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The map label for this gene is pfp [H]

Identifier: 150397307

GI number: 150397307

Start: 2167101

End: 2168315

Strand: Direct

Name: pfp [H]

Synonym: Smed_2106

Alternate gene names: 150397307

Gene position: 2167101-2168315 (Clockwise)

Preceding gene: 150397302

Following gene: 150397308

Centisome position: 57.3

GC content: 62.3

Gene sequence:

>1215_bases
ATGGCCAAGAAGAAAGTCGCAATGCTGACGGCGGGCGGGCTTGCACCCTGCCTTTCATCCGCTGTCGGCGGGCTGATCGG
ACGCTACACGGACGTCGCGCCGGACTATGAGCTCGTGGCCTATCGTTCCGGCTATCAGGGCCTGCTTCTCGCCGACCGGA
TCGAAATTACCCCGGCAATGCGCGAGCGCGCGCATGTGCTTCACCGGCACGGCGGATCGCCGATCGGCAATAGCCGGGTG
AAGCTTACGAATACCGCAGATTGCGTGAAGCGCGGCTTGGTCAAGGAAGGTGAAAACCCGCTTCGCGTGGCCGCAGAAAG
GCTCGCCTCCGACGGCATCAGCATTCTGCACACGATCGGCGGCGACGACACCAACACCACCGCAGCCGACCTCGCGGCCT
ATCTGGGCGCCAACGGCTACGATCTGACGGTCGTCGGCCTGCCGAAGACGGTCGACAACGACGTCGTGCCGATCCGTCAG
TCGCTCGGCGCCTGGACAGCGGCCGAATACGGCGCCCGCTTCTTCGACCATGTCAGTAACGAACAAAGCGCCGCGCCGCG
CACCCTCGTGGTTCACGAGGTCATGGGCCGTCATTGCGGCTGGCTGACTGCAGCAACCGCGCGCGCCTACATGCAGATGG
CCGGCAACAAGGATTATGTCGACGGCTTCATGATGAACGCCCAGTTGAAGAATATCGACGGGCTCTATCTGCCGGAGATG
AAATTCGATCTCGAATCAGAAGCCGAGCGCCTGCGCGACGTAATGGATCGTGTCGGCTTCGTTACTCTGTTCGTCAGTGA
AGGTGCCTGCCTGGACGCCATTGTCGCCGAACGAGAGGCCGCCGGAGAGACGGTCAAGCGCGATGCTTTCGGTCACGTGA
AGATCGACACGATCAATGTCGGCAACTGGTTCTCCAAGCAGTTTGCAGCGCTCCTCGGCGCCGAGCGCTCCATGGTGCAG
AAGTCCGGCTACTATGCCCGATCGGCGCCCGCCAACGGCGACGATCTGCGCCTCATCCAGAGCATGGTCGATCTGGCGGT
CGAAAGTGCACTCAACAAGGTCTCCGGCGTGACCGGCCATGACGAGGACCAGGGAGGGCGGCTCCGTACGATCGAGTTTC
CGCGCATCAAGGGCGGAAAGCATTTCGACACATCCGTGAAGTGGTTCGGCGAGGTGATGAATGCCGTGGGCCAGAAATGG
CAGGCCGCCGACTGA

Upstream 100 bases:

>100_bases
TTGCGTTAGACCACCGTTATCTTTGATCGAAGGCAAGGATTGTCCGGACGCGATCGAATCCAGAAGCGATCGCCTCATGT
CGGAATCCGAAGGAGGACCC

Downstream 100 bases:

>100_bases
CGAATGCCGAACGGTTCGCTGTTTTCTGAAACGGCGAACCGCTTCCTGCCCGAATACGTTTAAGACAGCCGTTGACGGCG
GCGCGCGGGCATGGCTCCCT

Product: pyrophosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAKKKVAMLTAGGLAPCLSSAVGGLIGRYTDVAPDYELVAYRSGYQGLLLADRIEITPAMRERAHVLHRHGGSPIGNSRV
KLTNTADCVKRGLVKEGENPLRVAAERLASDGISILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIRQ
SLGAWTAAEYGARFFDHVSNEQSAAPRTLVVHEVMGRHCGWLTAATARAYMQMAGNKDYVDGFMMNAQLKNIDGLYLPEM
KFDLESEAERLRDVMDRVGFVTLFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGNWFSKQFAALLGAERSMVQ
KSGYYARSAPANGDDLRLIQSMVDLAVESALNKVSGVTGHDEDQGGRLRTIEFPRIKGGKHFDTSVKWFGEVMNAVGQKW
QAAD

Sequences:

>Translated_404_residues
MAKKKVAMLTAGGLAPCLSSAVGGLIGRYTDVAPDYELVAYRSGYQGLLLADRIEITPAMRERAHVLHRHGGSPIGNSRV
KLTNTADCVKRGLVKEGENPLRVAAERLASDGISILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIRQ
SLGAWTAAEYGARFFDHVSNEQSAAPRTLVVHEVMGRHCGWLTAATARAYMQMAGNKDYVDGFMMNAQLKNIDGLYLPEM
KFDLESEAERLRDVMDRVGFVTLFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGNWFSKQFAALLGAERSMVQ
KSGYYARSAPANGDDLRLIQSMVDLAVESALNKVSGVTGHDEDQGGRLRTIEFPRIKGGKHFDTSVKWFGEVMNAVGQKW
QAAD
>Mature_403_residues
AKKKVAMLTAGGLAPCLSSAVGGLIGRYTDVAPDYELVAYRSGYQGLLLADRIEITPAMRERAHVLHRHGGSPIGNSRVK
LTNTADCVKRGLVKEGENPLRVAAERLASDGISILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIRQS
LGAWTAAEYGARFFDHVSNEQSAAPRTLVVHEVMGRHCGWLTAATARAYMQMAGNKDYVDGFMMNAQLKNIDGLYLPEMK
FDLESEAERLRDVMDRVGFVTLFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGNWFSKQFAALLGAERSMVQK
SGYYARSAPANGDDLRLIQSMVDLAVESALNKVSGVTGHDEDQGGRLRTIEFPRIKGGKHFDTSVKWFGEVMNAVGQKWQ
AAD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family. PFP subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=220, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=218, Percent_Identity=32.1100917431193, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022953
- InterPro:   IPR000023
- InterPro:   IPR011405 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43726; Mature: 43595

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKVAMLTAGGLAPCLSSAVGGLIGRYTDVAPDYELVAYRSGYQGLLLADRIEITPAM
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCHHH
RERAHVLHRHGGSPIGNSRVKLTNTADCVKRGLVKEGENPLRVAAERLASDGISILHTIG
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECC
GDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIRQSLGAWTAAEYGARFFDHVSN
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCC
EQSAAPRTLVVHEVMGRHCGWLTAATARAYMQMAGNKDYVDGFMMNAQLKNIDGLYLPEM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCEECCCC
KFDLESEAERLRDVMDRVGFVTLFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINV
CCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEC
GNWFSKQFAALLGAERSMVQKSGYYARSAPANGDDLRLIQSMVDLAVESALNKVSGVTGH
CCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEDQGGRLRTIEFPRIKGGKHFDTSVKWFGEVMNAVGQKWQAAD
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKKKVAMLTAGGLAPCLSSAVGGLIGRYTDVAPDYELVAYRSGYQGLLLADRIEITPAM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCHHH
RERAHVLHRHGGSPIGNSRVKLTNTADCVKRGLVKEGENPLRVAAERLASDGISILHTIG
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECC
GDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIRQSLGAWTAAEYGARFFDHVSN
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCC
EQSAAPRTLVVHEVMGRHCGWLTAATARAYMQMAGNKDYVDGFMMNAQLKNIDGLYLPEM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCEECCCC
KFDLESEAERLRDVMDRVGFVTLFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINV
CCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEC
GNWFSKQFAALLGAERSMVQKSGYYARSAPANGDDLRLIQSMVDLAVESALNKVSGVTGH
CCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEDQGGRLRTIEFPRIKGGKHFDTSVKWFGEVMNAVGQKWQAAD
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653240; 1317210 [H]