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The map label for this gene is norM [H]

Identifier: 150396481

GI number: 150396481

Start: 1356867

End: 1358270

Strand: Direct

Name: norM [H]

Synonym: Smed_1263

Alternate gene names: 150396481

Gene position: 1356867-1358270 (Clockwise)

Preceding gene: 150396480

Following gene: 150396482

Centisome position: 35.88

GC content: 61.32

Gene sequence:

>1404_bases
ATGCTGACTGCGACCAAAGATCTCGTGCTTCCGCAGGGCGACAATTCGTGGGCCACGCATTTTCGTGCGAGCCTTTATCT
GGGTGTGCCCTTCATCGGCGCGCAGCTCGCGCAGCTGGCGATCAATACCACGGACGTGCTCATGGTCGGTCAGTTGGGGG
CGACCCAGCTTGCATCGATCATCCTTGCGACCCAGGTCTTTTTCACGATCTTCATCTTCGGCAGCGGGTTTGCCAATGCA
GTGGTCCCGATGGTCGCTCAGGCGCAGGGGCGGGGCGACCATATCTCCGTCCGGCGTTCGGTGCGGATGGGCATGTGGGT
GGTGCTGCTTTACGGCGTACTGACGGCGCCGATCCTCTGGATGGCCGAGCCGATCCTCCTCTTTGCCGGACAGGAACCCG
AAGTCGCGGCACTTGCCGGGGAATATCTCCATATAGCCCAGTGGGCGATCTTCCCGAGCCTTCTGTTCATGGTGCTCAGA
GCATTCCTGAGCGGCCTCGAACGGGCGGGGGTGATCCTCTATGTAACGCTCGTGACCCTGGTTCTGAATGCCGCGCTTTG
TTACGTTCTCATTTTCGGCCATTTCGGCTTTCCGGAACTGGGGATCTTCGGCGCGGCCGTTGCCGCCCTTGGCGTAGCGC
TGCTCGGCGCGGCCCTGACGATCGGCTATATCCGCCGGCAACCGGAACTTCACCGCTATGAGCTTTTCGTCCGCTTCTGG
CGACCGGACTGGCCGGCCTTTGGCGAGGTGATCTATCTCGGCATTCCGATCTCGATTACAATTCTCGCCGAAGTGAGCCT
CTTTACCGTCGCCTCGCTGCTGATGGGCACGATCGGCACCATAGAACTGGCAGCCCATGGCATAGCGCTCCAGTTCGCCT
CCATCGCCTTCATGATCCCTCTTGGGCTGGCACAGGCCGGAACGGTGCGGATCGGCTTGGCCTATGGCAGCGGCGACATG
GTCGGCGTGAAGCGTGCCGCGGTTGCGGTGCTAACGCTCGGCATCGGCTTTGCTGCCGTCGGCAGCACGATTTTCGCGCT
GTTTCCGCATGATCTCGCGTCGCTCTATCTCGATACGAGGCGACCGGATGCTGCCGAAGTTCTCGCCTTTGCCGGACCGC
TGATCGTCATTGCCGGCGCCTTCCAGCTCGTAGATGGCCTTCAGGCGATCGCCGCTGGTATGCTGCGCGGGTTGAAGGAC
ACAACAGTGCCGATGATCCTGGCGATGATCGCCTATTGGCCGATAGGCTTCTTCTGCGCCTGGATCTTTGCTTTCCCGCT
GAATTTCGGCGGTGTCGGCGTGTGGTTCGGCTTCGTCCTTGGCCTTGCATCGGCGGCGCTCTTGCTCAACTGGCGCTTCT
TTCGACTGCTTCGATCGGTCAGCAGGGTGCCGGCTGCGTCTTAG

Upstream 100 bases:

>100_bases
GCGCCATTACCGCTGGCGCAAGTCCCGGTGGTGCTCTAATTCGATCGTTCTTCTTGCTTTACCTATTTTGTCCACTGCCG
CTGCAGAGCCAGGATTCTTC

Downstream 100 bases:

>100_bases
ATCATGATGTTTTCAGGTCGGATCGGCCGTGTGAGAAGCGTTTGGCGGTTCATGATCGCTTGAGGCAGACCTCTGCTCGA
GGCAGTCGCAATGCTGCATC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLTATKDLVLPQGDNSWATHFRASLYLGVPFIGAQLAQLAINTTDVLMVGQLGATQLASIILATQVFFTIFIFGSGFANA
VVPMVAQAQGRGDHISVRRSVRMGMWVVLLYGVLTAPILWMAEPILLFAGQEPEVAALAGEYLHIAQWAIFPSLLFMVLR
AFLSGLERAGVILYVTLVTLVLNAALCYVLIFGHFGFPELGIFGAAVAALGVALLGAALTIGYIRRQPELHRYELFVRFW
RPDWPAFGEVIYLGIPISITILAEVSLFTVASLLMGTIGTIELAAHGIALQFASIAFMIPLGLAQAGTVRIGLAYGSGDM
VGVKRAAVAVLTLGIGFAAVGSTIFALFPHDLASLYLDTRRPDAAEVLAFAGPLIVIAGAFQLVDGLQAIAAGMLRGLKD
TTVPMILAMIAYWPIGFFCAWIFAFPLNFGGVGVWFGFVLGLASAALLLNWRFFRLLRSVSRVPAAS

Sequences:

>Translated_467_residues
MLTATKDLVLPQGDNSWATHFRASLYLGVPFIGAQLAQLAINTTDVLMVGQLGATQLASIILATQVFFTIFIFGSGFANA
VVPMVAQAQGRGDHISVRRSVRMGMWVVLLYGVLTAPILWMAEPILLFAGQEPEVAALAGEYLHIAQWAIFPSLLFMVLR
AFLSGLERAGVILYVTLVTLVLNAALCYVLIFGHFGFPELGIFGAAVAALGVALLGAALTIGYIRRQPELHRYELFVRFW
RPDWPAFGEVIYLGIPISITILAEVSLFTVASLLMGTIGTIELAAHGIALQFASIAFMIPLGLAQAGTVRIGLAYGSGDM
VGVKRAAVAVLTLGIGFAAVGSTIFALFPHDLASLYLDTRRPDAAEVLAFAGPLIVIAGAFQLVDGLQAIAAGMLRGLKD
TTVPMILAMIAYWPIGFFCAWIFAFPLNFGGVGVWFGFVLGLASAALLLNWRFFRLLRSVSRVPAAS
>Mature_467_residues
MLTATKDLVLPQGDNSWATHFRASLYLGVPFIGAQLAQLAINTTDVLMVGQLGATQLASIILATQVFFTIFIFGSGFANA
VVPMVAQAQGRGDHISVRRSVRMGMWVVLLYGVLTAPILWMAEPILLFAGQEPEVAALAGEYLHIAQWAIFPSLLFMVLR
AFLSGLERAGVILYVTLVTLVLNAALCYVLIFGHFGFPELGIFGAAVAALGVALLGAALTIGYIRRQPELHRYELFVRFW
RPDWPAFGEVIYLGIPISITILAEVSLFTVASLLMGTIGTIELAAHGIALQFASIAFMIPLGLAQAGTVRIGLAYGSGDM
VGVKRAAVAVLTLGIGFAAVGSTIFALFPHDLASLYLDTRRPDAAEVLAFAGPLIVIAGAFQLVDGLQAIAAGMLRGLKD
TTVPMILAMIAYWPIGFFCAWIFAFPLNFGGVGVWFGFVLGLASAALLLNWRFFRLLRSVSRVPAAS

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=450, Percent_Identity=25.7777777777778, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI153792564, Length=428, Percent_Identity=23.3644859813084, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI38261960, Length=468, Percent_Identity=22.8632478632479, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=31.7286652078775, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI226510954, Length=439, Percent_Identity=25.5125284738041, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=416, Percent_Identity=25, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49954; Mature: 49954

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTATKDLVLPQGDNSWATHFRASLYLGVPFIGAQLAQLAINTTDVLMVGQLGATQLASI
CCCCCCCCEECCCCCCHHHHHHHHHEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
ILATQVFFTIFIFGSGFANAVVPMVAQAQGRGDHISVRRSVRMGMWVVLLYGVLTAPILW
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
MAEPILLFAGQEPEVAALAGEYLHIAQWAIFPSLLFMVLRAFLSGLERAGVILYVTLVTL
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VLNAALCYVLIFGHFGFPELGIFGAAVAALGVALLGAALTIGYIRRQPELHRYELFVRFW
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
RPDWPAFGEVIYLGIPISITILAEVSLFTVASLLMGTIGTIELAAHGIALQFASIAFMIP
CCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGLAQAGTVRIGLAYGSGDMVGVKRAAVAVLTLGIGFAAVGSTIFALFPHDLASLYLDTR
HCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAAEVLAFAGPLIVIAGAFQLVDGLQAIAAGMLRGLKDTTVPMILAMIAYWPIGFFCA
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
WIFAFPLNFGGVGVWFGFVLGLASAALLLNWRFFRLLRSVSRVPAAS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLTATKDLVLPQGDNSWATHFRASLYLGVPFIGAQLAQLAINTTDVLMVGQLGATQLASI
CCCCCCCCEECCCCCCHHHHHHHHHEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
ILATQVFFTIFIFGSGFANAVVPMVAQAQGRGDHISVRRSVRMGMWVVLLYGVLTAPILW
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
MAEPILLFAGQEPEVAALAGEYLHIAQWAIFPSLLFMVLRAFLSGLERAGVILYVTLVTL
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VLNAALCYVLIFGHFGFPELGIFGAAVAALGVALLGAALTIGYIRRQPELHRYELFVRFW
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
RPDWPAFGEVIYLGIPISITILAEVSLFTVASLLMGTIGTIELAAHGIALQFASIAFMIP
CCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGLAQAGTVRIGLAYGSGDMVGVKRAAVAVLTLGIGFAAVGSTIFALFPHDLASLYLDTR
HCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAAEVLAFAGPLIVIAGAFQLVDGLQAIAAGMLRGLKDTTVPMILAMIAYWPIGFFCA
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
WIFAFPLNFGGVGVWFGFVLGLASAALLLNWRFFRLLRSVSRVPAAS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]