789990, Length=308, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790526, Length=291, Percent_Identity=24.3986254295533, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33861; Mature: 33730

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKTLLAGSVALFCLTSAAMAEDCKVGLTMPSLNAPYFAAQVAAVEKAAKDKGCEITT
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEE
ADSQNDFSKQINDVEDMIAKGIDILILNPRDQEALVPVANAATAAGVKVVVMDSTLNPKA
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCCH
NAVTQVRSSNDENGRLVGDWIAKKMKGKPIKMLLLSGNQGNPGGLERRMGVIKGIVEGQL
HHHHHHHCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEE
VNEGAVNLQILGQGWGDWSTDGGLKAAEDLFQAHPDANLVVGENDSMVLGAVQAAKAAGL
ECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHCCC
EGILFAAAADGQREALEMIKEGSYGATGLNDPDLVGRTAFDIGYKAFKGELPEDFPKLYL
CEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHCHHHHCCCCCCCCHHEEE
TPADVIAQENVDKYHNPQSVF
CHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
SRIKTLLAGSVALFCLTSAAMAEDCKVGLTMPSLNAPYFAAQVAAVEKAAKDKGCEITT
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEE
ADSQNDFSKQINDVEDMIAKGIDILILNPRDQEALVPVANAATAAGVKVVVMDSTLNPKA
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCCH
NAVTQVRSSNDENGRLVGDWIAKKMKGKPIKMLLLSGNQGNPGGLERRMGVIKGIVEGQL
HHHHHHHCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCEE
VNEGAVNLQILGQGWGDWSTDGGLKAAEDLFQAHPDANLVVGENDSMVLGAVQAAKAAGL
ECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHCCC
EGILFAAAADGQREALEMIKEGSYGATGLNDPDLVGRTAFDIGYKAFKGELPEDFPKLYL
CEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHCHHHHCCCCCCCCHHEEE
TPADVIAQENVDKYHNPQSVF
CHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]