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The map label for this gene is yagI [H]

Identifier: 150395664

GI number: 150395664

Start: 475534

End: 476394

Strand: Direct

Name: yagI [H]

Synonym: Smed_0439

Alternate gene names: 150395664

Gene position: 475534-476394 (Clockwise)

Preceding gene: 150395657

Following gene: 150395665

Centisome position: 12.57

GC content: 62.6

Gene sequence:

>861_bases
ATGAGAATGAAGCGGACCAAAGAAGGCAGCATGGGCCGGGATACCGAAGGCACCGGCACGCTTGGCAAGGCAATGGCCGT
GCTGGAGGTCGTGGCCATGGCCGAACGCCCTCAGCGTTTCACGGATATATTAATGGCGCTCCGCCAGCCCCGGGGCACCC
TGCACAGGCTACTGGGCCATCTCGTCGAAGAGGGCTTGCTGGTGCAGCGTCCCGATTTGTCCTACGAGCCGGGGCTTCGC
CTGTTGAAATTCGCTTATCGCTCGTGGTCCGGCAATCGTTTCCGCGAGATTGCCGCCCCTTACCTCCTCAAGCTGCACCA
ACTGACCGGCGAGACCGTGCATCTGGGCGTGCTGCGGGAAACGGAGATCATCTATGTCGACAAGGTGGAGAGCCAGCAGA
CCGTGCGCATGAGCTCGCAGATCGGCAAGGCGTCGCCGGCCTATTGTACCGGTATAGGCAAGGCCGCTCTGTCCGTGCTG
CCTTCAAGTGAATTGCAGCGGATTTCGGCGCGAATGGAGTTCCGTCCGTTCACGCCGAAGACGCATCGCTCGGCCGAAAC
GCTCTATGCCGAGATCGCGGAAATTCGGCATGACGGCCATGCATTCGACCGAGAGGAGCACGAGGCGGAAATCTGCTGCG
TGGCCGCCCCGATAAGCGTTCCCGACCACGATCTGATCGGGGGGATCTCGGTGACCGGCCCTTCCTATCGCGTCAGCTTG
GCGCAGCTGACCGCCTGGGCGCAGGATGTGCGAAAGGCTGCGGCGGATATCGAGGAGGAGCTGCGGATAAGATCAGGGCC
TGGGCGGGAGACCGGCGGTGAAACGCCACGCGTCAGGTCAGACGCGCAAAGGCCGCGTTGA

Upstream 100 bases:

>100_bases
CCGTCAGGCAGGCTTGTTCCAGTATATGGAACTATGTTTTACTATCTGGAATTATCGCGCCGATGCTTCTATCCTGTCAA
GGTAGCGCAGCCGAGAGCGG

Downstream 100 bases:

>100_bases
CGCTTCAAGAATGGCCGCGTGTTTGATTCCGCGTGAACACCATGTAGACAGCAGAGGCCTAAAGACGTGGAGCAGAATCG
CTTTACGGTAAATTAGAAGT

Product: transcriptional regulator IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRMKRTKEGSMGRDTEGTGTLGKAMAVLEVVAMAERPQRFTDILMALRQPRGTLHRLLGHLVEEGLLVQRPDLSYEPGLR
LLKFAYRSWSGNRFREIAAPYLLKLHQLTGETVHLGVLRETEIIYVDKVESQQTVRMSSQIGKASPAYCTGIGKAALSVL
PSSELQRISARMEFRPFTPKTHRSAETLYAEIAEIRHDGHAFDREEHEAEICCVAAPISVPDHDLIGGISVTGPSYRVSL
AQLTAWAQDVRKAAADIEEELRIRSGPGRETGGETPRVRSDAQRPR

Sequences:

>Translated_286_residues
MRMKRTKEGSMGRDTEGTGTLGKAMAVLEVVAMAERPQRFTDILMALRQPRGTLHRLLGHLVEEGLLVQRPDLSYEPGLR
LLKFAYRSWSGNRFREIAAPYLLKLHQLTGETVHLGVLRETEIIYVDKVESQQTVRMSSQIGKASPAYCTGIGKAALSVL
PSSELQRISARMEFRPFTPKTHRSAETLYAEIAEIRHDGHAFDREEHEAEICCVAAPISVPDHDLIGGISVTGPSYRVSL
AQLTAWAQDVRKAAADIEEELRIRSGPGRETGGETPRVRSDAQRPR
>Mature_286_residues
MRMKRTKEGSMGRDTEGTGTLGKAMAVLEVVAMAERPQRFTDILMALRQPRGTLHRLLGHLVEEGLLVQRPDLSYEPGLR
LLKFAYRSWSGNRFREIAAPYLLKLHQLTGETVHLGVLRETEIIYVDKVESQQTVRMSSQIGKASPAYCTGIGKAALSVL
PSSELQRISARMEFRPFTPKTHRSAETLYAEIAEIRHDGHAFDREEHEAEICCVAAPISVPDHDLIGGISVTGPSYRVSL
AQLTAWAQDVRKAAADIEEELRIRSGPGRETGGETPRVRSDAQRPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=232, Percent_Identity=29.7413793103448, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786715, Length=253, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788131, Length=260, Percent_Identity=26.5384615384615, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788581, Length=219, Percent_Identity=30.5936073059361, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082359, Length=241, Percent_Identity=28.2157676348548, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790752, Length=251, Percent_Identity=24.7011952191235, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789999, Length=264, Percent_Identity=25.7575757575758, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31837; Mature: 31837

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKRTKEGSMGRDTEGTGTLGKAMAVLEVVAMAERPQRFTDILMALRQPRGTLHRLLGH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
LVEEGLLVQRPDLSYEPGLRLLKFAYRSWSGNRFREIAAPYLLKLHQLTGETVHLGVLRE
HHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEC
TEIIYVDKVESQQTVRMSSQIGKASPAYCTGIGKAALSVLPSSELQRISARMEFRPFTPK
CEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
THRSAETLYAEIAEIRHDGHAFDREEHEAEICCVAAPISVPDHDLIGGISVTGPSYRVSL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCEEECCCCHHHHH
AQLTAWAQDVRKAAADIEEELRIRSGPGRETGGETPRVRSDAQRPR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRMKRTKEGSMGRDTEGTGTLGKAMAVLEVVAMAERPQRFTDILMALRQPRGTLHRLLGH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
LVEEGLLVQRPDLSYEPGLRLLKFAYRSWSGNRFREIAAPYLLKLHQLTGETVHLGVLRE
HHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEC
TEIIYVDKVESQQTVRMSSQIGKASPAYCTGIGKAALSVLPSSELQRISARMEFRPFTPK
CEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
THRSAETLYAEIAEIRHDGHAFDREEHEAEICCVAAPISVPDHDLIGGISVTGPSYRVSL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCEEECCCCHHHHH
AQLTAWAQDVRKAAADIEEELRIRSGPGRETGGETPRVRSDAQRPR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]