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The map label for this gene is emrE [H]

Identifier: 150397528

GI number: 150397528

Start: 2409962

End: 2410303

Strand: Reverse

Name: emrE [H]

Synonym: Smed_2328

Alternate gene names: 150397528

Gene position: 2410303-2409962 (Counterclockwise)

Preceding gene: 150397529

Following gene: 150397527

Centisome position: 63.73

GC content: 61.11

Gene sequence:

>342_bases
GTGAACCCCGCTACGCTCTACACCGTTCTGGTGATCGCAATCGTCTTCGAAGTCCTCGGCACCTCGGCCATGCAGGCGGC
GCAACACTTCACGCGGCTGACGCCCACCTTGCTCATGGTGTTCTGCTATGCCGTCGCCTTCTTCTTCCTGTCCTATGCGC
TCCGCTACATCCCCGTCGGGATCGCCTATGCGCTCTGGAGCGGCCTCGGAATCGTGCTGATTTCGATTGCGGGCTACGTC
GTCTTCGGCCAGAAGCTCGATCTGCCGGCGGTGCTCGGACTGGCGCTCATCATTGCCGGGGTGCTGGTGCTCAACCTCTT
CTCCAAGTCGACGTTCCATTGA

Upstream 100 bases:

>100_bases
GATCGTCCGACGCGGGCGATACTGCTTCAGAGGCTCAACGCGCTTTCGCTGGAGTGAAGGGCGAAGCTTTCCATCTGCAA
TCTCAATCGGAGACGTTCCC

Downstream 100 bases:

>100_bases
ACGCCTCGCGCCCTCCGCATTTCTGCGCGCGGCGGAAGTCTCCCTGCAATTTTTCACTACAGCCTGTCCACGGATGTTCC
CGGATATTCATTGAAATGCG

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MNPATLYTVLVIAIVFEVLGTSAMQAAQHFTRLTPTLLMVFCYAVAFFFLSYALRYIPVGIAYALWSGLGIVLISIAGYV
VFGQKLDLPAVLGLALIIAGVLVLNLFSKSTFH

Sequences:

>Translated_113_residues
MNPATLYTVLVIAIVFEVLGTSAMQAAQHFTRLTPTLLMVFCYAVAFFFLSYALRYIPVGIAYALWSGLGIVLISIAGYV
VFGQKLDLPAVLGLALIIAGVLVLNLFSKSTFH
>Mature_113_residues
MNPATLYTVLVIAIVFEVLGTSAMQAAQHFTRLTPTLLMVFCYAVAFFFLSYALRYIPVGIAYALWSGLGIVLISIAGYV
VFGQKLDLPAVLGLALIIAGVLVLNLFSKSTFH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=113, Percent_Identity=53.0973451327434, Blast_Score=119, Evalue=4e-29,
Organism=Escherichia coli, GI87082378, Length=100, Percent_Identity=36, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1787884, Length=99, Percent_Identity=32.3232323232323, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 12232; Mature: 12232

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPATLYTVLVIAIVFEVLGTSAMQAAQHFTRLTPTLLMVFCYAVAFFFLSYALRYIPVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYALWSGLGIVLISIAGYVVFGQKLDLPAVLGLALIIAGVLVLNLFSKSTFH
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPATLYTVLVIAIVFEVLGTSAMQAAQHFTRLTPTLLMVFCYAVAFFFLSYALRYIPVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYALWSGLGIVLISIAGYVVFGQKLDLPAVLGLALIIAGVLVLNLFSKSTFH
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]