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The map label for this gene is dat [H]

Identifier: 150396313

GI number: 150396313

Start: 1164541

End: 1165404

Strand: Direct

Name: dat [H]

Synonym: Smed_1094

Alternate gene names: 150396313

Gene position: 1164541-1165404 (Clockwise)

Preceding gene: 150396312

Following gene: 150396314

Centisome position: 30.79

GC content: 62.27

Gene sequence:

>864_bases
ATGCCGAGAATCGCCTATGTCAACGGCACTTATGTCCGGCACGCCGAGGCCGCCATTCACGTCGAGGATCGGGGCTATCA
GTTTGCCGACGGTGTCTACGAGGTCTGCGAGGTCCGGCACCGGTTCATCATCGACTTGAATCGGCATCTCGATCGCCTCG
GGCGATCGCTGCATGAGCTTGCGATAGGCTGGCCGATGAGTCGCGCGGCGCTGGTTCATGTCATAAGGGAGGTGCTGCGC
CGAAACAGGGTCCGCAACGGTCTCTTCTATCTCCAGGTGACCCGCGGCGTCGCGCGACGGGACCATGTGTTCCCCGCGGC
CGACACCCCGCCGTCGATCGTCGTAACCGCCAAGCGAACGGATCCGGGGGCGATCGCCCGCAAAAACACCGAGGGCATAT
CGGCGATAACCGTGCCGGAGAACCGGTGGGACCGGGTCGATATCAAGAGCATCGGCCTCCTGCCGAATGTGCTTGCCCGC
CAGCAGGCGAAGGAAGCGGGCGCGCAGGAGGCGATCTTCGTCGACGCGGACGGCATGGTCAAAGAGGGCGCGGCCACGAA
CGTATGGATCGTGGATCGTGAAGGGACACTGCGTACGCGTCCGGCGGAAAGCGGCATTTTGCGCGGCATTACCCGTACCA
CGCTCATGGACGTCGCCGGGCCGCTGGGTCTGACGGTGGAGGAGAGCGCTTTCTCCGTGGAGGAAATGCTCGCGGCGCGG
GAGGTCTTCATCACCGCCGCTACCAGCATCTGCTTTCCTGTCATTTCCGTCGACGGAAAGACGATCGGGAACGGTCATCC
AGGAAGCATAGCACAGAATATTCGCGAGGCCTTTTTCGACATTGCGGAAAAGACGTCGATTTGA

Upstream 100 bases:

>100_bases
AGGCCAAGGATCGCGTGGTACTGCTGGCGGCCGCCGAGACCGTCCGTCATGTCGAACAGCTCTTCCGCGTCAGCATTCAA
TATTTCTGAGGGACTCAGCC

Downstream 100 bases:

>100_bases
TACCAAACCTCTCGGAGGTGTCGGGGTTCGTTCGTGGCACCTTAAAAAATTGACAAAATGCACCGGTGAAAGAACGCCGG
CAAATAAGAAAGAAGCGGCG

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPRIAYVNGTYVRHAEAAIHVEDRGYQFADGVYEVCEVRHRFIIDLNRHLDRLGRSLHELAIGWPMSRAALVHVIREVLR
RNRVRNGLFYLQVTRGVARRDHVFPAADTPPSIVVTAKRTDPGAIARKNTEGISAITVPENRWDRVDIKSIGLLPNVLAR
QQAKEAGAQEAIFVDADGMVKEGAATNVWIVDREGTLRTRPAESGILRGITRTTLMDVAGPLGLTVEESAFSVEEMLAAR
EVFITAATSICFPVISVDGKTIGNGHPGSIAQNIREAFFDIAEKTSI

Sequences:

>Translated_287_residues
MPRIAYVNGTYVRHAEAAIHVEDRGYQFADGVYEVCEVRHRFIIDLNRHLDRLGRSLHELAIGWPMSRAALVHVIREVLR
RNRVRNGLFYLQVTRGVARRDHVFPAADTPPSIVVTAKRTDPGAIARKNTEGISAITVPENRWDRVDIKSIGLLPNVLAR
QQAKEAGAQEAIFVDADGMVKEGAATNVWIVDREGTLRTRPAESGILRGITRTTLMDVAGPLGLTVEESAFSVEEMLAAR
EVFITAATSICFPVISVDGKTIGNGHPGSIAQNIREAFFDIAEKTSI
>Mature_286_residues
PRIAYVNGTYVRHAEAAIHVEDRGYQFADGVYEVCEVRHRFIIDLNRHLDRLGRSLHELAIGWPMSRAALVHVIREVLRR
NRVRNGLFYLQVTRGVARRDHVFPAADTPPSIVVTAKRTDPGAIARKNTEGISAITVPENRWDRVDIKSIGLLPNVLARQ
QAKEAGAQEAIFVDADGMVKEGAATNVWIVDREGTLRTRPAESGILRGITRTTLMDVAGPLGLTVEESAFSVEEMLAARE
VFITAATSICFPVISVDGKTIGNGHPGSIAQNIREAFFDIAEKTSI

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=295, Percent_Identity=29.4915254237288, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787338, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31629; Mature: 31497

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIAYVNGTYVRHAEAAIHVEDRGYQFADGVYEVCEVRHRFIIDLNRHLDRLGRSLHEL
CCEEEEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
AIGWPMSRAALVHVIREVLRRNRVRNGLFYLQVTRGVARRDHVFPAADTPPSIVVTAKRT
HHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCCCCCCCCCCEEEEEECC
DPGAIARKNTEGISAITVPENRWDRVDIKSIGLLPNVLARQQAKEAGAQEAIFVDADGMV
CCCCCEECCCCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCC
KEGAATNVWIVDREGTLRTRPAESGILRGITRTTLMDVAGPLGLTVEESAFSVEEMLAAR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHH
EVFITAATSICFPVISVDGKTIGNGHPGSIAQNIREAFFDIAEKTSI
HHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRIAYVNGTYVRHAEAAIHVEDRGYQFADGVYEVCEVRHRFIIDLNRHLDRLGRSLHEL
CEEEEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
AIGWPMSRAALVHVIREVLRRNRVRNGLFYLQVTRGVARRDHVFPAADTPPSIVVTAKRT
HHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCCCCCCCCCCEEEEEECC
DPGAIARKNTEGISAITVPENRWDRVDIKSIGLLPNVLARQQAKEAGAQEAIFVDADGMV
CCCCCEECCCCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCC
KEGAATNVWIVDREGTLRTRPAESGILRGITRTTLMDVAGPLGLTVEESAFSVEEMLAAR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHH
EVFITAATSICFPVISVDGKTIGNGHPGSIAQNIREAFFDIAEKTSI
HHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]