Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 150397611

GI number: 150397611

Start: 2487036

End: 2488052

Strand: Direct

Name: gcvA [H]

Synonym: Smed_2411

Alternate gene names: 150397611

Gene position: 2487036-2488052 (Clockwise)

Preceding gene: 150397602

Following gene: 150397612

Centisome position: 65.76

GC content: 65.0

Gene sequence:

>1017_bases
TTGTCGAGCCGCCTGCCCCCGCTGAACCCGCTTCGCGCCTTCGAGGCAACGGCCCGGCGCGGCTCCGTTTCCGCCGCGGC
GCGTGAGCTCAACGTCACCCACGGGGCCGTCAGTCACCAGATCCGCGCGCTCGAAGCTTCCTTCAACGCGCCCCTTTTCG
AGCGTGGGGGCAAGAGATTGAAGCTGACGCCGCAGGGTGCATTGCTTCTGCCGGCGGTCACGCATGCCTTCGCCGAAATC
GCAGCCGCGACAGCGGCGATGACGCGGCCTGCGACCAGCGGCGAGCTTACCGTCACCTGCGTCCCGGCATTGCTCTCCTT
CTGGATGATCCCCCGTCTGCACCTCTTCACCGAGCAGTTCCCCGATGTCCAGTTGAGGCTGATCGCCTCCAATGACGAGG
CTCACCTCGATTCCCCCGACGTCGATGTCTGCCTTCTCTATGGGGACGGCAACTGGCAGGATTGCTGGGCAAGGCTCTGG
AGCCGGCTCGCGCTTTTTCCGGTCGTCAGCCCGACGCTTCTCAATATGAGACCGCTGCGCTCCATCCGGGACTTGCGGGA
CCACGTGATGCTGCATGGCGACGACGGCCGCGAATGGAACACCTGGCTCGCTGCAGCCGACGCGACCGACCTGCAGCGCG
GCCGCCAGCATTTCATGAGCGATGCACGCCTTTCGACCGAGGCGGCGCTCCACAATCAGGGCGTTGCGCTCGGCGACACA
ATCACCGCCGGCAGCCTGATCGCCCGCGGCGAGTTGATCGCCCCCTTCGACCTGACGGTTCCGGCCAATGACGCCTTCTT
CATTGCCTGCCGCAACGAGGTGCGCGCCGCGCCGATCGTGCGCGTTTTCATCGACTGGCTTTTTGCCGCACTCGAAAGCG
ACCCAATGCCGGAGTTGCAGATATCCGCCCGCACGGTTATCCGCAGCCGTGTACCCAAGCCGGAGAGTATTCAACACGTT
TCCTCGCCGCGCAGCCAGCGCATCGAACGGCCGCAAAAGCGCAGGTTGAAGTCATGA

Upstream 100 bases:

>100_bases
GCATATTAGGAGACAAATCTTCTGGAAAATCAATTGACATTGTTATAGCATTTAGTGTGAGAAAAATTCACATGCTCATC
GCCTCTCCCGGAGTTGTTTC

Downstream 100 bases:

>100_bases
TACCGGACGGGCGACGACCCTCGCGCCCCCGCACATTACCAGCAACAGGAAATCCGCCATGCCGCATTTCAATCCCCTTG
TCGAAAAGCTCTCGCCACCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSRLPPLNPLRAFEATARRGSVSAAARELNVTHGAVSHQIRALEASFNAPLFERGGKRLKLTPQGALLLPAVTHAFAEI
AAATAAMTRPATSGELTVTCVPALLSFWMIPRLHLFTEQFPDVQLRLIASNDEAHLDSPDVDVCLLYGDGNWQDCWARLW
SRLALFPVVSPTLLNMRPLRSIRDLRDHVMLHGDDGREWNTWLAAADATDLQRGRQHFMSDARLSTEAALHNQGVALGDT
ITAGSLIARGELIAPFDLTVPANDAFFIACRNEVRAAPIVRVFIDWLFAALESDPMPELQISARTVIRSRVPKPESIQHV
SSPRSQRIERPQKRRLKS

Sequences:

>Translated_338_residues
MSSRLPPLNPLRAFEATARRGSVSAAARELNVTHGAVSHQIRALEASFNAPLFERGGKRLKLTPQGALLLPAVTHAFAEI
AAATAAMTRPATSGELTVTCVPALLSFWMIPRLHLFTEQFPDVQLRLIASNDEAHLDSPDVDVCLLYGDGNWQDCWARLW
SRLALFPVVSPTLLNMRPLRSIRDLRDHVMLHGDDGREWNTWLAAADATDLQRGRQHFMSDARLSTEAALHNQGVALGDT
ITAGSLIARGELIAPFDLTVPANDAFFIACRNEVRAAPIVRVFIDWLFAALESDPMPELQISARTVIRSRVPKPESIQHV
SSPRSQRIERPQKRRLKS
>Mature_337_residues
SSRLPPLNPLRAFEATARRGSVSAAARELNVTHGAVSHQIRALEASFNAPLFERGGKRLKLTPQGALLLPAVTHAFAEIA
AATAAMTRPATSGELTVTCVPALLSFWMIPRLHLFTEQFPDVQLRLIASNDEAHLDSPDVDVCLLYGDGNWQDCWARLWS
RLALFPVVSPTLLNMRPLRSIRDLRDHVMLHGDDGREWNTWLAAADATDLQRGRQHFMSDARLSTEAALHNQGVALGDTI
TAGSLIARGELIAPFDLTVPANDAFFIACRNEVRAAPIVRVFIDWLFAALESDPMPELQISARTVIRSRVPKPESIQHVS
SPRSQRIERPQKRRLKS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=34.4827586206897, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.0276816608997, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=31.3725490196078, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=27.0547945205479, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=122, Percent_Identity=33.6065573770492, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37420; Mature: 37289

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRLPPLNPLRAFEATARRGSVSAAARELNVTHGAVSHQIRALEASFNAPLFERGGKRL
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCEE
KLTPQGALLLPAVTHAFAEIAAATAAMTRPATSGELTVTCVPALLSFWMIPRLHLFTEQF
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
PDVQLRLIASNDEAHLDSPDVDVCLLYGDGNWQDCWARLWSRLALFPVVSPTLLNMRPLR
CCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCHHHHH
SIRDLRDHVMLHGDDGREWNTWLAAADATDLQRGRQHFMSDARLSTEAALHNQGVALGDT
HHHHHHHHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ITAGSLIARGELIAPFDLTVPANDAFFIACRNEVRAAPIVRVFIDWLFAALESDPMPELQ
CCCCCHHCCCCEECCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
ISARTVIRSRVPKPESIQHVSSPRSQRIERPQKRRLKS
HHHHHHHHHCCCCCHHHHHHCCCHHHHHCCHHHHCCCC
>Mature Secondary Structure 
SSRLPPLNPLRAFEATARRGSVSAAARELNVTHGAVSHQIRALEASFNAPLFERGGKRL
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCEE
KLTPQGALLLPAVTHAFAEIAAATAAMTRPATSGELTVTCVPALLSFWMIPRLHLFTEQF
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
PDVQLRLIASNDEAHLDSPDVDVCLLYGDGNWQDCWARLWSRLALFPVVSPTLLNMRPLR
CCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCHHHHH
SIRDLRDHVMLHGDDGREWNTWLAAADATDLQRGRQHFMSDARLSTEAALHNQGVALGDT
HHHHHHHHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ITAGSLIARGELIAPFDLTVPANDAFFIACRNEVRAAPIVRVFIDWLFAALESDPMPELQ
CCCCCHHCCCCEECCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
ISARTVIRSRVPKPESIQHVSSPRSQRIERPQKRRLKS
HHHHHHHHHCCCCCHHHHHHCCCHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]