Click here to switch to the map view.

The map label for this gene is ctfb

Identifier: 15004867

GI number: 15004867

Start: 179117

End: 179782

Strand: Direct

Name: ctfb

Synonym: CA_P0164

Alternate gene names: 15004867

Gene position: 179117-179782 (Clockwise)

Preceding gene: 15004866

Following gene: 15004869

Centisome position: 93.29

GC content: 35.59

Gene sequence:

>666_bases
ATGATTAATGATAAAAACCTAGCGAAAGAAATAATAGCCAAAAGAGTTGCAAGAGAATTAAAAAATGGTCAACTTGTAAA
CTTAGGTGTAGGTCTTCCTACCATGGTTGCAGATTATATACCAAAAAATTTCAAAATTACTTTCCAATCAGAAAACGGAA
TAGTTGGAATGGGCGCTAGTCCTAAAATAAATGAGGCAGATAAAGATGTAGTAAATGCAGGAGGAGACTATACAACAGTA
CTTCCTGACGGCACATTTTTCGATAGCTCAGTTTCGTTTTCACTAATCCGTGGTGGTCACGTAGATGTTACTGTTTTAGG
GGCTCTCCAGGTAGATGAAAAGGGTAATATAGCCAATTGGATTGTTCCTGGAAAAATGCTCTCTGGTATGGGTGGAGCTA
TGGATTTAGTAAATGGAGCTAAGAAAGTAATAATTGCAATGAGACATACAAATAAAGGTCAACCTAAAATTTTAAAAAAA
TGTACACTTCCCCTCACGGCAAAGTCTCAAGCAAATCTAATTGTAACAGAACTTGGAGTAATTGAGGTTATTAATGATGG
TTTACTTCTCACTGAAATTAATAAAAACACAACCATTGATGAAATAAGGTCTTTAACTGCTGCAGATTTACTCATATCCA
ATGAACTTAGACCCATGGCTGTTTAG

Upstream 100 bases:

>100_bases
GTTGAAGCTGAAAATTTAGTTAGCTGTGAAAAACTAGAAAAGGAAAAAGCAATGACCCCCGGAGTTCTTATAAATTATAT
AGTAAAGGAGCCTGCATAAA

Downstream 100 bases:

>100_bases
AAAGAAATACTATGAAACAATATTAAAAAAATAAGAGTTACCATTTAAGGTAACTCTTATTTTTATTACTTAAGATAATC
ATATATAACTTCAGCTCTAG

Product: butyrate-acetoacetate COA-transferase subunit B

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGASPKINEADKDVVNAGGDYTTV
LPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKK
CTLPLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV

Sequences:

>Translated_221_residues
MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGASPKINEADKDVVNAGGDYTTV
LPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKK
CTLPLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV
>Mature_221_residues
MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGASPKINEADKDVVNAGGDYTTV
LPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKK
CTLPLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family

Homologues:

Organism=Homo sapiens, GI4557817, Length=214, Percent_Identity=48.1308411214953, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=44.1860465116279, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1788552, Length=210, Percent_Identity=53.8095238095238, Blast_Score=235, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=46.9767441860465, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=46.2616822429907, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=46.2616822429907, Blast_Score=201, Evalue=4e-52,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): CTFB_CLOAB (P23673)

Other databases:

- EMBL:   M55392
- EMBL:   X72831
- EMBL:   M93363
- EMBL:   AE001438
- PIR:   JN0489
- RefSeq:   NP_149327.1
- ProteinModelPortal:   P23673
- GeneID:   1116169
- GenomeReviews:   AE001438_GR
- KEGG:   cac:CA_P0164
- NMPDR:   fig|272562.1.peg.162
- HOGENOM:   HBG353770
- OMA:   HYDPTIV
- PhylomeDB:   P23673
- BioCyc:   CACE272562:CAP0164-MONOMER
- BRENDA:   2.8.3.9
- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164
- PANTHER:   PTHR13707
- SMART:   SM00882
- TIGRFAMs:   TIGR02428

Pfam domain/function: PF01144 CoA_trans

EC number: =2.8.3.9

Molecular weight: Translated: 23624; Mature: 23624

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01274 COA_TRANSF_2

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGAS
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEEECCCCEEECCCC
PKINEADKDVVNAGGDYTTVLPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANW
CCCCCCCHHHHHCCCCEEEECCCCCEECCCCEEEEEECCCEEEEEEEEEEECCCCCEEEE
IVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKKCTLPLTAKSQANLIVTELGV
EECHHHHHCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCCEECCCCCCEEEEECCE
IEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGAS
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEEECCCCEEECCCC
PKINEADKDVVNAGGDYTTVLPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANW
CCCCCCCHHHHHCCCCEEEECCCCCEECCCCEEEEEECCCEEEEEEEEEEECCCCCEEEE
IVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKKCTLPLTAKSQANLIVTELGV
EECHHHHHCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCCEECCCCCCEEEEECCE
IEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002