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The map label for this gene is potB [H]

Identifier: 150397607

GI number: 150397607

Start: 2482441

End: 2483295

Strand: Reverse

Name: potB [H]

Synonym: Smed_2407

Alternate gene names: 150397607

Gene position: 2483295-2482441 (Counterclockwise)

Preceding gene: 150397608

Following gene: 150397606

Centisome position: 65.66

GC content: 62.34

Gene sequence:

>855_bases
ATGAGACGCGAACCGCCCCGGACCCTCGCCGACTATCTGCCGCTTTTCTTCCCCGCGGGCATGCTCACGATTTTCTTCGT
GGTACCCTTCGGCACGATGATCGCCGTCAGTTTCTTTCAGCGCCAGCAGGGCGGCTTCTATACGCCGGCCTTCGTTTACG
ACAATTATGCCCGCTTCCTCTCGGCCTTCTTCGGCGGTGTGCTTGGCTTTTCGCTGATGTTGGCGATCACGGTCGCCCTG
TGCTGCGTCGTGTTGGCGCTGCCCTTCACCTATCTGCTCACGCGCATGTCGCGCCGGGTCCAGGTGGTCTGGCTCGTGGC
TCTGCTCTCCGTGCTTTCTCTCTCCGAAGTCATTATCGGCTTTGCGTGGTCGACCCTTTTCTCGCGCACTGCCGGCATCA
CCAACATCCTTGTTGCGCTCGGGCTCATGGGTGAGGCGAAGGCGCTTACTCCGAGCTTCGCCGCTGTGCTGACGGGCATG
GTCTATCAAGCATTTCCCTACACGGTGCTCGTGCTTTTCCCTGCGCTCGTGCGCCTTGATCCGACGCTGACCGAAGCGGC
ACGCACGCTCGGCGCGTCCCCGCTCAGGGCGTTCTTCACCGTGGTGGTTCCAGCGCTGCGGAACACGATCACGGCCACGC
TGATCATGGTCTTCATCTTCGCGCTCGGTTCCTATCTCTTGCCGCAGCTGCTCGGGCGGCCGCAGCACTGGACGCTCTCG
GTGCTCATCACCGATCAGGCAATCTACCAGTCCAACATGCCGTTCGCCGCGGCGATGGCAGTGTTCCTCGTGCTGGTGAC
GCTGGGGCTGGTGGCGCTTACGGTGCTTGCCGGACGAAGGGGAGAAGCGGCATGA

Upstream 100 bases:

>100_bases
AGATCGTCGCCGTCTCCACGCCGCGTGCGCGGCCGGAGGTCACGGTAGGGCAGCAGGTCGGCATTGGTCTCACGCCTGAT
GTTTGCGTGGTGCTCGCCAA

Downstream 100 bases:

>100_bases
CGTCTGCTTTCCGCACGCTCTATTTCTTCCTGATCGGCCTTTTTCTCGCGCTGCCGCTGATCGTCGTTGCCGGTGTCTCG
GTCAATGCGAAGCAGACGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRREPPRTLADYLPLFFPAGMLTIFFVVPFGTMIAVSFFQRQQGGFYTPAFVYDNYARFLSAFFGGVLGFSLMLAITVAL
CCVVLALPFTYLLTRMSRRVQVVWLVALLSVLSLSEVIIGFAWSTLFSRTAGITNILVALGLMGEAKALTPSFAAVLTGM
VYQAFPYTVLVLFPALVRLDPTLTEAARTLGASPLRAFFTVVVPALRNTITATLIMVFIFALGSYLLPQLLGRPQHWTLS
VLITDQAIYQSNMPFAAAMAVFLVLVTLGLVALTVLAGRRGEAA

Sequences:

>Translated_284_residues
MRREPPRTLADYLPLFFPAGMLTIFFVVPFGTMIAVSFFQRQQGGFYTPAFVYDNYARFLSAFFGGVLGFSLMLAITVAL
CCVVLALPFTYLLTRMSRRVQVVWLVALLSVLSLSEVIIGFAWSTLFSRTAGITNILVALGLMGEAKALTPSFAAVLTGM
VYQAFPYTVLVLFPALVRLDPTLTEAARTLGASPLRAFFTVVVPALRNTITATLIMVFIFALGSYLLPQLLGRPQHWTLS
VLITDQAIYQSNMPFAAAMAVFLVLVTLGLVALTVLAGRRGEAA
>Mature_284_residues
MRREPPRTLADYLPLFFPAGMLTIFFVVPFGTMIAVSFFQRQQGGFYTPAFVYDNYARFLSAFFGGVLGFSLMLAITVAL
CCVVLALPFTYLLTRMSRRVQVVWLVALLSVLSLSEVIIGFAWSTLFSRTAGITNILVALGLMGEAKALTPSFAAVLTGM
VYQAFPYTVLVLFPALVRLDPTLTEAARTLGASPLRAFFTVVVPALRNTITATLIMVFIFALGSYLLPQLLGRPQHWTLS
VLITDQAIYQSNMPFAAAMAVFLVLVTLGLVALTVLAGRRGEAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=261, Percent_Identity=29.1187739463602, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787080, Length=198, Percent_Identity=32.3232323232323, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787713, Length=200, Percent_Identity=27.5, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30975; Mature: 30975

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRREPPRTLADYLPLFFPAGMLTIFFVVPFGTMIAVSFFQRQQGGFYTPAFVYDNYARFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAFFGGVLGFSLMLAITVALCCVVLALPFTYLLTRMSRRVQVVWLVALLSVLSLSEVIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAWSTLFSRTAGITNILVALGLMGEAKALTPSFAAVLTGMVYQAFPYTVLVLFPALVRLD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTLTEAARTLGASPLRAFFTVVVPALRNTITATLIMVFIFALGSYLLPQLLGRPQHWTLS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VLITDQAIYQSNMPFAAAMAVFLVLVTLGLVALTVLAGRRGEAA
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRREPPRTLADYLPLFFPAGMLTIFFVVPFGTMIAVSFFQRQQGGFYTPAFVYDNYARFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAFFGGVLGFSLMLAITVALCCVVLALPFTYLLTRMSRRVQVVWLVALLSVLSLSEVIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAWSTLFSRTAGITNILVALGLMGEAKALTPSFAAVLTGMVYQAFPYTVLVLFPALVRLD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTLTEAARTLGASPLRAFFTVVVPALRNTITATLIMVFIFALGSYLLPQLLGRPQHWTLS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VLITDQAIYQSNMPFAAAMAVFLVLVTLGLVALTVLAGRRGEAA
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]