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The map label for this gene is trmL [H]

Identifier: 150396754

GI number: 150396754

Start: 1638184

End: 1638645

Strand: Direct

Name: trmL [H]

Synonym: Smed_1544

Alternate gene names: 150396754

Gene position: 1638184-1638645 (Clockwise)

Preceding gene: 150396748

Following gene: 150396756

Centisome position: 43.32

GC content: 61.04

Gene sequence:

>462_bases
ATGAACGAATTGCGCATCGCCCTCTACCAGCCCGACATCCCCGGCAATACCGGAACGATCCTGCGGCTCGCCGCCTGCCT
TGGCCTTGCGGTCGACATCATAGAGCCTGCCGGTTTTGATCTTTCCGACCGCAATCTCAAGCGCGCCGGGATGGACTATA
TCGCGGCAGTGACCATGACTCGGCACGTCAACTGGGAACGATTCGAAACATGGCGGTCGAGCACCGGCAGACGGCTTGTG
CTCGCCTCCACGAAAGCGGCAGTTCCCTATACGCGCTTCATGTTCAAACCGGACGATATCCTCCTCCTCGGCAGAGAAAG
CGCCGGCGTTCCTGATCAAGTCCATGAGCGGGCAGACGCACGCATCCTCATTCCAATGGCGCCCGGCCAGCGTTCGCTCA
ACATCGCCATGGCCGGCGCCATGATCGTGGGCGAAGCGATACGGCAGACAGACGCGCTCTGA

Upstream 100 bases:

>100_bases
ACCCCGGGACGAGCCGGTGTGGCCATGGATCGGAAGCCCATTCAGTGTCATAGAAACAGCCATTATAACGATCCACAGGT
TTCAATCCTCCGACAGGCCC

Downstream 100 bases:

>100_bases
GGCGGTACGCGAAACACGCGAAACACCCGGGCCGTGTTCGAACTTACCGAGCACTTCAGGCGGCGATCTCGTTATAGGGT
GTGGCCTTGCGGGCGTGTTT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNELRIALYQPDIPGNTGTILRLAACLGLAVDIIEPAGFDLSDRNLKRAGMDYIAAVTMTRHVNWERFETWRSSTGRRLV
LASTKAAVPYTRFMFKPDDILLLGRESAGVPDQVHERADARILIPMAPGQRSLNIAMAGAMIVGEAIRQTDAL

Sequences:

>Translated_153_residues
MNELRIALYQPDIPGNTGTILRLAACLGLAVDIIEPAGFDLSDRNLKRAGMDYIAAVTMTRHVNWERFETWRSSTGRRLV
LASTKAAVPYTRFMFKPDDILLLGRESAGVPDQVHERADARILIPMAPGQRSLNIAMAGAMIVGEAIRQTDAL
>Mature_153_residues
MNELRIALYQPDIPGNTGTILRLAACLGLAVDIIEPAGFDLSDRNLKRAGMDYIAAVTMTRHVNWERFETWRSSTGRRLV
LASTKAAVPYTRFMFKPDDILLLGRESAGVPDQVHERADARILIPMAPGQRSLNIAMAGAMIVGEAIRQTDAL

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=38.5135135135135, Blast_Score=106, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16850; Mature: 16850

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRIALYQPDIPGNTGTILRLAACLGLAVDIIEPAGFDLSDRNLKRAGMDYIAAVTMT
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHH
RHVNWERFETWRSSTGRRLVLASTKAAVPYTRFMFKPDDILLLGRESAGVPDQVHERADA
HCCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHCCCC
RILIPMAPGQRSLNIAMAGAMIVGEAIRQTDAL
EEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNELRIALYQPDIPGNTGTILRLAACLGLAVDIIEPAGFDLSDRNLKRAGMDYIAAVTMT
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHH
RHVNWERFETWRSSTGRRLVLASTKAAVPYTRFMFKPDDILLLGRESAGVPDQVHERADA
HCCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHCCCC
RILIPMAPGQRSLNIAMAGAMIVGEAIRQTDAL
EEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA