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The map label for this gene is ctfa

Identifier: 15004866

GI number: 15004866

Start: 178459

End: 179115

Strand: Direct

Name: ctfa

Synonym: CA_P0163

Alternate gene names: 15004866

Gene position: 178459-179115 (Clockwise)

Preceding gene: 15004865

Following gene: 15004867

Centisome position: 92.95

GC content: 32.12

Gene sequence:

>657_bases
ATGAACTCTAAAATAATTAGATTTGAAAATTTAAGGTCATTCTTTAAAGATGGGATGACAATTATGATTGGAGGTTTTTT
AAACTGTGGCACTCCAACCAAATTAATTGATTTTTTAGTTAATTTAAATATAAAGAATTTAACGATTATAAGTAATGATA
CATGTTATCCTAATACAGGTATTGGTAAGTTAATATCAAATAATCAAGTAAAAAAGCTTATTGCTTCATATATAGGCAGC
AACCCAGATACTGGCAAAAAACTTTTTAATAATGAACTTGAAGTAGAGCTCTCTCCCCAAGGAACTCTAGTGGAAAGAAT
ACGTGCAGGCGGATCTGGCTTAGGTGGTGTACTAACTAAAACAGGTTTAGGAACTTTGATTGAAAAAGGAAAGAAAAAAA
TATCTATAAATGGAACGGAATATTTGTTAGAGCTACCTCTTACAGCCGATGTAGCATTAATTAAAGGTAGTATTGTAGAT
GAGGCCGGAAACACCTTCTATAAAGGTACTACTAAAAACTTTAATCCCTATATGGCAATGGCAGCTAAAACCGTAATAGT
TGAAGCTGAAAATTTAGTTAGCTGTGAAAAACTAGAAAAGGAAAAAGCAATGACCCCCGGAGTTCTTATAAATTATATAG
TAAAGGAGCCTGCATAA

Upstream 100 bases:

>100_bases
AAAAGAAATGTATATAAATTGTTTTAAAAAACAACCTTAAACCTTCATATTTCAACTACTTTTTATAATTTTAATAAAGA
ATTTAAAAGGAGGGATTAAA

Downstream 100 bases:

>100_bases
AATGATTAATGATAAAAACCTAGCGAAAGAAATAATAGCCAAAAGAGTTGCAAGAGAATTAAAAAATGGTCAACTTGTAA
ACTTAGGTGTAGGTCTTCCT

Product: butyrate-acetoacetate COA-transferase subunit A

Products: NA

Alternate protein names: Coat A; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGS
NPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVD
EAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA

Sequences:

>Translated_218_residues
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGS
NPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVD
EAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA
>Mature_218_residues
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGS
NPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVD
EAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation

COG id: COG1788

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit A family

Homologues:

Organism=Homo sapiens, GI4557817, Length=217, Percent_Identity=37.7880184331797, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI11545841, Length=217, Percent_Identity=36.8663594470046, Blast_Score=144, Evalue=8e-35,
Organism=Escherichia coli, GI1788551, Length=214, Percent_Identity=46.7289719626168, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17531517, Length=218, Percent_Identity=35.3211009174312, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24655794, Length=218, Percent_Identity=37.6146788990826, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358325, Length=218, Percent_Identity=37.6146788990826, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CTFA_CLOAB (P33752)

Other databases:

- EMBL:   X72831
- EMBL:   M93363
- EMBL:   AE001438
- EMBL:   L14817
- PIR:   B49346
- RefSeq:   NP_149326.1
- ProteinModelPortal:   P33752
- GeneID:   1116168
- GenomeReviews:   AE001438_GR
- KEGG:   cac:CA_P0163
- NMPDR:   fig|272562.1.peg.161
- HOGENOM:   HBG288750
- OMA:   YLLELPI
- ProtClustDB:   CLSK865492
- BioCyc:   CACE272562:CAP0163-MONOMER
- BRENDA:   2.8.3.9
- InterPro:   IPR012792
- InterPro:   IPR004165
- InterPro:   IPR004163
- PANTHER:   PTHR13707
- SMART:   SM00882
- TIGRFAMs:   TIGR02429

Pfam domain/function: PF01144 CoA_trans

EC number: =2.8.3.9

Molecular weight: Translated: 23641; Mature: 23641

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS01273 COA_TRANSF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTG
CCCCEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC
IGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTK
CHHHHCCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCCCCHHHHH
TGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYMAM
HHHHHHHHCCCCEEEECCCEEEEECCCCCCHHHHHCCHHHCCCCEEECCCCCCCCHHHHH
AAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA
HHHEEEEECHHHHHHHHHHHHHCCCCCHHHHHEECCCC
>Mature Secondary Structure
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTG
CCCCEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC
IGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTK
CHHHHCCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCCCCHHHHH
TGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYMAM
HHHHHHHHCCCCEEEECCCEEEEECCCCCCHHHHHCCHHHCCCCEEECCCCCCCCHHHHH
AAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA
HHHEEEEECHHHHHHHHHHHHHCCCCCHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226639; 8423010; 11466286; 8300540; 2383002