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The map label for this gene is rna [H]

Identifier: 150397319

GI number: 150397319

Start: 2181993

End: 2182763

Strand: Reverse

Name: rna [H]

Synonym: Smed_2118

Alternate gene names: 150397319

Gene position: 2182763-2181993 (Counterclockwise)

Preceding gene: 150397324

Following gene: 150397318

Centisome position: 57.72

GC content: 62.26

Gene sequence:

>771_bases
ATGCAGGCAAGGGAATGGGTAAATCTGGTGAGGGTAGCCGCGGTGGCATTGGGAATGGCGAATGCTTCCCTCGCGGCGGC
TCAGGAGAGTTCGGCGGGCGAGAATGAAGCTGGGGGGGCGCCGAGGACCGAATATATCCTTGCCGTAAGCTGGCAACCGG
GTTTCTGCGAGACGCGGCCGGATCGCAAGGAGTGCGTCGATCAGACAGCGAGGCGGTTCGATGCGACGCATTTCTCTCTT
CATGGCCTGTGGCCGCTCAAGAAGAGCTATTGCGGTGTGGAAGCGGAGCTCAAAGTGCGCGACCGTAAGGGCGATTGGCT
GGATCTGCCGGAAGTCGCCGTGCAGGACGAGACGGCCAAGCGCCTGCTCGTTGCCATGCCGGGCGTCAAGTCGGGCCTCG
ACCGCCACCAATGGTTGCGCAACGGCACGTGCCACACCGCGAATGCCGAGGACTATTTCGCACTGCAGCTGCGGCTTCTG
GACGAGATCAACAGCTCGGCGGTACGTACGCTCTTTGCCGATCAGATCGGCAAAGAACTCGATGAAAAGCAGATCAAGCA
GGCCTTCGATCAAAGCTTCGGGGCTGGCGCCGGCGAGCGGGTGCGTATGCGCTGCCAATCGGTGAACGGCCGCAGCGTGA
TCACCGGACTGACGATCGGGCTTTCGGGCGAACTCTCGGGTAAAGCGGAGCTTGCGGAGCTGATTCAGGCGGCAGGTCCG
ACGGAGTTCAAATGCGGGAAGGGTATTGCAGCCGCTGCAGGCCGGGGCTGA

Upstream 100 bases:

>100_bases
CGAATTCGCAGGGAGTCCGGCAGTACCGATTCAAGGTTGCAATTTACTCGCATATGTAGTTATTTGTTCCTGTTTTGTTT
TCTTTCGGAGGATCGGCGCA

Downstream 100 bases:

>100_bases
TCGGTCGCAAGTCGCATTCGGTGGGTGGCGGGAGCCGTCGAGCAGAGGGCCTTTGGCGTTGCCCCGCCACCATATTTCGC
CTACATAGCGGGCAAAATTT

Product: putative ribonuclease

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQAREWVNLVRVAAVALGMANASLAAAQESSAGENEAGGAPRTEYILAVSWQPGFCETRPDRKECVDQTARRFDATHFSL
HGLWPLKKSYCGVEAELKVRDRKGDWLDLPEVAVQDETAKRLLVAMPGVKSGLDRHQWLRNGTCHTANAEDYFALQLRLL
DEINSSAVRTLFADQIGKELDEKQIKQAFDQSFGAGAGERVRMRCQSVNGRSVITGLTIGLSGELSGKAELAELIQAAGP
TEFKCGKGIAAAAGRG

Sequences:

>Translated_256_residues
MQAREWVNLVRVAAVALGMANASLAAAQESSAGENEAGGAPRTEYILAVSWQPGFCETRPDRKECVDQTARRFDATHFSL
HGLWPLKKSYCGVEAELKVRDRKGDWLDLPEVAVQDETAKRLLVAMPGVKSGLDRHQWLRNGTCHTANAEDYFALQLRLL
DEINSSAVRTLFADQIGKELDEKQIKQAFDQSFGAGAGERVRMRCQSVNGRSVITGLTIGLSGELSGKAELAELIQAAGP
TEFKCGKGIAAAAGRG
>Mature_256_residues
MQAREWVNLVRVAAVALGMANASLAAAQESSAGENEAGGAPRTEYILAVSWQPGFCETRPDRKECVDQTARRFDATHFSL
HGLWPLKKSYCGVEAELKVRDRKGDWLDLPEVAVQDETAKRLLVAMPGVKSGLDRHQWLRNGTCHTANAEDYFALQLRLL
DEINSSAVRTLFADQIGKELDEKQIKQAFDQSFGAGAGERVRMRCQSVNGRSVITGLTIGLSGELSGKAELAELIQAAGP
TEFKCGKGIAAAAGRG

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=265, Percent_Identity=28.6792452830189, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 27731; Mature: 27731

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00530 RNASE_T2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAREWVNLVRVAAVALGMANASLAAAQESSAGENEAGGAPRTEYILAVSWQPGFCETRP
CCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
DRKECVDQTARRFDATHFSLHGLWPLKKSYCGVEAELKVRDRKGDWLDLPEVAVQDETAK
CHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCHHHH
RLLVAMPGVKSGLDRHQWLRNGTCHTANAEDYFALQLRLLDEINSSAVRTLFADQIGKEL
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEKQIKQAFDQSFGAGAGERVRMRCQSVNGRSVITGLTIGLSGELSGKAELAELIQAAGP
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCC
TEFKCGKGIAAAAGRG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAREWVNLVRVAAVALGMANASLAAAQESSAGENEAGGAPRTEYILAVSWQPGFCETRP
CCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
DRKECVDQTARRFDATHFSLHGLWPLKKSYCGVEAELKVRDRKGDWLDLPEVAVQDETAK
CHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCHHHH
RLLVAMPGVKSGLDRHQWLRNGTCHTANAEDYFALQLRLLDEINSSAVRTLFADQIGKEL
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEKQIKQAFDQSFGAGAGERVRMRCQSVNGRSVITGLTIGLSGELSGKAELAELIQAAGP
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCC
TEFKCGKGIAAAAGRG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]