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The map label for this gene is bigR [H]

Identifier: 150396818

GI number: 150396818

Start: 1703855

End: 1704187

Strand: Reverse

Name: bigR [H]

Synonym: Smed_1609

Alternate gene names: 150396818

Gene position: 1704187-1703855 (Counterclockwise)

Preceding gene: 150396819

Following gene: 150396817

Centisome position: 45.06

GC content: 59.76

Gene sequence:

>333_bases
ATGGGAACGATTACCAAATTCCGGAACATTCGCCCTTGCATGAGAGAGCGGGCCGACGAGGCCGCGGCACTGCTCAAGGC
GGTTGCAAACGAGAACCGGCTGATGATCCTGTGCACCCTTATCGAAGGCGAACACTCGGTCGGGCAGTTGGAAGAGATGC
TCGGTATCCGACAACCGTCCTTGTCGCAACAATTGACGGTGCTGCGAGAGGCCGGCATCGTGGCGACGCGACGCGACGCG
AAACAGATATTCTATCGGCTCGCCGAGGACAAAGCCGTGAGGCTGGTCACGGCGCTATATGAGATTTTCTGCCGCCAGGG
GGGCGGCCGATGA

Upstream 100 bases:

>100_bases
TTGCGGCGGCCGCCTGCCGGAACCGGAGGCCAACGGCGCCCGTTACCTGAAGTTTCCACTGAACGCGCTCAAGGGAGCGG
CGTGGGGCTGAGGTCCTGTC

Downstream 100 bases:

>100_bases
GCGGTCCCCCCGACCTCCGCTGGGCCTTTTCCGGCACCGGAAAAGGCCGCCGAGGCAGCTCCGTTGACAAAGTGTCAACC
TAAATTTGGATCGGACGGGC

Product: regulatory protein ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MGTITKFRNIRPCMRERADEAAALLKAVANENRLMILCTLIEGEHSVGQLEEMLGIRQPSLSQQLTVLREAGIVATRRDA
KQIFYRLAEDKAVRLVTALYEIFCRQGGGR

Sequences:

>Translated_110_residues
MGTITKFRNIRPCMRERADEAAALLKAVANENRLMILCTLIEGEHSVGQLEEMLGIRQPSLSQQLTVLREAGIVATRRDA
KQIFYRLAEDKAVRLVTALYEIFCRQGGGR
>Mature_109_residues
GTITKFRNIRPCMRERADEAAALLKAVANENRLMILCTLIEGEHSVGQLEEMLGIRQPSLSQQLTVLREAGIVATRRDAK
QIFYRLAEDKAVRLVTALYEIFCRQGGGR

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=92, Percent_Identity=32.6086956521739, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12391; Mature: 12260

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTITKFRNIRPCMRERADEAAALLKAVANENRLMILCTLIEGEHSVGQLEEMLGIRQPS
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCC
LSQQLTVLREAGIVATRRDAKQIFYRLAEDKAVRLVTALYEIFCRQGGGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTITKFRNIRPCMRERADEAAALLKAVANENRLMILCTLIEGEHSVGQLEEMLGIRQPS
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCC
LSQQLTVLREAGIVATRRDAKQIFYRLAEDKAVRLVTALYEIFCRQGGGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]