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The map label for this gene is prpB [C]

Identifier: 150397458

GI number: 150397458

Start: 2335436

End: 2336266

Strand: Reverse

Name: prpB [C]

Synonym: Smed_2258

Alternate gene names: 150397458

Gene position: 2336266-2335436 (Counterclockwise)

Preceding gene: 150397459

Following gene: 150397451

Centisome position: 61.77

GC content: 64.5

Gene sequence:

>831_bases
ATGGAAAATCAACAAGACAAGCACAGGGCATTCCGCGCCCTGCATGATCGTCAGGGCGCTTTCGTAATTCCCAATCCCTG
GGATGCAGGTTCTGCGCGAATCCTCGCGGCGCTCGGCTTCGAGGCGCTGGCGACGACGAGCGCCGGCCTTGCCTTTTCCA
TTGGCCGAAGGGATTCGTCGGCGGCTCTCTCGCGGGACGTCATATTGCAGAATGCCCGCGACATCGTCGGTGCGACCGAT
TTGCCCGTGTCCGCCGATCTGGAGGACGGATTTGGTAAAGATCCCCTGTCCTGCGCCGAAACGATTTCGCTGGCGGCAGG
CGTCGGGCTCGTCGGCGGCTCGATCGAGGATGCGACCGGCGATGCCGCCGACCGCGTTTTCGAGTTCAGCCTGGCGGTGG
AACGCGTTGCCGCGGCCGCGGAGGAGGCGCGCAAACACCCCTTCGTCCTGACGGCCCGGGCGGAGAACTTCCTGTGCGGG
CGGTACGACCTCGACGACACCATCCGGCGCCTCGTCGCATTCGAGGAAGCCGGCGCGGATGTGCTCTACGCTCCAGGGCT
GCCGGATATCGAAGCCATCCGCACGGTCTGCTCTGCCGTCAAGCGCCCGGTCAACGTCGTCATGGGGCTGGCGGGGCAGG
TGTACTCGGTGGAACAGCTGCAAGAGGCAGGTGTGAAGCGCATCAGTGTCGGCGGCTCGTTTGCACGGGCGGCCCTTGCA
GGACTCATGAGCGCGGCACGGGAGGTCAAGGAGCGCGGCACGTTCAACTATTCCAAAAGTGCCATGCCAGCCGCCGAAGC
GGCATCCTATATGCTCGACGCCAAGAGATGA

Upstream 100 bases:

>100_bases
CGCGTGCGCGGTGCAGGCTCGAGGCTGAAGCTCTCGAACTGTTCGCTTTTTGTTCGTTTCATGCTATCGCGTCTCGTCTG
CAGCGGCGAGAGTAGAGCAC

Downstream 100 bases:

>100_bases
ATGGCGGTCGCGTTCACAGGGATCAGCAGCGCCGCTTCTGCGGCGCAAGCGTCAGTCGAACAGGCTCGAAACCGATTCTT
CCTGGCTCGTGCGGCTAATC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MENQQDKHRAFRALHDRQGAFVIPNPWDAGSARILAALGFEALATTSAGLAFSIGRRDSSAALSRDVILQNARDIVGATD
LPVSADLEDGFGKDPLSCAETISLAAGVGLVGGSIEDATGDAADRVFEFSLAVERVAAAAEEARKHPFVLTARAENFLCG
RYDLDDTIRRLVAFEEAGADVLYAPGLPDIEAIRTVCSAVKRPVNVVMGLAGQVYSVEQLQEAGVKRISVGGSFARAALA
GLMSAAREVKERGTFNYSKSAMPAAEAASYMLDAKR

Sequences:

>Translated_276_residues
MENQQDKHRAFRALHDRQGAFVIPNPWDAGSARILAALGFEALATTSAGLAFSIGRRDSSAALSRDVILQNARDIVGATD
LPVSADLEDGFGKDPLSCAETISLAAGVGLVGGSIEDATGDAADRVFEFSLAVERVAAAAEEARKHPFVLTARAENFLCG
RYDLDDTIRRLVAFEEAGADVLYAPGLPDIEAIRTVCSAVKRPVNVVMGLAGQVYSVEQLQEAGVKRISVGGSFARAALA
GLMSAAREVKERGTFNYSKSAMPAAEAASYMLDAKR
>Mature_276_residues
MENQQDKHRAFRALHDRQGAFVIPNPWDAGSARILAALGFEALATTSAGLAFSIGRRDSSAALSRDVILQNARDIVGATD
LPVSADLEDGFGKDPLSCAETISLAAGVGLVGGSIEDATGDAADRVFEFSLAVERVAAAAEEARKHPFVLTARAENFLCG
RYDLDDTIRRLVAFEEAGADVLYAPGLPDIEAIRTVCSAVKRPVNVVMGLAGQVYSVEQLQEAGVKRISVGGSFARAALA
GLMSAAREVKERGTFNYSKSAMPAAEAASYMLDAKR

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=228, Percent_Identity=26.7543859649123, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29163; Mature: 29163

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQQDKHRAFRALHDRQGAFVIPNPWDAGSARILAALGFEALATTSAGLAFSIGRRDSS
CCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCH
AALSRDVILQNARDIVGATDLPVSADLEDGFGKDPLSCAETISLAAGVGLVGGSIEDATG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
DAADRVFEFSLAVERVAAAAEEARKHPFVLTARAENFLCGRYDLDDTIRRLVAFEEAGAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCC
VLYAPGLPDIEAIRTVCSAVKRPVNVVMGLAGQVYSVEQLQEAGVKRISVGGSFARAALA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHHHHHHHH
GLMSAAREVKERGTFNYSKSAMPAAEAASYMLDAKR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MENQQDKHRAFRALHDRQGAFVIPNPWDAGSARILAALGFEALATTSAGLAFSIGRRDSS
CCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCH
AALSRDVILQNARDIVGATDLPVSADLEDGFGKDPLSCAETISLAAGVGLVGGSIEDATG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
DAADRVFEFSLAVERVAAAAEEARKHPFVLTARAENFLCGRYDLDDTIRRLVAFEEAGAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCC
VLYAPGLPDIEAIRTVCSAVKRPVNVVMGLAGQVYSVEQLQEAGVKRISVGGSFARAALA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHHHHHHHH
GLMSAAREVKERGTFNYSKSAMPAAEAASYMLDAKR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]