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The map label for this gene is rlmE [H]

Identifier: 150395598

GI number: 150395598

Start: 399655

End: 400392

Strand: Direct

Name: rlmE [H]

Synonym: Smed_0373

Alternate gene names: 150395598

Gene position: 399655-400392 (Clockwise)

Preceding gene: 150395597

Following gene: 150395599

Centisome position: 10.57

GC content: 63.55

Gene sequence:

>738_bases
ATGACGAAACCCCCGGTCGGCAGCAACCGCAGCGGCCGAAAGCTCGGTCAGAAGGTCAAGAAGGGCAAGCTCAAGGCATC
CTCGCGCCGCTGGATCGAGCGCCACATCAACGATCCCTATGTGCAGCGCGCGCAGCTTGAAGGCTATCGGGCGCGGGCGG
CCTTCAAGCTTCTGGAGATAGACGAGAAGCACAAGATTCTCGCGGGCGCCAGGCGCATCATCGATCTGGGCGCCGCACCC
GGAAGCTGGTCCCAGATCGCCGCCAAGGTGACGAACTCGACCGATACCGATCCGCGCGTGGCTGCGATCGACTTCCTCGA
AATGGACGCCATTCCGGGCGTGAGCTTCCTGCAGATGGATTTCCTCGACCCGCAGGCGCCGGAAAAGCTGAAGGAGGCGA
TCGGCGGAGCGCCTGATATCGTCCTGTCCGACATGGCTGCGCCGACCACCGGTCACCGCCAGACCGACCATATCAGAACC
ATGCATCTCTGCGAGGTGGCGGCGCATTTCGCCGTCGAGGTTCTGGCGGAAGGCGGCCATTTTCTGGCGAAGACCTTCCA
GGGCGGCACCGAGCGCGACCTCCTGAACATGCTGAAGCAGAACTTCCGCCAGGTGGTCCATGTGAAGCCGGCGTCCTCAC
GCGCCGAATCGGTCGAGATGTTCCTGCTCGCCAAGGGCTTCAAAGGCCGCCACGCGTCAAGGAGCGACGAACCGGCAGAG
GGCGCCGCGGAGAAATAA

Upstream 100 bases:

>100_bases
ACGGGGCCTGGCGCCGGGCGCGTCCACGCCGCCACCAACGCAGCTTCGGTCCCCCGCAGGCGGGGGAGGAGTGCGCGAAG
CGCCATGAAGGAACCGACGC

Downstream 100 bases:

>100_bases
GCCATGCTGTTATACGTGACGCTCGGTTCCAACGACCTGGAAAGAGCAGGCGCCTTCTACGATGCCGCGCTTGCGCCCCT
CGGCCTCAAGCGCCGCAAAC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTKPPVGSNRSGRKLGQKVKKGKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEIDEKHKILAGARRIIDLGAAP
GSWSQIAAKVTNSTDTDPRVAAIDFLEMDAIPGVSFLQMDFLDPQAPEKLKEAIGGAPDIVLSDMAAPTTGHRQTDHIRT
MHLCEVAAHFAVEVLAEGGHFLAKTFQGGTERDLLNMLKQNFRQVVHVKPASSRAESVEMFLLAKGFKGRHASRSDEPAE
GAAEK

Sequences:

>Translated_245_residues
MTKPPVGSNRSGRKLGQKVKKGKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEIDEKHKILAGARRIIDLGAAP
GSWSQIAAKVTNSTDTDPRVAAIDFLEMDAIPGVSFLQMDFLDPQAPEKLKEAIGGAPDIVLSDMAAPTTGHRQTDHIRT
MHLCEVAAHFAVEVLAEGGHFLAKTFQGGTERDLLNMLKQNFRQVVHVKPASSRAESVEMFLLAKGFKGRHASRSDEPAE
GAAEK
>Mature_244_residues
TKPPVGSNRSGRKLGQKVKKGKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEIDEKHKILAGARRIIDLGAAPG
SWSQIAAKVTNSTDTDPRVAAIDFLEMDAIPGVSFLQMDFLDPQAPEKLKEAIGGAPDIVLSDMAAPTTGHRQTDHIRTM
HLCEVAAHFAVEVLAEGGHFLAKTFQGGTERDLLNMLKQNFRQVVHVKPASSRAESVEMFLLAKGFKGRHASRSDEPAEG
AAEK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=210, Percent_Identity=40.4761904761905, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI194097365, Length=210, Percent_Identity=35.2380952380952, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=35.6020942408377, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=35.6020942408377, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=35.6020942408377, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=42.512077294686, Blast_Score=151, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=39.4871794871795, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553474, Length=206, Percent_Identity=35.9223300970874, Blast_Score=123, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71987550, Length=216, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=216, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=216, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=34.1708542713568, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=34.3589743589744, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=99, Percent_Identity=37.3737373737374, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356387, Length=220, Percent_Identity=40.4545454545455, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=36.3636363636364, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=35.7142857142857, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859957, Length=215, Percent_Identity=31.6279069767442, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 26912; Mature: 26781

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPPVGSNRSGRKLGQKVKKGKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEI
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHH
DEKHKILAGARRIIDLGAAPGSWSQIAAKVTNSTDTDPRVAAIDFLEMDAIPGVSFLQMD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCHHEEEC
FLDPQAPEKLKEAIGGAPDIVLSDMAAPTTGHRQTDHIRTMHLCEVAAHFAVEVLAEGGH
CCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
FLAKTFQGGTERDLLNMLKQNFRQVVHVKPASSRAESVEMFLLAKGFKGRHASRSDEPAE
HHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCC
GAAEK
CCCCC
>Mature Secondary Structure 
TKPPVGSNRSGRKLGQKVKKGKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEI
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHH
DEKHKILAGARRIIDLGAAPGSWSQIAAKVTNSTDTDPRVAAIDFLEMDAIPGVSFLQMD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCHHEEEC
FLDPQAPEKLKEAIGGAPDIVLSDMAAPTTGHRQTDHIRTMHLCEVAAHFAVEVLAEGGH
CCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
FLAKTFQGGTERDLLNMLKQNFRQVVHVKPASSRAESVEMFLLAKGFKGRHASRSDEPAE
HHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCC
GAAEK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]