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The map label for this gene is hyi [C]

Identifier: 150395416

GI number: 150395416

Start: 208298

End: 209179

Strand: Direct

Name: hyi [C]

Synonym: Smed_0189

Alternate gene names: 150395416

Gene position: 208298-209179 (Clockwise)

Preceding gene: 150395415

Following gene: 150395417

Centisome position: 5.51

GC content: 65.31

Gene sequence:

>882_bases
ATGAACCCCGTCGGGTTGATCTCCATGCAGTTTGCGCGGCCCTTCACGGCCGAACATTTCCCCCATTTCGCCGGGATGCG
AAGGCTCGGCTACGATTTCGTAGAGCTTCTGGTGCCCGAACCCGGCGAAATCGACCTTGCCGAAACGCGCCGCGCGCTGG
AAGCCGCCGGGCTCTTCGTGGTGCTCGCCGCCCGCGTCAGCCTCCAGCGCAATCTTTCCTCGGACGATCCTGCCGCCCAT
CGCGCCGGCATCGACTATCTGAAATACGCAGCCGACTGCGCCGCCGCGCTCGGCGCCGTGATCGTCGGCGGTCCGCTTAC
CGGAAATCCTCTGGTCTTTGCGGGCCGGCCGCCGCAGCCTGTGAGCGAGGAGGAGCGCCTTTCCCGCAAGGCCCGGTGCG
TCGCCGGTCTGAAAGAGGCCGGTGAGCATGCGGCCGGGCTCGGCGTCATGCTGGCGGTCGAACCGCTGAACCGCTTTGAG
AGCGACGTGCTCTGCACCACCCGGCAGGCGATCGAATTGCTCGATGCGGTTGATCATCCCGCCGTGCAACTGATGCTCGA
CACGTTTCACATGCATATGGAGGAGGCCTCGATTGCCGAAGCAATCCGGCTCGCTGGCAGGCGCATCGTTCATTTCCAGG
CCAACGAGAACCATCGCGGCTTCCCGGGAACGGGCGCCACGGACTGGGTCGCGGTGTTCCGGGCGCTGCACGAGACAGGC
TATCAAGGCCCCATCTCGCTCGAGCCGTTCCGTCGCAACGACGACCGTTTCGGCGTGCCCTTTGCCCAGTGGCGCCCGCC
GCACGAGGACGAGAGCGAACGCCTCGCCGCCAGCGCCGAATTCATCAAGTCCCACATCCTGCTTACGGAATATCGTCGAT
GA

Upstream 100 bases:

>100_bases
TGCCTGCCACCATCCTTTTCCTTCTCGTGCAACGCCGGCTGATCGGCGGTCTGACCCAGGGCGCGGTGAAGGGCTGATTG
CGAATGAAAGAGGACTGACC

Downstream 100 bases:

>100_bases
CACTCAAGATCGGTTGGATCGGCTGTGGCGTGCACGCCAGCCAGATGCTGCTTCCACAGCTCGTTCGCCATGACATCCAG
GTGACTGCGCTCTGCGACAT

Product: xylose isomerase domain-containing protein

Products: 2-Hydroxy-3-oxopropanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNPVGLISMQFARPFTAEHFPHFAGMRRLGYDFVELLVPEPGEIDLAETRRALEAAGLFVVLAARVSLQRNLSSDDPAAH
RAGIDYLKYAADCAAALGAVIVGGPLTGNPLVFAGRPPQPVSEEERLSRKARCVAGLKEAGEHAAGLGVMLAVEPLNRFE
SDVLCTTRQAIELLDAVDHPAVQLMLDTFHMHMEEASIAEAIRLAGRRIVHFQANENHRGFPGTGATDWVAVFRALHETG
YQGPISLEPFRRNDDRFGVPFAQWRPPHEDESERLAASAEFIKSHILLTEYRR

Sequences:

>Translated_293_residues
MNPVGLISMQFARPFTAEHFPHFAGMRRLGYDFVELLVPEPGEIDLAETRRALEAAGLFVVLAARVSLQRNLSSDDPAAH
RAGIDYLKYAADCAAALGAVIVGGPLTGNPLVFAGRPPQPVSEEERLSRKARCVAGLKEAGEHAAGLGVMLAVEPLNRFE
SDVLCTTRQAIELLDAVDHPAVQLMLDTFHMHMEEASIAEAIRLAGRRIVHFQANENHRGFPGTGATDWVAVFRALHETG
YQGPISLEPFRRNDDRFGVPFAQWRPPHEDESERLAASAEFIKSHILLTEYRR
>Mature_293_residues
MNPVGLISMQFARPFTAEHFPHFAGMRRLGYDFVELLVPEPGEIDLAETRRALEAAGLFVVLAARVSLQRNLSSDDPAAH
RAGIDYLKYAADCAAALGAVIVGGPLTGNPLVFAGRPPQPVSEEERLSRKARCVAGLKEAGEHAAGLGVMLAVEPLNRFE
SDVLCTTRQAIELLDAVDHPAVQLMLDTFHMHMEEASIAEAIRLAGRRIVHFQANENHRGFPGTGATDWVAVFRALHETG
YQGPISLEPFRRNDDRFGVPFAQWRPPHEDESERLAASAEFIKSHILLTEYRR

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Escherichia coli, GI1786718, Length=234, Percent_Identity=27.3504273504274, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081874, Length=145, Percent_Identity=30.3448275862069, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22 [C]

Molecular weight: Translated: 32293; Mature: 32293

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVGLISMQFARPFTAEHFPHFAGMRRLGYDFVELLVPEPGEIDLAETRRALEAAGLFV
CCCCCEEEEHHCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VLAARVSLQRNLSSDDPAAHRAGIDYLKYAADCAAALGAVIVGGPLTGNPLVFAGRPPQP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCC
VSEEERLSRKARCVAGLKEAGEHAAGLGVMLAVEPLNRFESDVLCTTRQAIELLDAVDHP
CCHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCH
AVQLMLDTFHMHMEEASIAEAIRLAGRRIVHFQANENHRGFPGTGATDWVAVFRALHETG
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
YQGPISLEPFRRNDDRFGVPFAQWRPPHEDESERLAASAEFIKSHILLTEYRR
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPVGLISMQFARPFTAEHFPHFAGMRRLGYDFVELLVPEPGEIDLAETRRALEAAGLFV
CCCCCEEEEHHCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VLAARVSLQRNLSSDDPAAHRAGIDYLKYAADCAAALGAVIVGGPLTGNPLVFAGRPPQP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCC
VSEEERLSRKARCVAGLKEAGEHAAGLGVMLAVEPLNRFESDVLCTTRQAIELLDAVDHP
CCHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCH
AVQLMLDTFHMHMEEASIAEAIRLAGRRIVHFQANENHRGFPGTGATDWVAVFRALHETG
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
YQGPISLEPFRRNDDRFGVPFAQWRPPHEDESERLAASAEFIKSHILLTEYRR
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: Hydroxypyruvate [C]

Specific reaction: Hydroxypyruvate <==> 2-Hydroxy-3-oxopropanoate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA