Click here to switch to the map view.

The map label for this gene is phoR [H]

Identifier: 150395346

GI number: 150395346

Start: 131391

End: 132638

Strand: Direct

Name: phoR [H]

Synonym: Smed_0118

Alternate gene names: 150395346

Gene position: 131391-132638 (Clockwise)

Preceding gene: 150395344

Following gene: 150395347

Centisome position: 3.47

GC content: 63.06

Gene sequence:

>1248_bases
ATGGTGTTCGACCGTGCCAAACTGTTGTGGCGTATTGTGGCGCCAAGGCTGGCGGCGGAGCGCTGGCTTCTCTGCCTGTC
CGTGCTGATTGCCGTTCTCGCCGCAGTTGCCGGGATCCATCCGATGTCCGTCCTGCCTTTCTGGGTCATGCTCGCCGCGG
TCATTCTCAGCCGAGGCGCGCCACCGGTCAGGGCGGCGCCGGCAGATGTCGGCGTCGACGTCGAAACCAAGTTCGATCCG
CTTGCTCCCTTGCTCGATGCACTCGACATGCCGGTGCTGGTCGTGGCAGCCGACGAAACCGTCGTCTTCCAGAACGCGGC
CGCCGAGAAGGCATTCGGGACCATTCCCCAGAACAGCTATCTTTCCGCTCGCGTCCGCTCGCCCGGCATCCTTGACATGG
TTCGCGAGACGATTGCGACGGGCAAGGTCAACCAGATCGAACATGCAGAAAGACTGCCTTCGGAGGCGGTCTATGTCGTT
CGCGTCGCGCCGGTGGACATCGGCTCCGACGTTTCCGGCCGGCGCATCTTCGTGCTGACCTATCGCGATATCTCGCAGGC
CCGCCGCATCGATCGGATGCGTTCCGACTTCGTCGCCAATGCCAGCCATGAGCTGCGCACGCCGCTTGCTTCGCTGCGCG
GCTTCATCGAGACGCTGCAGGGGCCGGCCCGCGAAGACCGGAAGGCACAGGAGAAATTCTTCGGCATCATGCACGAACAG
GTCACCCGCATGAGTCGGCTCGTGGACGACCTCCTGTCACTGTCGAGGCTCGAGTTGAAGTCGCACATCGCCCCCGACGA
CACCGTCGATCTGGGGCCGCTGCTCGGACATGTGCGCGACAGTCTGGCGCCGCTTGCCGGAGAACTGGATGTGACGATCG
CGCTGGAGGTCCCGAATACGCCTGTTTTCGTCCAGGGGGACCGTGACGAACTGGTGGAGGTTTTCGAAAACCTGATCGAG
AATGCCTGCAAATACGGGCAGGAAGGCAAGAAGGTGGAGGTCCGGCTGACCGGTGGAGAAGGAAACCAGGCGGAGGTGAC
GGTCACCGACCACGGCGCGGGAATCCCGGCCGAGCACGTCCCGCGCATTACCGAGCGCTTTTACCGGGTCAACGTCGAGG
CGAGCCGGTCGAAGAAAGGCACCGGATTGGGTCTTGCGATCGTCAAGCATATCCTCACGCGCCACCGGGCTCGCCTGCTC
GTCCATTCGGAAGTGGGCAAGGGTACGGTCTTTACGGTTCGTTTCTGA

Upstream 100 bases:

>100_bases
GGGGCCGATTTCCTCATCGCGCTCCGACACGAATTATGGCCATGGACATGGTCAGTAAAGCCATTCAGATGCAGGAGGCC
GCGAATTGAGGGAAGAGGGT

Downstream 100 bases:

>100_bases
CACATAAGACGGTCCATTGCTGCTGCAGCAGAATTTTATCAAGCATTTTCAGCTCGTTAGACTGTCATAAATGTTTCACC
AAACTGACATAAAAGCTGTA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVFDRAKLLWRIVAPRLAAERWLLCLSVLIAVLAAVAGIHPMSVLPFWVMLAAVILSRGAPPVRAAPADVGVDVETKFDP
LAPLLDALDMPVLVVAADETVVFQNAAAEKAFGTIPQNSYLSARVRSPGILDMVRETIATGKVNQIEHAERLPSEAVYVV
RVAPVDIGSDVSGRRIFVLTYRDISQARRIDRMRSDFVANASHELRTPLASLRGFIETLQGPAREDRKAQEKFFGIMHEQ
VTRMSRLVDDLLSLSRLELKSHIAPDDTVDLGPLLGHVRDSLAPLAGELDVTIALEVPNTPVFVQGDRDELVEVFENLIE
NACKYGQEGKKVEVRLTGGEGNQAEVTVTDHGAGIPAEHVPRITERFYRVNVEASRSKKGTGLGLAIVKHILTRHRARLL
VHSEVGKGTVFTVRF

Sequences:

>Translated_415_residues
MVFDRAKLLWRIVAPRLAAERWLLCLSVLIAVLAAVAGIHPMSVLPFWVMLAAVILSRGAPPVRAAPADVGVDVETKFDP
LAPLLDALDMPVLVVAADETVVFQNAAAEKAFGTIPQNSYLSARVRSPGILDMVRETIATGKVNQIEHAERLPSEAVYVV
RVAPVDIGSDVSGRRIFVLTYRDISQARRIDRMRSDFVANASHELRTPLASLRGFIETLQGPAREDRKAQEKFFGIMHEQ
VTRMSRLVDDLLSLSRLELKSHIAPDDTVDLGPLLGHVRDSLAPLAGELDVTIALEVPNTPVFVQGDRDELVEVFENLIE
NACKYGQEGKKVEVRLTGGEGNQAEVTVTDHGAGIPAEHVPRITERFYRVNVEASRSKKGTGLGLAIVKHILTRHRARLL
VHSEVGKGTVFTVRF
>Mature_415_residues
MVFDRAKLLWRIVAPRLAAERWLLCLSVLIAVLAAVAGIHPMSVLPFWVMLAAVILSRGAPPVRAAPADVGVDVETKFDP
LAPLLDALDMPVLVVAADETVVFQNAAAEKAFGTIPQNSYLSARVRSPGILDMVRETIATGKVNQIEHAERLPSEAVYVV
RVAPVDIGSDVSGRRIFVLTYRDISQARRIDRMRSDFVANASHELRTPLASLRGFIETLQGPAREDRKAQEKFFGIMHEQ
VTRMSRLVDDLLSLSRLELKSHIAPDDTVDLGPLLGHVRDSLAPLAGELDVTIALEVPNTPVFVQGDRDELVEVFENLIE
NACKYGQEGKKVEVRLTGGEGNQAEVTVTDHGAGIPAEHVPRITERFYRVNVEASRSKKGTGLGLAIVKHILTRHRARLL
VHSEVGKGTVFTVRF

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=39.4067796610169, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=33.7606837606838, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=32.1428571428571, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=31.3636363636364, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994928, Length=272, Percent_Identity=28.6764705882353, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788713, Length=378, Percent_Identity=24.0740740740741, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=234, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790346, Length=201, Percent_Identity=29.3532338308458, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789403, Length=199, Percent_Identity=32.1608040201005, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082128, Length=255, Percent_Identity=27.0588235294118, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=224, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787374, Length=235, Percent_Identity=26.3829787234043, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=23.8866396761134, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=199, Percent_Identity=32.6633165829146, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788279, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790551, Length=220, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789808, Length=209, Percent_Identity=27.7511961722488, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=238, Percent_Identity=27.3109243697479, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45548; Mature: 45548

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFDRAKLLWRIVAPRLAAERWLLCLSVLIAVLAAVAGIHPMSVLPFWVMLAAVILSRGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
PPVRAAPADVGVDVETKFDPLAPLLDALDMPVLVVAADETVVFQNAAAEKAFGTIPQNSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHCCCCCCCC
LSARVRSPGILDMVRETIATGKVNQIEHAERLPSEAVYVVRVAPVDIGSDVSGRRIFVLT
EEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEE
YRDISQARRIDRMRSDFVANASHELRTPLASLRGFIETLQGPAREDRKAQEKFFGIMHEQ
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VTRMSRLVDDLLSLSRLELKSHIAPDDTVDLGPLLGHVRDSLAPLAGELDVTIALEVPNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
PVFVQGDRDELVEVFENLIENACKYGQEGKKVEVRLTGGEGNQAEVTVTDHGAGIPAEHV
CEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHH
PRITERFYRVNVEASRSKKGTGLGLAIVKHILTRHRARLLVHSEVGKGTVFTVRF
HHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MVFDRAKLLWRIVAPRLAAERWLLCLSVLIAVLAAVAGIHPMSVLPFWVMLAAVILSRGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
PPVRAAPADVGVDVETKFDPLAPLLDALDMPVLVVAADETVVFQNAAAEKAFGTIPQNSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHCCCCCCCC
LSARVRSPGILDMVRETIATGKVNQIEHAERLPSEAVYVVRVAPVDIGSDVSGRRIFVLT
EEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEE
YRDISQARRIDRMRSDFVANASHELRTPLASLRGFIETLQGPAREDRKAQEKFFGIMHEQ
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VTRMSRLVDDLLSLSRLELKSHIAPDDTVDLGPLLGHVRDSLAPLAGELDVTIALEVPNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
PVFVQGDRDELVEVFENLIENACKYGQEGKKVEVRLTGGEGNQAEVTVTDHGAGIPAEHV
CEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHH
PRITERFYRVNVEASRSKKGTGLGLAIVKHILTRHRARLLVHSEVGKGTVFTVRF
HHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]