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The map label for this gene is trpE(G) [H]

Identifier: 150397495

GI number: 150397495

Start: 2369676

End: 2371865

Strand: Reverse

Name: trpE(G) [H]

Synonym: Smed_2295

Alternate gene names: 150397495

Gene position: 2371865-2369676 (Counterclockwise)

Preceding gene: 150397496

Following gene: 150397494

Centisome position: 62.72

GC content: 61.96

Gene sequence:

>2190_bases
ATGGCAGCGGTAATTCTGGAAGACGGCGCGGAGTGTTATACGACCAAGGGCGGCATCGTCGTCAACCGCAGGCGGCGTGA
GGCCTCCTATACGGAGGCGATTGCCGGCTATGTCGACAGGCTGGACGAGCGGCGCGGCGCGGTCTTCTCCTCCAACTACG
AATATCCGGGGCGTTACACGCGCTGGGACACCGCCGTCGTCGACCCGCCGCTTGCCATTTCCTCCTTCGGTCGTTCGCTT
TGGATCGAAGCTTATAATGAGCGGGGCGAGGTGCTTCTCGCGCTGATCGCCGAGGATCTCAAGTCCGTTTCCGACATCAC
GCTCGGCACGCTGACCGACCGCCGACTGGATCTCACCATCAATGAGCCCGATCGTGTCTTCACCGAGGAAGAGCGTTCGA
AGATGCCGACCGTCTTCACGGTGCTTCGCGCGGTGACGGACCTCTTTCATTCGGAGGAGGATTCGAACCTCGGCCTCTAT
GGCGCCTTTGGGTATGACCTGGCCTTCCAGTTCGACGCGATCGAACTGAAGCTCACGCGCCCGGAGGATCAGCGCGACAT
GGTTCTCTTCCTGCCGGACGAGATCCTGGTCGTGGACCACTATGCGGCAAAGGCCTGGATCGATCGTTACGACTTCGCCA
AGGAGGGGCTGTCGACGGAGGACAAGGCAGCCGATATCGCGCCTGAGGCGTTCCGCAGCGTCGACAGCATTCCGCCGCAC
GGGGACCATCGCCCGGGCGAATATGCCGAGCTCGTCGTCAAGGCGAAGGAGAGCTTCCGCCGCGGCGATCTTTTCGAAGT
GGTGCCGGGACAGAAATTCTACGAGCGCTGCGATAGTCGCCCTTCCGAGATTTCCAACCGGCTGAAGGCGATCAATCCGT
CGCCCTATTCCTTCTTCATCAATCTCGGAAACCAGGAATATCTCGTCGGTGCTTCGCCGGAAATGTTCGTGCGCGTCTCC
GGCCGGCGGATAGAGACCTGCCCCATCTCCGGTACGATCAAGCGCGGCGACGATCCGATCGCAGACAGCGAGCAGATACT
GAAGCTGCTGAATTCGAAGAAGGACGAGTCCGAACTTACCATGTGCTCGGACGTGGACCGCAACGACAAGAGCCGCGTCT
GCGTGCCTGGCTCGGTGAAAGTGATCGGCCGGCGGCAGATCGAGATGTATTCGCGGCTGATCCATACGGTCGATCACATC
GAGGGCCGCCTGCGGGACGATATGGACGCCTTCGACGGATTTCTGAGCCATGCCTGGGCGGTGACGGTGACCGGCGCACC
GAAGCTCTGGGCGATGCGGTTCATCGAGAGCCACGAGAAGAGCCCACGCGCCTGGTACGGGGGTGCGATCGGCATGGTAG
GCTTCAACGGCGACATGAATACCGGTCTGACATTGCGTACGATCCGCATCAAGGACGGGATTGCCGAGGTGAGGGCGGGT
GCGACGCTGCTCTATGATTCCAATCCGGAAGAAGAAGAAGCCGAAACCGAACTGAAGGCCTCTGCCATGATCGCAGCCAT
CCGTGACGCGAAATCCGCAAACAGCGCCAACGCAGCGCGCGATGTTGCCGCCGTCGGCGCCGGGGTCAGCATCCTCCTCG
TGGACCACGAGGACAGCTTCGTCCACACCCTGGCGAACTATTTCCGGCAGACGGGAGCGACCGTCACCACGGTGCGCACG
CCGGTGGCCGAGGAAATCTTCGATAGGGTCAAGCCTGACCTCGTCGTGCTTTCGCCCGGTCCCGGCAACCCGAAAGACTT
CGATTGCAAGGCGACGATCAAGAAGGCGCGGGCGCGCGAACTGCCGATCTTCGGCGTGTGCCTCGGGCTGCAGGCGCTTG
CGGAGGCCTATGGCGGCAACTTGCGGCAACTGGCGGTCCCGATGCATGGCAAGCCCTCGCGCATTCGCGTCCTCGAACCC
GGCATCGTCTTTTCGGGCCTCGGCAAGGAGGTGACCGTCGGGCGCTATCATTCGATCTTCGCCGATCCGTCCGGCCTTCC
GCGCGAGTTTATGATTACGGCCGAAAGCGAGGACGGCACGATCATGGGCATCGAGCATACGAAGGAACCGGTGGCGGCGG
TGCAATTCCATCCGGAATCGATCATGACGCTCGGCGGCGACGCCGGCATGCGGATGATCGAGAACGTGGTGGCCCATCTT
GCAAAACGGGCGAAGATCAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
AGTCATGCGTGATTGAGAGTTGGAGCCGCCCGGAGATTTCGAGGCGGCTTTTTCGTATTCTGGCCGCGTGGGAAGACCGG
GCTTTTACGGAGCGAGACGA

Downstream 100 bases:

>100_bases
CCTTTTCGCGGCCGGTCTTTGGCACTCGGCCGTTTGACAAGCGATCCGTTATCGCCCATATGGCTCCCATCATCCGCCTG
CGCGAAAATCCGCGCGGGCG

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAAVILEDGAECYTTKGGIVVNRRRREASYTEAIAGYVDRLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLAISSFGRSL
WIEAYNERGEVLLALIAEDLKSVSDITLGTLTDRRLDLTINEPDRVFTEEERSKMPTVFTVLRAVTDLFHSEEDSNLGLY
GAFGYDLAFQFDAIELKLTRPEDQRDMVLFLPDEILVVDHYAAKAWIDRYDFAKEGLSTEDKAADIAPEAFRSVDSIPPH
GDHRPGEYAELVVKAKESFRRGDLFEVVPGQKFYERCDSRPSEISNRLKAINPSPYSFFINLGNQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCVPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
ATLLYDSNPEEEEAETELKASAMIAAIRDAKSANSANAARDVAAVGAGVSILLVDHEDSFVHTLANYFRQTGATVTTVRT
PVAEEIFDRVKPDLVVLSPGPGNPKDFDCKATIKKARARELPIFGVCLGLQALAEAYGGNLRQLAVPMHGKPSRIRVLEP
GIVFSGLGKEVTVGRYHSIFADPSGLPREFMITAESEDGTIMGIEHTKEPVAAVQFHPESIMTLGGDAGMRMIENVVAHL
AKRAKIKAA

Sequences:

>Translated_729_residues
MAAVILEDGAECYTTKGGIVVNRRRREASYTEAIAGYVDRLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLAISSFGRSL
WIEAYNERGEVLLALIAEDLKSVSDITLGTLTDRRLDLTINEPDRVFTEEERSKMPTVFTVLRAVTDLFHSEEDSNLGLY
GAFGYDLAFQFDAIELKLTRPEDQRDMVLFLPDEILVVDHYAAKAWIDRYDFAKEGLSTEDKAADIAPEAFRSVDSIPPH
GDHRPGEYAELVVKAKESFRRGDLFEVVPGQKFYERCDSRPSEISNRLKAINPSPYSFFINLGNQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCVPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
ATLLYDSNPEEEEAETELKASAMIAAIRDAKSANSANAARDVAAVGAGVSILLVDHEDSFVHTLANYFRQTGATVTTVRT
PVAEEIFDRVKPDLVVLSPGPGNPKDFDCKATIKKARARELPIFGVCLGLQALAEAYGGNLRQLAVPMHGKPSRIRVLEP
GIVFSGLGKEVTVGRYHSIFADPSGLPREFMITAESEDGTIMGIEHTKEPVAAVQFHPESIMTLGGDAGMRMIENVVAHL
AKRAKIKAA
>Mature_728_residues
AAVILEDGAECYTTKGGIVVNRRRREASYTEAIAGYVDRLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLAISSFGRSLW
IEAYNERGEVLLALIAEDLKSVSDITLGTLTDRRLDLTINEPDRVFTEEERSKMPTVFTVLRAVTDLFHSEEDSNLGLYG
AFGYDLAFQFDAIELKLTRPEDQRDMVLFLPDEILVVDHYAAKAWIDRYDFAKEGLSTEDKAADIAPEAFRSVDSIPPHG
DHRPGEYAELVVKAKESFRRGDLFEVVPGQKFYERCDSRPSEISNRLKAINPSPYSFFINLGNQEYLVGASPEMFVRVSG
RRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCVPGSVKVIGRRQIEMYSRLIHTVDHIE
GRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAGA
TLLYDSNPEEEEAETELKASAMIAAIRDAKSANSANAARDVAAVGAGVSILLVDHEDSFVHTLANYFRQTGATVTTVRTP
VAEEIFDRVKPDLVVLSPGPGNPKDFDCKATIKKARARELPIFGVCLGLQALAEAYGGNLRQLAVPMHGKPSRIRVLEPG
IVFSGLGKEVTVGRYHSIFADPSGLPREFMITAESEDGTIMGIEHTKEPVAAVQFHPESIMTLGGDAGMRMIENVVAHLA
KRAKIKAA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=410, Percent_Identity=31.7073170731707, Blast_Score=185, Evalue=9e-48,
Organism=Escherichia coli, GI1788114, Length=429, Percent_Identity=29.6037296037296, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789760, Length=188, Percent_Identity=42.0212765957447, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787517, Length=192, Percent_Identity=36.9791666666667, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1786809, Length=223, Percent_Identity=29.5964125560538, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786215, Length=151, Percent_Identity=31.7880794701987, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=271, Percent_Identity=36.90036900369, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322638, Length=188, Percent_Identity=36.7021276595745, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324361, Length=117, Percent_Identity=32.4786324786325, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 80626; Mature: 80495

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVILEDGAECYTTKGGIVVNRRRREASYTEAIAGYVDRLDERRGAVFSSNYEYPGRYT
CEEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
RWDTAVVDPPLAISSFGRSLWIEAYNERGEVLLALIAEDLKSVSDITLGTLTDRRLDLTI
CCCCEECCCCHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCEEEEECCCEEEEEE
NEPDRVFTEEERSKMPTVFTVLRAVTDLFHSEEDSNLGLYGAFGYDLAFQFDAIELKLTR
CCCCHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEEEEC
PEDQRDMVLFLPDEILVVDHYAAKAWIDRYDFAKEGLSTEDKAADIAPEAFRSVDSIPPH
CCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCC
GDHRPGEYAELVVKAKESFRRGDLFEVVPGQKFYERCDSRPSEISNRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGNQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
EECCCEEEEECCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHH
MCSDVDRNDKSRVCVPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
HHHCCCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIESHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLYDSNPEEEEAETELKASAMIAAIRDAKSANSANAARDVAAVGAGVSILLVDHEDSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGATVTTVRTPVAEEIFDRVKPDLVVLSPGPGNPKDFDCKATIKKARARE
HHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
LPIFGVCLGLQALAEAYGGNLRQLAVPMHGKPSRIRVLEPGIVFSGLGKEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEECCCCEEEECCHHEEE
ADPSGLPREFMITAESEDGTIMGIEHTKEPVAAVQFHPESIMTLGGDAGMRMIENVVAHL
CCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCHHHEEECCHHHHHHHHHHHHHH
AKRAKIKAA
HHHHCCCCC
>Mature Secondary Structure 
AAVILEDGAECYTTKGGIVVNRRRREASYTEAIAGYVDRLDERRGAVFSSNYEYPGRYT
EEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
RWDTAVVDPPLAISSFGRSLWIEAYNERGEVLLALIAEDLKSVSDITLGTLTDRRLDLTI
CCCCEECCCCHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCEEEEECCCEEEEEE
NEPDRVFTEEERSKMPTVFTVLRAVTDLFHSEEDSNLGLYGAFGYDLAFQFDAIELKLTR
CCCCHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEEEEC
PEDQRDMVLFLPDEILVVDHYAAKAWIDRYDFAKEGLSTEDKAADIAPEAFRSVDSIPPH
CCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCC
GDHRPGEYAELVVKAKESFRRGDLFEVVPGQKFYERCDSRPSEISNRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGNQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
EECCCEEEEECCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHH
MCSDVDRNDKSRVCVPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
HHHCCCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIESHEKSPRAWYGGAIGMVGFNGDMNTGLTLRTIRIKDGIAEVRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLYDSNPEEEEAETELKASAMIAAIRDAKSANSANAARDVAAVGAGVSILLVDHEDSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGATVTTVRTPVAEEIFDRVKPDLVVLSPGPGNPKDFDCKATIKKARARE
HHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
LPIFGVCLGLQALAEAYGGNLRQLAVPMHGKPSRIRVLEPGIVFSGLGKEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEECCCCEEEECCHHEEE
ADPSGLPREFMITAESEDGTIMGIEHTKEPVAAVQFHPESIMTLGGDAGMRMIENVVAHL
CCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCHHHEEECCHHHHHHHHHHHHHH
AKRAKIKAA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]