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The map label for this gene is gacS [H]

Identifier: 150395255

GI number: 150395255

Start: 22461

End: 24680

Strand: Direct

Name: gacS [H]

Synonym: Smed_0027

Alternate gene names: 150395255

Gene position: 22461-24680 (Clockwise)

Preceding gene: 150395254

Following gene: 150395256

Centisome position: 0.59

GC content: 66.49

Gene sequence:

>2220_bases
ATGAGGGCTGGCGCGGAAACGGCACGGGAGGCCGGCGGGACCGCCGCGCCAGCAGCGCCTACGGTGAAGATCGGCGCCGC
GCTCCTTTGGCAGCGCCGGTTTTGGGCTGATCCTTCGGCAGGGGCGCCGGTCGCGCGGCTCGCCTATGCTCTCGCCGGCA
TCGCTGCGGCAATGCTGCTCCTCGCGGTCGGTGCGAGCGCGGGTCTGCACCTGTTGTCTGCGATCATTGCGGCCGGGGGC
ATCGCCGGTGCCTTCCTGCTGCTGTCCGGTCGAGACGAAGCGTCCGGGAGCCTTAGGGCCGGCGCCCGCGAAACTGCGCA
GGACGCCGCGGCGCCCCTTGCATCCATCCATGACGCCATGGGCGATCTCGTCATCGTCCGCGACCCCGACGGCAAGGTCA
TGCAGGCCAATGTCGCCTTCCATGAGCTTTGCGGATGCGCCGACGCGCGGGGCCTGACCTGCGCGGAGCTGGGATTGCGC
TTCGAGCCGAAGCCGGACCCCTACCGCTACTTCGTGCATATTTGTACGTCCGCAGGCACGCGCCTCTACGATTGGCACGA
CGTGCTTGTCCGCGACGCGCATGGCCGGCTGATGCGCCATAGCATTGCTCGAGACGTCACGGAGGATATGCTGGCGGCAA
GCCAGAGAGAGGAGGCGAGGCAGCGCGCCGAGGAGGCGAGCCGGGCCAAGTCCCGTCTGCTCGCCACCGTCAGTCATGAG
ATCCGTACGCCGCTTTCCGGCATCCTCGGTATGAGCCGGCTCCTCGCAGAGACGCGGCTGTCGGACGAGCAGAAGAACTA
TCTCGTCGGCATGCAGCAGTCCGGACACACCCTGGTCCAACTCGTCGAGGACCTGATCGATTTTTCCTCGCTTGCCGCCG
GACGGTTCCAGCTGCGCCCGTCGCAGCAGGACCTGCGTCAGACGGTCGAGAACGTGGTCGAGATGCTGTCTCCCCGCGCG
CATGAGAAGGGTATCGAGATAGGTGCAACGGTCGCCATCGAGATACCCGAGCGGATGCTGTTCGATGCGGCGCGCCTGCG
CCAGGTGCTGTTCAACGTGGTCGGCAACGCGGTGAAATTCACCGAGAAGGGCGGCGTTATCGTCTCTGTCGATATCGAGA
ATGGCTCGTTGCGCATCCGGGTTGACGATAGCGGACCCGGCATGTCCGCCGACGCGCTCGCACGCGTCTTCGAGGAGTTC
GAGCAGGCCGGTGACGAGGCCCAACGTGCGAAGGGAACGGGGCTCGGCCTCGCAATATCCCGGCGGATCATGGAAGCTTT
CGGCGGCAGCCTTACCGCCACGAGCGTTTCCGGCAAGGGAAGCCGTTTCGAGATCCGGTTCCCGATGGATGTCGCCGGTC
TCTCGACAGTTCCGGTTCGCCGCGGCATTCTGGCGGGTGCGCATGTGCTTGTCATGGCGCCCGAAGGGCCTTCCTCAGCC
GCGCTTGCCGCCACGATCGAGACGCTGGGCGCCACGTGCCATAGGGCTTCGACGCTTGCCGTCGCGCGGCGGATCGTCGC
CGGCGCGCTCGCCAATCGGCTGCTGCTGACGGATATCATTGTCGATCACCGCCACGCTGGACAGTCTCGCGAGCTCCTTG
CTCTCGAGCCGGCGATCGCCGGCCTGGGTTTGCGCAGAACCTATCTCATCAGCCCCGAGGAGCGGGCGGGCCATCCCGTG
AGCCGACTCGGGGGCTATGAGGCCTGGCTGATCCGCCCTCTTCGCGAGAAATCGCTGATTGAGGTGCTACTTGGGCGACT
CAGGGGAATGGAAAAGCGCGATGCGATCAACGACAACAGACCGGTGCTGCGGGAGGAGCCAGCGGCCGTGAAGGCGGATG
TTCGCGCCATCCTGCTTGCAGAGGACGACCCTGTGAACGCGCTCGTCTTGCAGTCGGTTCTCAGCCGGGCCGGCCATGCC
GTCGATCACGTCGGCGACTTCATGGCGCTCGAGGCCGCAATCCGCTCGGCCGCGCCGCCCCTGATCGTCACAGACCTCAA
CATGCCAGGGGGCGATGGTCTCGACATGCTCCGACGTCTTCGCGAAGCGGAGGCCGCTGGCAACAGGCGGCGTGTGCCGG
TTCTCGTCCTGACCGCGGACGCGCGCGCCGATATCTATGAGCGCCTTCGAGTTGCGGGAGCGGACGCCGTGCTGGTGAAG
CCTGCCGATCCGCAGCGGCTTCTTGCCGAAGTCACCCGCCTCATCCACCCCGCAGCATAG

Upstream 100 bases:

>100_bases
AAAAGGAACGGTCGCTAAAACTTTAGACGTCGGCTGAAGGCATTCGGAAGGCTCGCCATGGTAGTCCGCGGGCATGAGCG
AACGGATGTTTCGAGGGCGT

Downstream 100 bases:

>100_bases
ATTTCCACGCTTACGGCCCCTTACCAGGTCGGGGTTGTATACTGCCCCGGTGTCACTATGTTGTTGCAGAAACGTGGTCT
AAGCCCGACAGGAATGACGG

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MRAGAETAREAGGTAAPAAPTVKIGAALLWQRRFWADPSAGAPVARLAYALAGIAAAMLLLAVGASAGLHLLSAIIAAGG
IAGAFLLLSGRDEASGSLRAGARETAQDAAAPLASIHDAMGDLVIVRDPDGKVMQANVAFHELCGCADARGLTCAELGLR
FEPKPDPYRYFVHICTSAGTRLYDWHDVLVRDAHGRLMRHSIARDVTEDMLAASQREEARQRAEEASRAKSRLLATVSHE
IRTPLSGILGMSRLLAETRLSDEQKNYLVGMQQSGHTLVQLVEDLIDFSSLAAGRFQLRPSQQDLRQTVENVVEMLSPRA
HEKGIEIGATVAIEIPERMLFDAARLRQVLFNVVGNAVKFTEKGGVIVSVDIENGSLRIRVDDSGPGMSADALARVFEEF
EQAGDEAQRAKGTGLGLAISRRIMEAFGGSLTATSVSGKGSRFEIRFPMDVAGLSTVPVRRGILAGAHVLVMAPEGPSSA
ALAATIETLGATCHRASTLAVARRIVAGALANRLLLTDIIVDHRHAGQSRELLALEPAIAGLGLRRTYLISPEERAGHPV
SRLGGYEAWLIRPLREKSLIEVLLGRLRGMEKRDAINDNRPVLREEPAAVKADVRAILLAEDDPVNALVLQSVLSRAGHA
VDHVGDFMALEAAIRSAAPPLIVTDLNMPGGDGLDMLRRLREAEAAGNRRRVPVLVLTADARADIYERLRVAGADAVLVK
PADPQRLLAEVTRLIHPAA

Sequences:

>Translated_739_residues
MRAGAETAREAGGTAAPAAPTVKIGAALLWQRRFWADPSAGAPVARLAYALAGIAAAMLLLAVGASAGLHLLSAIIAAGG
IAGAFLLLSGRDEASGSLRAGARETAQDAAAPLASIHDAMGDLVIVRDPDGKVMQANVAFHELCGCADARGLTCAELGLR
FEPKPDPYRYFVHICTSAGTRLYDWHDVLVRDAHGRLMRHSIARDVTEDMLAASQREEARQRAEEASRAKSRLLATVSHE
IRTPLSGILGMSRLLAETRLSDEQKNYLVGMQQSGHTLVQLVEDLIDFSSLAAGRFQLRPSQQDLRQTVENVVEMLSPRA
HEKGIEIGATVAIEIPERMLFDAARLRQVLFNVVGNAVKFTEKGGVIVSVDIENGSLRIRVDDSGPGMSADALARVFEEF
EQAGDEAQRAKGTGLGLAISRRIMEAFGGSLTATSVSGKGSRFEIRFPMDVAGLSTVPVRRGILAGAHVLVMAPEGPSSA
ALAATIETLGATCHRASTLAVARRIVAGALANRLLLTDIIVDHRHAGQSRELLALEPAIAGLGLRRTYLISPEERAGHPV
SRLGGYEAWLIRPLREKSLIEVLLGRLRGMEKRDAINDNRPVLREEPAAVKADVRAILLAEDDPVNALVLQSVLSRAGHA
VDHVGDFMALEAAIRSAAPPLIVTDLNMPGGDGLDMLRRLREAEAAGNRRRVPVLVLTADARADIYERLRVAGADAVLVK
PADPQRLLAEVTRLIHPAA
>Mature_739_residues
MRAGAETAREAGGTAAPAAPTVKIGAALLWQRRFWADPSAGAPVARLAYALAGIAAAMLLLAVGASAGLHLLSAIIAAGG
IAGAFLLLSGRDEASGSLRAGARETAQDAAAPLASIHDAMGDLVIVRDPDGKVMQANVAFHELCGCADARGLTCAELGLR
FEPKPDPYRYFVHICTSAGTRLYDWHDVLVRDAHGRLMRHSIARDVTEDMLAASQREEARQRAEEASRAKSRLLATVSHE
IRTPLSGILGMSRLLAETRLSDEQKNYLVGMQQSGHTLVQLVEDLIDFSSLAAGRFQLRPSQQDLRQTVENVVEMLSPRA
HEKGIEIGATVAIEIPERMLFDAARLRQVLFNVVGNAVKFTEKGGVIVSVDIENGSLRIRVDDSGPGMSADALARVFEEF
EQAGDEAQRAKGTGLGLAISRRIMEAFGGSLTATSVSGKGSRFEIRFPMDVAGLSTVPVRRGILAGAHVLVMAPEGPSSA
ALAATIETLGATCHRASTLAVARRIVAGALANRLLLTDIIVDHRHAGQSRELLALEPAIAGLGLRRTYLISPEERAGHPV
SRLGGYEAWLIRPLREKSLIEVLLGRLRGMEKRDAINDNRPVLREEPAAVKADVRAILLAEDDPVNALVLQSVLSRAGHA
VDHVGDFMALEAAIRSAAPPLIVTDLNMPGGDGLDMLRRLREAEAAGNRRRVPVLVLTADARADIYERLRVAGADAVLVK
PADPQRLLAEVTRLIHPAA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=562, Percent_Identity=29.1814946619217, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI145693157, Length=563, Percent_Identity=27.886323268206, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI48994928, Length=381, Percent_Identity=31.496062992126, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=37.0967741935484, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788713, Length=278, Percent_Identity=30.5755395683453, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=27.4193548387097, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=30.0813008130081, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788549, Length=272, Percent_Identity=25.7352941176471, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=25.8474576271186, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788279, Length=214, Percent_Identity=25.2336448598131, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786600, Length=254, Percent_Identity=27.9527559055118, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=29.3859649122807, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=204, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787894, Length=222, Percent_Identity=25.2252252252252, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1790861, Length=217, Percent_Identity=26.2672811059908, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 78887; Mature: 78887

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGAETAREAGGTAAPAAPTVKIGAALLWQRRFWADPSAGAPVARLAYALAGIAAAMLL
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LAVGASAGLHLLSAIIAAGGIAGAFLLLSGRDEASGSLRAGARETAQDAAAPLASIHDAM
HHHCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GDLVIVRDPDGKVMQANVAFHELCGCADARGLTCAELGLRFEPKPDPYRYFVHICTSAGT
CCEEEEECCCCCEEEECHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCC
RLYDWHDVLVRDAHGRLMRHSIARDVTEDMLAASQREEARQRAEEASRAKSRLLATVSHE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTPLSGILGMSRLLAETRLSDEQKNYLVGMQQSGHTLVQLVEDLIDFSSLAAGRFQLRP
HHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECC
SQQDLRQTVENVVEMLSPRAHEKGIEIGATVAIEIPERMLFDAARLRQVLFNVVGNAVKF
CHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
TEKGGVIVSVDIENGSLRIRVDDSGPGMSADALARVFEEFEQAGDEAQRAKGTGLGLAIS
ECCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHH
RRIMEAFGGSLTATSVSGKGSRFEIRFPMDVAGLSTVPVRRGILAGAHVLVMAPEGPSSA
HHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCH
ALAATIETLGATCHRASTLAVARRIVAGALANRLLLTDIIVDHRHAGQSRELLALEPAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHH
GLGLRRTYLISPEERAGHPVSRLGGYEAWLIRPLREKSLIEVLLGRLRGMEKRDAINDNR
CCCCCEEEEECCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
PVLREEPAAVKADVRAILLAEDDPVNALVLQSVLSRAGHAVDHVGDFMALEAAIRSAAPP
CCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
LIVTDLNMPGGDGLDMLRRLREAEAAGNRRRVPVLVLTADARADIYERLRVAGADAVLVK
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEC
PADPQRLLAEVTRLIHPAA
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRAGAETAREAGGTAAPAAPTVKIGAALLWQRRFWADPSAGAPVARLAYALAGIAAAMLL
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LAVGASAGLHLLSAIIAAGGIAGAFLLLSGRDEASGSLRAGARETAQDAAAPLASIHDAM
HHHCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GDLVIVRDPDGKVMQANVAFHELCGCADARGLTCAELGLRFEPKPDPYRYFVHICTSAGT
CCEEEEECCCCCEEEECHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCC
RLYDWHDVLVRDAHGRLMRHSIARDVTEDMLAASQREEARQRAEEASRAKSRLLATVSHE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTPLSGILGMSRLLAETRLSDEQKNYLVGMQQSGHTLVQLVEDLIDFSSLAAGRFQLRP
HHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECC
SQQDLRQTVENVVEMLSPRAHEKGIEIGATVAIEIPERMLFDAARLRQVLFNVVGNAVKF
CHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
TEKGGVIVSVDIENGSLRIRVDDSGPGMSADALARVFEEFEQAGDEAQRAKGTGLGLAIS
ECCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHH
RRIMEAFGGSLTATSVSGKGSRFEIRFPMDVAGLSTVPVRRGILAGAHVLVMAPEGPSSA
HHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCH
ALAATIETLGATCHRASTLAVARRIVAGALANRLLLTDIIVDHRHAGQSRELLALEPAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHH
GLGLRRTYLISPEERAGHPVSRLGGYEAWLIRPLREKSLIEVLLGRLRGMEKRDAINDNR
CCCCCEEEEECCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
PVLREEPAAVKADVRAILLAEDDPVNALVLQSVLSRAGHAVDHVGDFMALEAAIRSAAPP
CCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
LIVTDLNMPGGDGLDMLRRLREAEAAGNRRRVPVLVLTADARADIYERLRVAGADAVLVK
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEC
PADPQRLLAEVTRLIHPAA
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]