Click here to switch to the map view.

The map label for this gene is lpd [H]

Identifier: 150396301

GI number: 150396301

Start: 1149634

End: 1151079

Strand: Direct

Name: lpd [H]

Synonym: Smed_1081

Alternate gene names: 150396301

Gene position: 1149634-1151079 (Clockwise)

Preceding gene: 150396300

Following gene: 150396302

Centisome position: 30.4

GC content: 62.31

Gene sequence:

>1446_bases
ATGGCTGAGAATTACGACGTGATCGTTGTCGGTTCGGGTCCGGGCGGCTACGTTACCGCCATCCGCTCGGCACAACTTGG
CTTGAAGACGGCGATCGTCGAGCGCGAACATCTCGGCGGCATCTGCCTCAATTGGGGCTGCATTCCGACCAAGGCGCTTC
TACGTTCCGCCGAAATCCTCGACCATGCCAATCATGCCAAGAACTACGGCCTGACGCTCGAAGGCAAGATCACCGCCAAC
GTCAAGGATGTCGTCGCCCGGTCGCGGGCGGTCTCGGCACGCTTGAACGGCGGCGTCGCCTTCCTGATGAAGAAGAACAA
GGTCGACGTGATCTGGGGCGAGGCGAAGCTGACGAAGCCCGGCGAAATCGTCGTAGGCAGCCCGTCCAGGCCGGCTGTCC
AGCCGCAGAATCCGGTACCGAAGGGCGTCAAGGGCGAGGGCACCTATACCGCTAAACACATCATCCTCGCCACCGGCGCC
CGCCCGCGGGCACTTCCCGGCATCGAGCCGGATGGGAAGCTGATCTGGACCTATTTCGAGGCGATGAAGCCGGCGGAATT
TCCGAAATCGCTGCTCGTGATGGGCTCCGGTGCGATCGGGATCGAATTCGCGAGCTTCTACCGCTCGATGGGCGTCGACG
TGACAGTGATCGAACTGCTGCCGCAGATCATGCCGGTCGAGGATGCGGAGATCTCAGCCTTCGCTCGCAAGCAGCTCGAA
AAACGCGGCCTGAAGATCATCACCGACGCCAAGGTGACAAAGGTCGAGAAGGGCGCCAACGACGTTACCGCGCATGTCGA
GACGAAGGACGGTAAGGTTACGCCGATGAAGGCCGAGCGCCTGATCTCCGCGGTAGGGGTGCAGGGCAACATCGAGAACC
TCGGCCTCGAGGCTCTTGGCATCAAGACCGACCGGGGCTGCATCGTTACCGATGGCTACGGCAAAACCAATGTGCCCGGC
ATCTATGCGATCGGCGACGTTGCCGGCCCGCCGATGCTGGCGCACAAGGCAGAACACGAGGGCGTGATCTGCGTCGAGAA
GATTGCAGGGGTCCCCGGCGTGCACGCACTCGACAAGGGCAAGATTCCGGGCTGCACCTATTGCGACCCCCAGGTCGCCT
CCGTCGGCCTCACGGAGGCCAGGGCCAAGGAACTCGGCCGCGACGTTCGCGTCGGCCGCTACAGCTTCAACGCGAACGGC
AAAGCGATCGCGCTCGGCGAGGACCAGGGCCTGATCAAGACGATCTTCGACAAGATGACCGGAGAATTGCTCGGCGCCCA
TATGGTGGGCGCCGAAGTCACCGAACTCATTCAGGGCTTCGTCGTCGCCATGAACCTGGAGACGACGGAAGAGGAGCTGA
TGCACACGGTGTTCCCGCACCCGACGCTTTCGGAGATGATGAAGGAAAGCGTGCTCGACGCCTACGGTCGGGTATTGAAC
GCCTGA

Upstream 100 bases:

>100_bases
AGTGGCGCGCGCCGCTGCGGTTCCACTCTTCGAAGACAGCAGGAAACACGACTGGGTGCTTTTCACCCGCAACGTCGCTT
GACGCAAAGGTAGGAAAAAC

Downstream 100 bases:

>100_bases
TGCCGTCGGCCGCCCGGAACATTTTGCGCTTTTCACGCATTGATTGTAGGATGCAGTTTCCACAAAGGGGAGAATCGAAT
GAGCTTGAATGGCGTGGGTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAENYDVIVVGSGPGGYVTAIRSAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILDHANHAKNYGLTLEGKITAN
VKDVVARSRAVSARLNGGVAFLMKKNKVDVIWGEAKLTKPGEIVVGSPSRPAVQPQNPVPKGVKGEGTYTAKHIILATGA
RPRALPGIEPDGKLIWTYFEAMKPAEFPKSLLVMGSGAIGIEFASFYRSMGVDVTVIELLPQIMPVEDAEISAFARKQLE
KRGLKIITDAKVTKVEKGANDVTAHVETKDGKVTPMKAERLISAVGVQGNIENLGLEALGIKTDRGCIVTDGYGKTNVPG
IYAIGDVAGPPMLAHKAEHEGVICVEKIAGVPGVHALDKGKIPGCTYCDPQVASVGLTEARAKELGRDVRVGRYSFNANG
KAIALGEDQGLIKTIFDKMTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTVFPHPTLSEMMKESVLDAYGRVLN
A

Sequences:

>Translated_481_residues
MAENYDVIVVGSGPGGYVTAIRSAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILDHANHAKNYGLTLEGKITAN
VKDVVARSRAVSARLNGGVAFLMKKNKVDVIWGEAKLTKPGEIVVGSPSRPAVQPQNPVPKGVKGEGTYTAKHIILATGA
RPRALPGIEPDGKLIWTYFEAMKPAEFPKSLLVMGSGAIGIEFASFYRSMGVDVTVIELLPQIMPVEDAEISAFARKQLE
KRGLKIITDAKVTKVEKGANDVTAHVETKDGKVTPMKAERLISAVGVQGNIENLGLEALGIKTDRGCIVTDGYGKTNVPG
IYAIGDVAGPPMLAHKAEHEGVICVEKIAGVPGVHALDKGKIPGCTYCDPQVASVGLTEARAKELGRDVRVGRYSFNANG
KAIALGEDQGLIKTIFDKMTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTVFPHPTLSEMMKESVLDAYGRVLN
A
>Mature_480_residues
AENYDVIVVGSGPGGYVTAIRSAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILDHANHAKNYGLTLEGKITANV
KDVVARSRAVSARLNGGVAFLMKKNKVDVIWGEAKLTKPGEIVVGSPSRPAVQPQNPVPKGVKGEGTYTAKHIILATGAR
PRALPGIEPDGKLIWTYFEAMKPAEFPKSLLVMGSGAIGIEFASFYRSMGVDVTVIELLPQIMPVEDAEISAFARKQLEK
RGLKIITDAKVTKVEKGANDVTAHVETKDGKVTPMKAERLISAVGVQGNIENLGLEALGIKTDRGCIVTDGYGKTNVPGI
YAIGDVAGPPMLAHKAEHEGVICVEKIAGVPGVHALDKGKIPGCTYCDPQVASVGLTEARAKELGRDVRVGRYSFNANGK
AIALGEDQGLIKTIFDKMTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTVFPHPTLSEMMKESVLDAYGRVLNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=485, Percent_Identity=36.701030927835, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI50301238, Length=480, Percent_Identity=29.5833333333333, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI22035672, Length=486, Percent_Identity=28.1893004115226, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI291045266, Length=494, Percent_Identity=26.1133603238866, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=24.8394004282655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=24.8394004282655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=24.8394004282655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=24.8394004282655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=24.8394004282655, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI291045268, Length=490, Percent_Identity=25.3061224489796, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=36.0086767895879, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082354, Length=485, Percent_Identity=27.8350515463918, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789915, Length=476, Percent_Identity=31.3025210084034, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=26.1702127659574, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=498, Percent_Identity=36.7469879518072, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=28.2327586206897, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=28.2327586206897, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=24.3852459016393, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=500, Percent_Identity=26.4, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=36.2886597938144, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6325166, Length=482, Percent_Identity=29.045643153527, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=488, Percent_Identity=27.6639344262295, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21358499, Length=482, Percent_Identity=38.1742738589212, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=26.694045174538, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=26.1316872427983, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=26.7465069860279, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17737741, Length=505, Percent_Identity=25.7425742574257, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51216; Mature: 51085

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENYDVIVVGSGPGGYVTAIRSAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEIL
CCCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DHANHAKNYGLTLEGKITANVKDVVARSRAVSARLNGGVAFLMKKNKVDVIWGEAKLTKP
HHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHEECCCCEEEEEECCCEEEEECCEEECCC
GEIVVGSPSRPAVQPQNPVPKGVKGEGTYTAKHIILATGARPRALPGIEPDGKLIWTYFE
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
AMKPAEFPKSLLVMGSGAIGIEFASFYRSMGVDVTVIELLPQIMPVEDAEISAFARKQLE
HCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHH
KRGLKIITDAKVTKVEKGANDVTAHVETKDGKVTPMKAERLISAVGVQGNIENLGLEALG
HCCCEEEECCHHHHECCCCCCEEEEEECCCCEECCHHHHHHHHHHCCCCCHHHCCEEEEE
IKTDRGCIVTDGYGKTNVPGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGVPGVHALDKG
EECCCCEEEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
KIPGCTYCDPQVASVGLTEARAKELGRDVRVGRYSFNANGKAIALGEDQGLIKTIFDKMT
CCCCCCCCCCHHHHCCCHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHH
GELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTVFPHPTLSEMMKESVLDAYGRVLN
HHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
AENYDVIVVGSGPGGYVTAIRSAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEIL
CCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DHANHAKNYGLTLEGKITANVKDVVARSRAVSARLNGGVAFLMKKNKVDVIWGEAKLTKP
HHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHEECCCCEEEEEECCCEEEEECCEEECCC
GEIVVGSPSRPAVQPQNPVPKGVKGEGTYTAKHIILATGARPRALPGIEPDGKLIWTYFE
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
AMKPAEFPKSLLVMGSGAIGIEFASFYRSMGVDVTVIELLPQIMPVEDAEISAFARKQLE
HCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHH
KRGLKIITDAKVTKVEKGANDVTAHVETKDGKVTPMKAERLISAVGVQGNIENLGLEALG
HCCCEEEECCHHHHECCCCCCEEEEEECCCCEECCHHHHHHHHHHCCCCCHHHCCEEEEE
IKTDRGCIVTDGYGKTNVPGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGVPGVHALDKG
EECCCCEEEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
KIPGCTYCDPQVASVGLTEARAKELGRDVRVGRYSFNANGKAIALGEDQGLIKTIFDKMT
CCCCCCCCCCHHHHCCCHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHH
GELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTVFPHPTLSEMMKESVLDAYGRVLN
HHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]