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The map label for this gene is potD [H]

Identifier: 150397369

GI number: 150397369

Start: 2238792

End: 2239952

Strand: Direct

Name: potD [H]

Synonym: Smed_2168

Alternate gene names: 150397369

Gene position: 2238792-2239952 (Clockwise)

Preceding gene: 150397368

Following gene: 150397370

Centisome position: 59.2

GC content: 60.98

Gene sequence:

>1161_bases
ATGACCAAATGGTACAGAGAGAATGCCCCGATCACTGCGGACAAGCTTGCCGACGAGCTGATGCGCCTGAAGCGCGGCTC
CGTTTCGCGCCGTCATTTCCTTGGTGTCACCGGCCTCGGCCTCGCGACCGCCGTCCTGGCGCGTCAGCCGGGCCTGTTCA
ACTCCACCGCTTACGCCGAAGACCTTGGCACGCAGATGTCGATCGCGACCTGGCCCAACTATCACGATCCCGCCACCTTC
GAAGCCTTCACCGCTGCGACGGGCGTCGCCGTCGAAGTCAATGTCTTCGGCTCCAACGAGGAAATGCTTGCAAAGCTCCA
GGCCGGCGGAACCGGCTGGGACCTCTTCGTGCCCACGAACTACACGATCTCCACCTATGTGAAGCTTGGCCTGATCGACG
AGCTCGATCTCTCCAAGCTGCCGAACTACGATGCCTCGACGGAGAACGCGCGATTCACCAGCGAAGGCGTCGTCGAGGGC
AAGACCTATGCCGTGCCGAAGAATTGGGGAACGACCGGAATTGCGGTGAATTCCGACAAGATCAAGGCTCCGGTTGCGAG
CTGGAAGGACTTCTTCGAGATCGCGATGACGGAAGGCGACGGCCGCGCCATGGTGCACGACTATCAGCTCACCACCATCG
GCAATGCGCTCGTTTCCCTCGGTTTCTCCTTCAATTCGATCAAGCCGGAGGAGCTCGCCAAGGCCGAAGAGCTCCTGATC
AAGGTCAAGCCGCATCTCTACGCGATCAACAGCGACTATCAGCCGGCGATGCGCGCCACCGATGCATGGATGACCATGTG
CTGGACCAATGACGGAGCGCAATTGAACCGCGACATGCCGGAGATCAAGTTCGTCCTCGGAAAGGATGGCGGCGAAATCT
GGTCGGATTTCTACGCGATCCCGAAGAGTGCGGCGAACAAGGCCGCCGGTTACGCGCTGCTCGATTATCTGATGGCCCCG
AATAGCGCGGTCAAGGAACACATCGCCAACGGCGCACCGACGACCGACAGCCGGGTTCTCAAGCTGCTGCCTGCAGACGT
CACCTCGAACAAGATCGTCTATCCGGACGAAGCGGCTCTCAGCCCACTCGAATTCGGCGCTGCGGTGACGCTCACCGACC
CGGGCCGGGCCGAGCTGATGGCGCGCTTCAAATCGGCTTGA

Upstream 100 bases:

>100_bases
AGGATCGGGCTCAGCTTCGACCCGAATGCACTCCATGTCTTTCCGGCCTGAAGGGCCCGATCTGCCGCAATCAATGACAT
CAAGATAAGGGAACAGCATC

Downstream 100 bases:

>100_bases
GTTGGGAGTGACCGCCCCTCATCCGCCTGCCGGCACCTTCTCCCCGTAGTGACGGGGAGAAGGTGCCGGCAGGTGATGAG
GGGCCACTTCAACGATCAGA

Product: putative periplasmic binding ABC transporter protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTKWYRENAPITADKLADELMRLKRGSVSRRHFLGVTGLGLATAVLARQPGLFNSTAYAEDLGTQMSIATWPNYHDPATF
EAFTAATGVAVEVNVFGSNEEMLAKLQAGGTGWDLFVPTNYTISTYVKLGLIDELDLSKLPNYDASTENARFTSEGVVEG
KTYAVPKNWGTTGIAVNSDKIKAPVASWKDFFEIAMTEGDGRAMVHDYQLTTIGNALVSLGFSFNSIKPEELAKAEELLI
KVKPHLYAINSDYQPAMRATDAWMTMCWTNDGAQLNRDMPEIKFVLGKDGGEIWSDFYAIPKSAANKAAGYALLDYLMAP
NSAVKEHIANGAPTTDSRVLKLLPADVTSNKIVYPDEAALSPLEFGAAVTLTDPGRAELMARFKSA

Sequences:

>Translated_386_residues
MTKWYRENAPITADKLADELMRLKRGSVSRRHFLGVTGLGLATAVLARQPGLFNSTAYAEDLGTQMSIATWPNYHDPATF
EAFTAATGVAVEVNVFGSNEEMLAKLQAGGTGWDLFVPTNYTISTYVKLGLIDELDLSKLPNYDASTENARFTSEGVVEG
KTYAVPKNWGTTGIAVNSDKIKAPVASWKDFFEIAMTEGDGRAMVHDYQLTTIGNALVSLGFSFNSIKPEELAKAEELLI
KVKPHLYAINSDYQPAMRATDAWMTMCWTNDGAQLNRDMPEIKFVLGKDGGEIWSDFYAIPKSAANKAAGYALLDYLMAP
NSAVKEHIANGAPTTDSRVLKLLPADVTSNKIVYPDEAALSPLEFGAAVTLTDPGRAELMARFKSA
>Mature_385_residues
TKWYRENAPITADKLADELMRLKRGSVSRRHFLGVTGLGLATAVLARQPGLFNSTAYAEDLGTQMSIATWPNYHDPATFE
AFTAATGVAVEVNVFGSNEEMLAKLQAGGTGWDLFVPTNYTISTYVKLGLIDELDLSKLPNYDASTENARFTSEGVVEGK
TYAVPKNWGTTGIAVNSDKIKAPVASWKDFFEIAMTEGDGRAMVHDYQLTTIGNALVSLGFSFNSIKPEELAKAEELLIK
VKPHLYAINSDYQPAMRATDAWMTMCWTNDGAQLNRDMPEIKFVLGKDGGEIWSDFYAIPKSAANKAAGYALLDYLMAPN
SAVKEHIANGAPTTDSRVLKLLPADVTSNKIVYPDEAALSPLEFGAAVTLTDPGRAELMARFKSA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=335, Percent_Identity=31.6417910447761, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787078, Length=313, Percent_Identity=30.9904153354633, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42005; Mature: 41874

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWYRENAPITADKLADELMRLKRGSVSRRHFLGVTGLGLATAVLARQPGLFNSTAYAE
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECHHHHHHHHHHCCCCCCCCCHHHH
DLGTQMSIATWPNYHDPATFEAFTAATGVAVEVNVFGSNEEMLAKLQAGGTGWDLFVPTN
HHCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEECCC
YTISTYVKLGLIDELDLSKLPNYDASTENARFTSEGVVEGKTYAVPKNWGTTGIAVNSDK
CEEEEEEEECCCCCCCHHHCCCCCCCCCCCEECCCCCCCCCEEECCCCCCCCEEEECCCC
IKAPVASWKDFFEIAMTEGDGRAMVHDYQLTTIGNALVSLGFSFNSIKPEELAKAEELLI
CCCCHHHHHHHEEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEE
KVKPHLYAINSDYQPAMRATDAWMTMCWTNDGAQLNRDMPEIKFVLGKDGGEIWSDFYAI
EECCEEEEECCCCCHHHHHCCCEEEEEECCCCCHHCCCCCCEEEEEECCCHHHHHHHHHC
PKSAANKAAGYALLDYLMAPNSAVKEHIANGAPTTDSRVLKLLPADVTSNKIVYPDEAAL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCHHHC
SPLEFGAAVTLTDPGRAELMARFKSA
CHHHCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
TKWYRENAPITADKLADELMRLKRGSVSRRHFLGVTGLGLATAVLARQPGLFNSTAYAE
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECHHHHHHHHHHCCCCCCCCCHHHH
DLGTQMSIATWPNYHDPATFEAFTAATGVAVEVNVFGSNEEMLAKLQAGGTGWDLFVPTN
HHCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEECCC
YTISTYVKLGLIDELDLSKLPNYDASTENARFTSEGVVEGKTYAVPKNWGTTGIAVNSDK
CEEEEEEEECCCCCCCHHHCCCCCCCCCCCEECCCCCCCCCEEECCCCCCCCEEEECCCC
IKAPVASWKDFFEIAMTEGDGRAMVHDYQLTTIGNALVSLGFSFNSIKPEELAKAEELLI
CCCCHHHHHHHEEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEE
KVKPHLYAINSDYQPAMRATDAWMTMCWTNDGAQLNRDMPEIKFVLGKDGGEIWSDFYAI
EECCEEEEECCCCCHHHHHCCCEEEEEECCCCCHHCCCCCCEEEEEECCCHHHHHHHHHC
PKSAANKAAGYALLDYLMAPNSAVKEHIANGAPTTDSRVLKLLPADVTSNKIVYPDEAAL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCHHHC
SPLEFGAAVTLTDPGRAELMARFKSA
CHHHCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]