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The map label for this gene is potF [H]

Identifier: 150395537

GI number: 150395537

Start: 334002

End: 335096

Strand: Direct

Name: potF [H]

Synonym: Smed_0310

Alternate gene names: 150395537

Gene position: 334002-335096 (Clockwise)

Preceding gene: 150395536

Following gene: 150395538

Centisome position: 8.83

GC content: 56.89

Gene sequence:

>1095_bases
ATGTCCAAACTCATCGTCGCAACCCTGACTGCCGCGGTGCTTGCGGGATCGACCATGCTTGCCTCCGCGCAGGAGCGGGT
TGTCAACGTTTACAACTGGTCGGACTATATCGACGACAGCATCCTTGCGGATTTCACCAAGGAGACTGGTATCAAGGTCG
TCTATGACGTCTTCGACTCCAACGAGATTCTGGAAACCAAGCTGCTCGCCGGCGGTTCCGGCTACGACGTCGTCGTCCCG
ACCGCTTATTTCCTACAGCGCCAGATTGCCGCCGGGGTGTTCCAAAAGCTCGACAAGTCGAAACTGCCGAACCTCTCCAA
CATGTGGGACATGGTCATGGAACGTACCGCCCAGTACGATCCGGGCAACGAGTATGCCGTCGACTACATGTGGGGGACGA
CTGGGATCGGCTATAATGTCGACAAGATGAAGGAAATTCTCGGCACCGACGAGAAGCCGAACTGGGATGTCATTTTCGAC
CCGGAGATATCGGCGAAGTTCAAGGACTGCGGCATTCATCTGCTCGACTCGCCGACGGACATCATGCCCTCGGCGCTGGC
CTATCTGGGGCTCAATCCCGACAGCCATGAACAGGCGGATCTCGAAAAGGCAGCCGACCTGCTGATGAAGGTCCGGCCGA
ATATCCGCAAGTTCCATTCCTCGGAATATATCAACGCGCTTGCCAATGGTGACATCTGCCTCGCCGTCGGCTTCTCCGGC
GACATCTTTCAGGCGCGCGACAGAGCGGCCGAGGCGAATGCCGGTGTGACGGTCGACTACTCGATACCGGAACAGGGTGC
CCAGATGTGGTTCGACATGCTGGCGATCCCCGCTGATGCGCCGCATGTCGCCGAAGCGCATGAATTCATCAACTACATGA
TGAAGCCTGAAGTCATCGCCAAGGCGTCGAACTACGTGTTCTACGCCAACGGCAACAAGGCTTCCCAGCAATTCCTCGAC
AAGGAAGTGCTCGAAGACACTGCGATCTATCCGTCCGATGCCGTAATGGAGAAACTCTTCACCACGACGCCTTTCGAGGC
CAAGGAACAGAGGGTTTTGACCCGGCTCTGGACCAGGATCGTCACTGGTCAATAA

Upstream 100 bases:

>100_bases
CGAGAAAGATGCCTAACCTCGACACCGGCGCGATCACAGGCGGGGCATAGCGGCACCGGAAGGATTGCTGTCCAGAGCCG
ATAAATAAGAGGGGAGTTGT

Downstream 100 bases:

>100_bases
AGAGCGGAGACGAATTGCCCGGACCGTAAGATCCGGGCAATTTTCTTGGAGCAGGATGGTACAGACGAGAAGCGTCATCC
TGCCCGCTTCCGCTCCGACT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSKLIVATLTAAVLAGSTMLASAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDVFDSNEILETKLLAGGSGYDVVVP
TAYFLQRQIAAGVFQKLDKSKLPNLSNMWDMVMERTAQYDPGNEYAVDYMWGTTGIGYNVDKMKEILGTDEKPNWDVIFD
PEISAKFKDCGIHLLDSPTDIMPSALAYLGLNPDSHEQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFSG
DIFQARDRAAEANAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVIAKASNYVFYANGNKASQQFLD
KEVLEDTAIYPSDAVMEKLFTTTPFEAKEQRVLTRLWTRIVTGQ

Sequences:

>Translated_364_residues
MSKLIVATLTAAVLAGSTMLASAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDVFDSNEILETKLLAGGSGYDVVVP
TAYFLQRQIAAGVFQKLDKSKLPNLSNMWDMVMERTAQYDPGNEYAVDYMWGTTGIGYNVDKMKEILGTDEKPNWDVIFD
PEISAKFKDCGIHLLDSPTDIMPSALAYLGLNPDSHEQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFSG
DIFQARDRAAEANAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVIAKASNYVFYANGNKASQQFLD
KEVLEDTAIYPSDAVMEKLFTTTPFEAKEQRVLTRLWTRIVTGQ
>Mature_363_residues
SKLIVATLTAAVLAGSTMLASAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDVFDSNEILETKLLAGGSGYDVVVPT
AYFLQRQIAAGVFQKLDKSKLPNLSNMWDMVMERTAQYDPGNEYAVDYMWGTTGIGYNVDKMKEILGTDEKPNWDVIFDP
EISAKFKDCGIHLLDSPTDIMPSALAYLGLNPDSHEQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFSGD
IFQARDRAAEANAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVIAKASNYVFYANGNKASQQFLDK
EVLEDTAIYPSDAVMEKLFTTTPFEAKEQRVLTRLWTRIVTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=50.5494505494505, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787367, Length=302, Percent_Identity=33.7748344370861, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40487; Mature: 40356

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIVATLTAAVLAGSTMLASAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDVFDS
CCCHHHHHHHHHHHHCHHHHHHHHHHHEEHCCCHHHHCHHHHHHHHHHCCCEEEEEECCC
NEILETKLLAGGSGYDVVVPTAYFLQRQIAAGVFQKLDKSKLPNLSNMWDMVMERTAQYD
CHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
PGNEYAVDYMWGTTGIGYNVDKMKEILGTDEKPNWDVIFDPEISAKFKDCGIHLLDSPTD
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCHHHCCEEEECCCHH
IMPSALAYLGLNPDSHEQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCC
DIFQARDRAAEANAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVIA
CHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHE
KASNYVFYANGNKASQQFLDKEVLEDTAIYPSDAVMEKLFTTTPFEAKEQRVLTRLWTRI
ECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VTGQ
HCCC
>Mature Secondary Structure 
SKLIVATLTAAVLAGSTMLASAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDVFDS
CCHHHHHHHHHHHHCHHHHHHHHHHHEEHCCCHHHHCHHHHHHHHHHCCCEEEEEECCC
NEILETKLLAGGSGYDVVVPTAYFLQRQIAAGVFQKLDKSKLPNLSNMWDMVMERTAQYD
CHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
PGNEYAVDYMWGTTGIGYNVDKMKEILGTDEKPNWDVIFDPEISAKFKDCGIHLLDSPTD
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCHHHCCEEEECCCHH
IMPSALAYLGLNPDSHEQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCC
DIFQARDRAAEANAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVIA
CHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHE
KASNYVFYANGNKASQQFLDKEVLEDTAIYPSDAVMEKLFTTTPFEAKEQRVLTRLWTRI
ECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VTGQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]