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The map label for this gene is aglF [H]

Identifier: 150395520

GI number: 150395520

Start: 311839

End: 312921

Strand: Direct

Name: aglF [H]

Synonym: Smed_0293

Alternate gene names: 150395520

Gene position: 311839-312921 (Clockwise)

Preceding gene: 150395519

Following gene: 150395521

Centisome position: 8.25

GC content: 58.82

Gene sequence:

>1083_bases
ATGAAACGGCAGCGGCCGCTATACAAAGCGCCTGGAGCAGATTCCAGAGGCGTGAGAGGGGAGGAATGTGTCATGGAGCA
GTTGATTGCTGCCATTCTCACGATGGTGGCCGGCGTCCTGGTCTGCGCAGCCTATTTCTGGAGCTCGAATCTTCTTCTCG
ACTGGATATTTCCTTCCAAAGGGAAATTCGGTGCCGCCGCCTCGCGCAACCTGCGCTTCGCGAACATGGTACGCCCCTGG
CTGTTTCTCGCCCCGGCTCTGCTGGCGCTTGCGATCTATCTCGTCTATCCCGTCATCAAATCGGTCTTGCTAAGCTTCAA
CGATCGTGGAGGCCAGGATTTCGTGGGCCTGCGCAACTACCTGTGGATGATCAATGACGGCGAGTTCCGTCAGTCGATTT
TCAACAATTTCCTCTGGCTCCTCGTCGTGCCGGCGCTTTCGACTTTCTTCGGCCTGATCATTGCGGCGCTGACCGACCGC
ATCTGGTGGGGCAATATCGCCAAGACGCTGATCTTCATGCCGATGGCGATTTCCTTCGTCGGCGCGGCCGTCATCTGGAA
ATTCATCTATGACTACCGCGCGGCAGGCTCCGACCAGATCGGCCTTTTAAATGCGCTCGTCGTCGCGCTCGGCGGCGAGC
CCCAGGCCTGGATCACGCTCCCCTTCTGGAACAACTTCTTTTTGATGGTCATCCTCATCTGGATTCAGACCGGCTTTGCC
ATGGTCATTCTTTCGGCGGCGCTGCGCGGCATCCCCGAGGAGACGATCGAGGCGGCGGTCATCGACGGCGCCAATGGCTG
GCAGATTTTCTTCAAGATCATGGTGCCGCAGATCTGGGGCACGATCGCCGTCGTCTGGACCACGATCACCATACTGGTCC
TCAAGGTCTTCGACATCGTACTCGCGATGACGAACGGCCAATGGCAGAGCCAGGTGCTCGCCAATCTCATGTTCGACTGG
ATGTTCCGCGGCGGCGGCGACTTCGGTCGCGGCGCAGCCATCGCAGTGGTCATCATGATCCTGGTCGTTCCGATCATGAT
CTGGAACATCCGCAACGCGACCCGGGAAACGGGGGGGCACTGA

Upstream 100 bases:

>100_bases
GCCTGGGACTCGATCAAGTAAAGTCAGGCAAGGCGGAGGGTGGGGGCACCGTCCGCCTTTTTCCTCGATTTCGGGCGGCT
CTCCGGCAAGAGGGCCGCCG

Downstream 100 bases:

>100_bases
GATGAATCCGTCTAGGCGTTCTCCGCTCACCTGGGCGGTGCATCTCTCCGTCCTGCTTCTGGTCCTGCTCTGGACTCTGC
CGACCGCCGGTCTGCTGATT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKRQRPLYKAPGADSRGVRGEECVMEQLIAAILTMVAGVLVCAAYFWSSNLLLDWIFPSKGKFGAAASRNLRFANMVRPW
LFLAPALLALAIYLVYPVIKSVLLSFNDRGGQDFVGLRNYLWMINDGEFRQSIFNNFLWLLVVPALSTFFGLIIAALTDR
IWWGNIAKTLIFMPMAISFVGAAVIWKFIYDYRAAGSDQIGLLNALVVALGGEPQAWITLPFWNNFFLMVILIWIQTGFA
MVILSAALRGIPEETIEAAVIDGANGWQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIVLAMTNGQWQSQVLANLMFDW
MFRGGGDFGRGAAIAVVIMILVVPIMIWNIRNATRETGGH

Sequences:

>Translated_360_residues
MKRQRPLYKAPGADSRGVRGEECVMEQLIAAILTMVAGVLVCAAYFWSSNLLLDWIFPSKGKFGAAASRNLRFANMVRPW
LFLAPALLALAIYLVYPVIKSVLLSFNDRGGQDFVGLRNYLWMINDGEFRQSIFNNFLWLLVVPALSTFFGLIIAALTDR
IWWGNIAKTLIFMPMAISFVGAAVIWKFIYDYRAAGSDQIGLLNALVVALGGEPQAWITLPFWNNFFLMVILIWIQTGFA
MVILSAALRGIPEETIEAAVIDGANGWQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIVLAMTNGQWQSQVLANLMFDW
MFRGGGDFGRGAAIAVVIMILVVPIMIWNIRNATRETGGH
>Mature_360_residues
MKRQRPLYKAPGADSRGVRGEECVMEQLIAAILTMVAGVLVCAAYFWSSNLLLDWIFPSKGKFGAAASRNLRFANMVRPW
LFLAPALLALAIYLVYPVIKSVLLSFNDRGGQDFVGLRNYLWMINDGEFRQSIFNNFLWLLVVPALSTFFGLIIAALTDR
IWWGNIAKTLIFMPMAISFVGAAVIWKFIYDYRAAGSDQIGLLNALVVALGGEPQAWITLPFWNNFFLMVILIWIQTGFA
MVILSAALRGIPEETIEAAVIDGANGWQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIVLAMTNGQWQSQVLANLMFDW
MFRGGGDFGRGAAIAVVIMILVVPIMIWNIRNATRETGGH

Specific function: Part of the binding-protein-dependent transport system for alpha-glucosides such as sucrose, maltose and trehalose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=259, Percent_Identity=25.0965250965251, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787570, Length=259, Percent_Identity=26.2548262548263, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789861, Length=289, Percent_Identity=26.643598615917, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 40198; Mature: 40198

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQRPLYKAPGADSRGVRGEECVMEQLIAAILTMVAGVLVCAAYFWSSNLLLDWIFPSK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
GKFGAAASRNLRFANMVRPWLFLAPALLALAIYLVYPVIKSVLLSFNDRGGQDFVGLRNY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LWMINDGEFRQSIFNNFLWLLVVPALSTFFGLIIAALTDRIWWGNIAKTLIFMPMAISFV
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAVIWKFIYDYRAAGSDQIGLLNALVVALGGEPQAWITLPFWNNFFLMVILIWIQTGFA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
MVILSAALRGIPEETIEAAVIDGANGWQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIV
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAMTNGQWQSQVLANLMFDWMFRGGGDFGRGAAIAVVIMILVVPIMIWNIRNATRETGGH
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRQRPLYKAPGADSRGVRGEECVMEQLIAAILTMVAGVLVCAAYFWSSNLLLDWIFPSK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
GKFGAAASRNLRFANMVRPWLFLAPALLALAIYLVYPVIKSVLLSFNDRGGQDFVGLRNY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LWMINDGEFRQSIFNNFLWLLVVPALSTFFGLIIAALTDRIWWGNIAKTLIFMPMAISFV
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAVIWKFIYDYRAAGSDQIGLLNALVVALGGEPQAWITLPFWNNFFLMVILIWIQTGFA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
MVILSAALRGIPEETIEAAVIDGANGWQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIV
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAMTNGQWQSQVLANLMFDWMFRGGGDFGRGAAIAVVIMILVVPIMIWNIRNATRETGGH
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]