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The map label for this gene is ntrX [H]

Identifier: 150396311

GI number: 150396311

Start: 1161724

End: 1163088

Strand: Direct

Name: ntrX [H]

Synonym: Smed_1092

Alternate gene names: 150396311

Gene position: 1161724-1163088 (Clockwise)

Preceding gene: 150396310

Following gene: 150396312

Centisome position: 30.72

GC content: 61.17

Gene sequence:

>1365_bases
ATGGCGGCTGATATTCTGGTTGTAGATGACGAGGAGGACATTCGCGAGATCGTCTCGGGAATCCTCTCTGACGAAGGCCA
TGAGACGCGCACCGCCTTCGACAGCGAGAGTGCGCTTGCGGCGATCAACGACCGCCTGCCGCGTCTGATCTTCCTCGACA
TCTGGATGCAGGGGAGCAAGCTCGACGGACTGGCGCTCCTGGACGAGATCAAGAGCCGCCACCCCGATTTGCCGGTGGTG
ATGATTTCAGGCCACGGCAACATCGAAACGGCCGTTTCGGCGATCAAGCGCGGCGCCTATGACTTCATCGAGAAACCGTT
CAAGGCCGACCGGCTGATCCTTATCGCCGAGCGCGCGCTCGAAAATTCCAAGCTCAAGCGCGAAAACTTCGAATTGAAGA
GGAAGTCGGGCGATCCGGTGGAACTCATCGGAACCTCCGTCGCGGTCTCGCAGCTGCGCCAGATGATCGAGAAGGTGGCG
CCGACCAATAGCCGCATCATGATTCAGGGACCGTCCGGCTCCGGCAAGGAACTCGTTGCGCGGATGATTCATCGCAAATC
CGCACGCGCCAACGGTCCTTTTGTCGCCCTCAATGCGGCTGCGATCACGCCGGACAAAATGGAGATCGCGCTGTTCGGCA
CCGAGGGTACGACCGGACAACCGCGCCGGACCGGAGCGCTCGAGGAAGCTCATGGCGGTATTCTTTACCTCGATGAAGTC
GGCGAAATGCCACGCGAGACGCAGAACAAGATCCTGCGCGTCCTCGTCGATCAGCAGTTCGAGCGGGTAGGCGGGTCCAA
GCGCGTCAAGGTCGATGTCCGCATCATCTCCTCGACCGCCTACAATCTCGAGAACATGATCGCCGAGGGCCTGTTCCGCG
AGGATCTCTACCACCGGCTCGCCGTTATTCCGGTGCGCGTTCCTGCTCTTGCGGAGCGCCGCGAGGATATTCCTTTCCTG
GTCGACATGTTCATGCGCCAGGTCAGCGAGCAGGCCGGCATCCGACCCCGCAAGATAGGCGAGGATGCGCTCGCCGTACT
GCAGGCGCATGACTGGCCCGGCAATATCCGGCAGTTGCGCAACAATATCGAGCGCCTGATGATCCTGGCGCGTAGCGACG
GTCCGGACACGCCGATCACCGCCGACATGCTGCCGAACGAGGTTGGAGACACCCTGCCGAAGATTTCGGCGCAAAGCGAT
CAGCACATCATGACCCTGCCGCTGAGGGAGGCGCGCGAGATGTTCGAGCGCGACTATCTGATCGCCCAGATCAACCGCTT
CGGCGGCAACATCTCGCGCACAGCCGAATTCGTCGGCATGGAACGCTCCGCTCTTCATCGCAAGCTGAAGTCGCTCGGCG
TGTGA

Upstream 100 bases:

>100_bases
TCGGATTTCGACGGCGGCGCCGGGGCGATGATCCGGGTGATCCTGCCGCCTGCCGGCGAGACCGGCGGTGAACATAATTT
GAAGGATAAGGGTAATTTCA

Downstream 100 bases:

>100_bases
TTATCCCGGTCGTGAATTCAATTTTCGGGCCAGCCGCAGAACCGGCCAGACATAAGGAACAGCCATGAAGGTGATCATAT
GCGGGGCAGGTCAGGTCGGC

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAADILVVDDEEDIREIVSGILSDEGHETRTAFDSESALAAINDRLPRLIFLDIWMQGSKLDGLALLDEIKSRHPDLPVV
MISGHGNIETAVSAIKRGAYDFIEKPFKADRLILIAERALENSKLKRENFELKRKSGDPVELIGTSVAVSQLRQMIEKVA
PTNSRIMIQGPSGSGKELVARMIHRKSARANGPFVALNAAAITPDKMEIALFGTEGTTGQPRRTGALEEAHGGILYLDEV
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMIAEGLFREDLYHRLAVIPVRVPALAERREDIPFL
VDMFMRQVSEQAGIRPRKIGEDALAVLQAHDWPGNIRQLRNNIERLMILARSDGPDTPITADMLPNEVGDTLPKISAQSD
QHIMTLPLREAREMFERDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Sequences:

>Translated_454_residues
MAADILVVDDEEDIREIVSGILSDEGHETRTAFDSESALAAINDRLPRLIFLDIWMQGSKLDGLALLDEIKSRHPDLPVV
MISGHGNIETAVSAIKRGAYDFIEKPFKADRLILIAERALENSKLKRENFELKRKSGDPVELIGTSVAVSQLRQMIEKVA
PTNSRIMIQGPSGSGKELVARMIHRKSARANGPFVALNAAAITPDKMEIALFGTEGTTGQPRRTGALEEAHGGILYLDEV
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMIAEGLFREDLYHRLAVIPVRVPALAERREDIPFL
VDMFMRQVSEQAGIRPRKIGEDALAVLQAHDWPGNIRQLRNNIERLMILARSDGPDTPITADMLPNEVGDTLPKISAQSD
QHIMTLPLREAREMFERDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
>Mature_453_residues
AADILVVDDEEDIREIVSGILSDEGHETRTAFDSESALAAINDRLPRLIFLDIWMQGSKLDGLALLDEIKSRHPDLPVVM
ISGHGNIETAVSAIKRGAYDFIEKPFKADRLILIAERALENSKLKRENFELKRKSGDPVELIGTSVAVSQLRQMIEKVAP
TNSRIMIQGPSGSGKELVARMIHRKSARANGPFVALNAAAITPDKMEIALFGTEGTTGQPRRTGALEEAHGGILYLDEVG
EMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMIAEGLFREDLYHRLAVIPVRVPALAERREDIPFLV
DMFMRQVSEQAGIRPRKIGEDALAVLQAHDWPGNIRQLRNNIERLMILARSDGPDTPITADMLPNEVGDTLPKISAQSDQ
HIMTLPLREAREMFERDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.2478632478632, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=36.8421052631579, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=35.5555555555556, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=33.5555555555556, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=36.9905956112853, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=36.5930599369085, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=36.6666666666667, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=33.5276967930029, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=35.8255451713396, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1787583, Length=316, Percent_Identity=34.8101265822785, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=34.2857142857143, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI87081858, Length=307, Percent_Identity=28.3387622149837, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=33.1983805668016, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=38.2608695652174, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50603; Mature: 50472

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADILVVDDEEDIREIVSGILSDEGHETRTAFDSESALAAINDRLPRLIFLDIWMQGSK
CCCCEEEECCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCC
LDGLALLDEIKSRHPDLPVVMISGHGNIETAVSAIKRGAYDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEEHHHH
ENSKLKRENFELKRKSGDPVELIGTSVAVSQLRQMIEKVAPTNSRIMIQGPSGSGKELVA
HCCHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
RMIHRKSARANGPFVALNAAAITPDKMEIALFGTEGTTGQPRRTGALEEAHGGILYLDEV
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMIAEGLFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVIPVRVPALAERREDIPFLVDMFMRQVSEQAGIRPRKIGEDALAVLQAHDWPGNIRQLR
HHEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARSDGPDTPITADMLPNEVGDTLPKISAQSDQHIMTLPLREAREMFERDYL
HHHHHEEEEECCCCCCCCEEHHCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHH
IAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AADILVVDDEEDIREIVSGILSDEGHETRTAFDSESALAAINDRLPRLIFLDIWMQGSK
CCCEEEECCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCC
LDGLALLDEIKSRHPDLPVVMISGHGNIETAVSAIKRGAYDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEEHHHH
ENSKLKRENFELKRKSGDPVELIGTSVAVSQLRQMIEKVAPTNSRIMIQGPSGSGKELVA
HCCHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
RMIHRKSARANGPFVALNAAAITPDKMEIALFGTEGTTGQPRRTGALEEAHGGILYLDEV
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMIAEGLFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVIPVRVPALAERREDIPFLVDMFMRQVSEQAGIRPRKIGEDALAVLQAHDWPGNIRQLR
HHEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARSDGPDTPITADMLPNEVGDTLPKISAQSDQHIMTLPLREAREMFERDYL
HHHHHEEEEECCCCCCCCEEHHCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHH
IAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]