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The map label for this gene is argF [H]

Identifier: 150395356

GI number: 150395356

Start: 142052

End: 142963

Strand: Direct

Name: argF [H]

Synonym: Smed_0128

Alternate gene names: 150395356

Gene position: 142052-142963 (Clockwise)

Preceding gene: 150395355

Following gene: 150395357

Centisome position: 3.76

GC content: 63.27

Gene sequence:

>912_bases
ATGACCAGACACTTCCTCGATCTCTCGGCCATGGCGGCTACCGACCTCAGGACAATCATCGACGATGCCCGTGTCCGCAA
GTCGGCGACCAAGGCGGGGACCGCCGAGAAGCCGCTCGCCGGCAAGATGCTGGCGATGATCTTCGAGAAGCCGTCCACCC
GCACGCGCGTTTCCTTCGACGTCGGCATGCGCCAGCTCGGCGGGGAGACTCTGTTTCTGTCGGGCACCGAAATGCAGCTT
GGGCGGGCCGAAACGATCGGGGACACGGCGAAGGTCCTTTCACGCTACGTCGATGCGATCATGATCCGCACAACCGACCA
CCGCCGGCTACTGGAGATGGCGGAGCATGCGACCGTGCCTGTAATCAACGGACTGACTGACGACACGCATCCGTGCCAGA
TCATGGCCGATATCCTGACCTTCGAGGAGCACAGGGGGCCGGTCAAGGGCAAGACGATCGCCTGGACCGGCGACGGCAAC
AATGTGCTTCACTCGCTGATCGAAGGCTCGGCCCGCTTTGGATACCGTATGAACATGGCCGTGCCGCTCGGCTCCGAACC
GCATGACAAGTTTCTGAACTGGGCGCGCAACAACGGGGGCGAGGTCCTGCTTAGCCACGAGCCGGAGCAGGCGGTCGCCG
GTGCCCACTGCGTCGTCACAGACACCTGGATCTCGATGAACCAGGAGCACCGCGCCCGCGGGCATAACGTCTTCCAGCCC
TATCAGGTCAACGGCGCGTTGATGAAGCATGCTGCGCCGGACGCCGTGTTCATGCATTGCCTGCCGGCGCATCGCGGGGA
GGAGGTAACCGACGAAGTCATCGACGGCCCCCAGTCGGTGGTCTTCGATGAAGCGGAAAACCGCCTTCACGCGCAGAAGT
CGATTCTTGCCTGGTGCATGGGCGCGGTCTGA

Upstream 100 bases:

>100_bases
GCGCGGCTCGAGCGCGCCGCGGAAACGATGGCCGGCAAGAGCGGGCACGCCGCGGCTTAAGCTCCACGACAAACGGATTT
ACGACAGGAACCAAGGCAGA

Downstream 100 bases:

>100_bases
GGCGCAAAGGGCGGGCGAGGCGTTCTCTTGAAATCTGTACCGCGCGACACAATCTTGGGCGGGAAGCGGTCGCTTCCTAT
CATCAAATCGGGCAAGCGGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTRHFLDLSAMAATDLRTIIDDARVRKSATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEMQL
GRAETIGDTAKVLSRYVDAIMIRTTDHRRLLEMAEHATVPVINGLTDDTHPCQIMADILTFEEHRGPVKGKTIAWTGDGN
NVLHSLIEGSARFGYRMNMAVPLGSEPHDKFLNWARNNGGEVLLSHEPEQAVAGAHCVVTDTWISMNQEHRARGHNVFQP
YQVNGALMKHAAPDAVFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKSILAWCMGAV

Sequences:

>Translated_303_residues
MTRHFLDLSAMAATDLRTIIDDARVRKSATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEMQL
GRAETIGDTAKVLSRYVDAIMIRTTDHRRLLEMAEHATVPVINGLTDDTHPCQIMADILTFEEHRGPVKGKTIAWTGDGN
NVLHSLIEGSARFGYRMNMAVPLGSEPHDKFLNWARNNGGEVLLSHEPEQAVAGAHCVVTDTWISMNQEHRARGHNVFQP
YQVNGALMKHAAPDAVFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKSILAWCMGAV
>Mature_302_residues
TRHFLDLSAMAATDLRTIIDDARVRKSATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEMQLG
RAETIGDTAKVLSRYVDAIMIRTTDHRRLLEMAEHATVPVINGLTDDTHPCQIMADILTFEEHRGPVKGKTIAWTGDGNN
VLHSLIEGSARFGYRMNMAVPLGSEPHDKFLNWARNNGGEVLLSHEPEQAVAGAHCVVTDTWISMNQEHRARGHNVFQPY
QVNGALMKHAAPDAVFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKSILAWCMGAV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=255, Percent_Identity=46.2745098039216, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI18105007, Length=271, Percent_Identity=28.4132841328413, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=36.3076923076923, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=34.5454545454545, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=26.537216828479, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=287, Percent_Identity=26.8292682926829, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=312, Percent_Identity=33.974358974359, Blast_Score=188, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=26.1437908496732, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=25.8064516129032, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33405; Mature: 33273

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHFLDLSAMAATDLRTIIDDARVRKSATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHH
VGMRQLGGETLFLSGTEMQLGRAETIGDTAKVLSRYVDAIMIRTTDHRRLLEMAEHATVP
HHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCC
VINGLTDDTHPCQIMADILTFEEHRGPVKGKTIAWTGDGNNVLHSLIEGSARFGYRMNMA
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEE
VPLGSEPHDKFLNWARNNGGEVLLSHEPEQAVAGAHCVVTDTWISMNQEHRARGHNVFQP
ECCCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEEEEHHHHCCHHHHHCCCCCCCC
YQVNGALMKHAAPDAVFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKSILAWCM
EECCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHH
GAV
CCC
>Mature Secondary Structure 
TRHFLDLSAMAATDLRTIIDDARVRKSATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHH
VGMRQLGGETLFLSGTEMQLGRAETIGDTAKVLSRYVDAIMIRTTDHRRLLEMAEHATVP
HHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCC
VINGLTDDTHPCQIMADILTFEEHRGPVKGKTIAWTGDGNNVLHSLIEGSARFGYRMNMA
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEE
VPLGSEPHDKFLNWARNNGGEVLLSHEPEQAVAGAHCVVTDTWISMNQEHRARGHNVFQP
ECCCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEEEEHHHHCCHHHHHCCCCCCCC
YQVNGALMKHAAPDAVFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKSILAWCM
EECCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHH
GAV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]