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The map label for this gene is yafV [C]

Identifier: 150397605

GI number: 150397605

Start: 2480867

End: 2481643

Strand: Reverse

Name: yafV [C]

Synonym: Smed_2405

Alternate gene names: 150397605

Gene position: 2481643-2480867 (Counterclockwise)

Preceding gene: 150397606

Following gene: 150397604

Centisome position: 65.62

GC content: 62.93

Gene sequence:

>777_bases
ATGAAGCTCGCCGTTCTGCAGATGAAGAGCACCGGCGGCGACGTTGCCGCCAATCTCGCCCGCATCGGGCGGGCGGCGAT
CGACGCTTCCGGCAAGGGCGCGACCCTGCTCGTCGCGCCCGAACTCGCAATCACCGGCTATGGCGCGGGGGAAGCGATCG
TCGAACTTGCCGAGCCGCCGGATGGGCCGATCGTTCAGGAATTGCGACGCATCTCACGGCAGACCGGCACCGCCATCATC
GCCGGTTTCGCAGAGCAAAGCGGGCACACCGTCTACAACAGCGCCGTTCATGTCGACGGCGATACGGCACCGACCGTCTA
CCGGAAGTCGCATCTCTACGGCGATTACGAGCGTTCGCTGTTCAGCCCGGCCGAGCCGTCGACGCGCCTCTTCGAACATC
GGGGCGTCACCTGCGGCATGCTGATATGCTACGACGTGGAATTTCCTGAAAACGTCCGCCGGCTGGCGCTCGCGGGCGCC
GATGCGGTGCTGGTGCCGACCGCTCTTCCGGCCGGCTGGTCCGGCACCTTCATCGCCGAGCACATGATCCAGACGAGGGC
TTTCGAGAATCAGGTTTTCGTCGCTTACGCCAACCACTGCGGCTCGGACGATATGTTCTCCTTTGCGGGAATGTCGCGTA
TTGCGTCACCGGATGGCCAGGTCATGGCAAAGAGCGGCTCCACGGATGAGACCCTGATTTTCGCCGAAATCGACCCGCAG
GCATTTGCCATTTCCCGGGCGGAGAATACTTATCTCAATGACTTGCAACGCGACTGA

Upstream 100 bases:

>100_bases
GGTCCTTTTCGTCGTGACGGCTGCGGTGATCTTCAGCCTCGTCGCCCGCTTCGGAGATCTGCCGAAGCTGCTCGGCGCCA
TGTCATCGGACGGAAAGTAG

Downstream 100 bases:

>100_bases
TCTGCCGCATCGTTCTTAAATGGAGGACAATCCGTTCGCGTTGTGCCCTCTTTCGGAAACGCCTGGCCGGGTCGATATTC
TGCTCAACCGAAGTGCCCGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKLAVLQMKSTGGDVAANLARIGRAAIDASGKGATLLVAPELAITGYGAGEAIVELAEPPDGPIVQELRRISRQTGTAII
AGFAEQSGHTVYNSAVHVDGDTAPTVYRKSHLYGDYERSLFSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGA
DAVLVPTALPAGWSGTFIAEHMIQTRAFENQVFVAYANHCGSDDMFSFAGMSRIASPDGQVMAKSGSTDETLIFAEIDPQ
AFAISRAENTYLNDLQRD

Sequences:

>Translated_258_residues
MKLAVLQMKSTGGDVAANLARIGRAAIDASGKGATLLVAPELAITGYGAGEAIVELAEPPDGPIVQELRRISRQTGTAII
AGFAEQSGHTVYNSAVHVDGDTAPTVYRKSHLYGDYERSLFSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGA
DAVLVPTALPAGWSGTFIAEHMIQTRAFENQVFVAYANHCGSDDMFSFAGMSRIASPDGQVMAKSGSTDETLIFAEIDPQ
AFAISRAENTYLNDLQRD
>Mature_258_residues
MKLAVLQMKSTGGDVAANLARIGRAAIDASGKGATLLVAPELAITGYGAGEAIVELAEPPDGPIVQELRRISRQTGTAII
AGFAEQSGHTVYNSAVHVDGDTAPTVYRKSHLYGDYERSLFSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGA
DAVLVPTALPAGWSGTFIAEHMIQTRAFENQVFVAYANHCGSDDMFSFAGMSRIASPDGQVMAKSGSTDETLIFAEIDPQ
AFAISRAENTYLNDLQRD

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786412, Length=238, Percent_Identity=28.1512605042017, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=268, Percent_Identity=26.4925373134328, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17933642, Length=255, Percent_Identity=29.0196078431373, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 27574; Mature: 27574

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVLQMKSTGGDVAANLARIGRAAIDASGKGATLLVAPELAITGYGAGEAIVELAEPP
CEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCHHEEEECCCC
DGPIVQELRRISRQTGTAIIAGFAEQSGHTVYNSAVHVDGDTAPTVYRKSHLYGDYERSL
CCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECEEEECCCCCCCEEHHHHCCCCHHHHH
FSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGADAVLVPTALPAGWSGTFIAE
CCCCCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
HMIQTRAFENQVFVAYANHCGSDDMFSFAGMSRIASPDGQVMAKSGSTDETLIFAEIDPQ
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCC
AFAISRAENTYLNDLQRD
EEEEECCCCHHHHHHCCC
>Mature Secondary Structure
MKLAVLQMKSTGGDVAANLARIGRAAIDASGKGATLLVAPELAITGYGAGEAIVELAEPP
CEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCHHEEEECCCC
DGPIVQELRRISRQTGTAIIAGFAEQSGHTVYNSAVHVDGDTAPTVYRKSHLYGDYERSL
CCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECEEEECCCCCCCEEHHHHCCCCHHHHH
FSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGADAVLVPTALPAGWSGTFIAE
CCCCCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
HMIQTRAFENQVFVAYANHCGSDDMFSFAGMSRIASPDGQVMAKSGSTDETLIFAEIDPQ
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCC
AFAISRAENTYLNDLQRD
EEEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]