Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 150397308

GI number: 150397308

Start: 2168451

End: 2169083

Strand: Direct

Name: rhtB [H]

Synonym: Smed_2107

Alternate gene names: 150397308

Gene position: 2168451-2169083 (Clockwise)

Preceding gene: 150397307

Following gene: 150397309

Centisome position: 57.34

GC content: 61.77

Gene sequence:

>633_bases
ATGTCCTTCGAACACTGGTTCGCCTTCGCCGCCGCATCGGCCGTGCTGCTTGCAATTCCCGGCCCCACTATTCTTCTCGT
AATCTCCTACGCGCTCGGCCATGGCCGAAAGATTCCGGGAGCGACGGTCGCAGGTGTCGCCCTTGGTGATTTCACGGCAA
TGACCGCATCGATGCTCGGTCTTGGCGCCCTGCTCGCAACCTCGGCCGCCGTTTTCACCGTATTGAAGTGGATAGGCGCT
GCCTATCTTGTCTGGCTCGGAATCAAGCTCTGGAAGGCGCCCGTCGAAAGCGACACGGGCAGCCTCCGGAAGAGTCCCGT
CGAAAGGCCATCGCGAATCTTCATCCATACCTATGCGGTCACCGCGCTCAATCCGAAAAGCATTCTCTTCTTCGTCGCTT
TCCTGCCCCAGTTCCTCGATCTGTCTCGGCCGCTTTTCACCCAGATGGCCATCTTCGAGACGACCTTCCTGGTACTCGCG
ACAGTCAACGCGGCGCTCTACGCCTGGCTTGCCGCCGCGGCCGGCAGCACCATCCGCAGGCCCAGTGTTCGGCGCATCGT
TAACCGCGTCGGCGGTTCGCTGCTTATCGGCGCCGGCCTGCTGACGGCCGGCCTGAAACGCGTCACGTCTTGA

Upstream 100 bases:

>100_bases
GAACCGCTTCCTGCCCGAATACGTTTAAGACAGCCGTTGACGGCGGCGCGCGGGCATGGCTCCCTCCTTTTCAGAAGCCA
TTCAAACGGGGAGCCTCTTG

Downstream 100 bases:

>100_bases
AAGCTGCGCTCGCCTGCTTGCCGCCACCGGCGGCATAGGTTAATGGAAATTCACGCGAAGCGGGTGGCACCCGCTGATTT
GCCGGGGCTGCGGGGCCCAG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIPGATVAGVALGDFTAMTASMLGLGALLATSAAVFTVLKWIGA
AYLVWLGIKLWKAPVESDTGSLRKSPVERPSRIFIHTYAVTALNPKSILFFVAFLPQFLDLSRPLFTQMAIFETTFLVLA
TVNAALYAWLAAAAGSTIRRPSVRRIVNRVGGSLLIGAGLLTAGLKRVTS

Sequences:

>Translated_210_residues
MSFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIPGATVAGVALGDFTAMTASMLGLGALLATSAAVFTVLKWIGA
AYLVWLGIKLWKAPVESDTGSLRKSPVERPSRIFIHTYAVTALNPKSILFFVAFLPQFLDLSRPLFTQMAIFETTFLVLA
TVNAALYAWLAAAAGSTIRRPSVRRIVNRVGGSLLIGAGLLTAGLKRVTS
>Mature_209_residues
SFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIPGATVAGVALGDFTAMTASMLGLGALLATSAAVFTVLKWIGAA
YLVWLGIKLWKAPVESDTGSLRKSPVERPSRIFIHTYAVTALNPKSILFFVAFLPQFLDLSRPLFTQMAIFETTFLVLAT
VNAALYAWLAAAAGSTIRRPSVRRIVNRVGGSLLIGAGLLTAGLKRVTS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=207, Percent_Identity=33.8164251207729, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1788099, Length=141, Percent_Identity=31.2056737588652, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22289; Mature: 22157

Theoretical pI: Translated: 11.36; Mature: 11.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIPGATVAGVALGDFTAMTASMLG
CCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGALLATSAAVFTVLKWIGAAYLVWLGIKLWKAPVESDTGSLRKSPVERPSRIFIHTYAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEEEE
TALNPKSILFFVAFLPQFLDLSRPLFTQMAIFETTFLVLATVNAALYAWLAAAAGSTIRR
EEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSVRRIVNRVGGSLLIGAGLLTAGLKRVTS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIPGATVAGVALGDFTAMTASMLG
CHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGALLATSAAVFTVLKWIGAAYLVWLGIKLWKAPVESDTGSLRKSPVERPSRIFIHTYAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEEEE
TALNPKSILFFVAFLPQFLDLSRPLFTQMAIFETTFLVLATVNAALYAWLAAAAGSTIRR
EEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSVRRIVNRVGGSLLIGAGLLTAGLKRVTS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]