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The map label for this gene is pdc

Identifier: 15004729

GI number: 15004729

Start: 24954

End: 26618

Strand: Direct

Name: pdc

Synonym: CA_P0025

Alternate gene names: 15004729

Gene position: 24954-26618 (Clockwise)

Preceding gene: 15004719

Following gene: 15004730

Centisome position: 13.0

GC content: 34.47

Gene sequence:

>1665_bases
TTGAAGAGTGAATACACAATTGGAAGATATTTGTTAGACCGTTTATCAGAGTTGGGTATTCGGCATATCTTTGGTGTACC
TGGAGATTACAATCTATCCTTTTTAGACTATATAATGGAGTACAAAGGGATAGATTGGGTTGGAAATTGCAATGAATTGA
ATGCTGGGTATGCTGCTGATGGATATGCAAGAATAAATGGAATTGGAGCCATACTTACAACATTTGGTGTTGGAGAATTA
AGTGCCATTAACGCAATTGCTGGGGCATACGCTGAGCAAGTTCCAGTTGTTAAAATTACAGGTATCCCCACAGCAAAAGT
TAGGGACAATGGATTATATGTACACCACACATTAGGTGACGGAAGGTTTGATCACTTTTTTGAAATGTTTAGAGAAGTAA
CAGTTGCTGAGGCATTACTAAGCGAAGAAAATGCAGCACAAGAAATTGATCGTGTTCTTATTTCATGCTGGAGACAAAAA
CGTCCTGTTCTTATAAATTTACCGATTGATGTATATGATAAACCAATTAACAAACCATTAAAGCCATTACTCGATTATAC
TATTTCAAGTAACAAAGAGGCTGCATGTGAATTTGTTACAGAAATAGTACCTATAATAAATAGGGCAAAAAAGCCTGTTA
TTCTTGCAGATTATGGAGTATATCGTTACCAAGTTCAACATGTGCTTAAAAACTTGGCCGAAAAAACCGGATTTCCTGTG
GCTACACTAAGTATGGGAAAAGGTGTTTTCAATGAAGCACACCCTCAATTTATTGGTGTTTATAATGGTGATGTAAGTTC
TCCTTATTTAAGGCAGCGAGTTGATGAAGCAGACTGCATTATTAGCGTTGGTGTAAAATTGACGGATTCAACCACAGGGG
GATTTTCTCATGGATTTTCTAAAAGGAATGTAATTCACATTGATCCTTTTTCAATAAAGGCAAAAGGTAAAAAATATGCA
CCTATTACGATGAAAGATGCTTTAACAGAATTAACAAGTAAAATTGAGCATAGAAACTTTGAGGATTTAGATATAAAGCC
TTACAAATCAGATAATCAAAAGTATTTTGCAAAAGAGAAGCCAATTACACAAAAACGTTTTTTTGAGCGTATTGCTCACT
TTATAAAAGAAAAAGATGTATTATTAGCAGAACAGGGTACATGCTTTTTTGGTGCGTCAACCATACAACTACCCAAAGAT
GCAACTTTTATTGGTCAACCTTTATGGGGATCTATTGGATACACACTTCCTGCTTTATTAGGTTCACAATTAGCTGATCA
AAAAAGGCGTAATATTCTTTTAATTGGGGATGGTGCATTTCAAATGACAGCACAAGAAATTTCAACAATGCTTCGTTTAC
AAATCAAACCTATTATTTTTTTAATTAATAACGATGGTTATACAATTGAACGTGCTATTCATGGTAGAGAACAAGTATAT
AACAATATTCAAATGTGGCGATATCATAATGTTCCAAAGGTTTTAGGTCCTAAAGAATGCAGCTTAACCTTTAAAGTACA
AAGTGAAACTGAACTTGAAAAGGCTCTTTTAGTGGCAGATAAGGATTGTGAACATTTGATTTTTATAGAAGTTGTTATGG
ATCGTTATGATAAACCCGAGCCTTTAGAACGTCTTTCGAAACGTTTTGCAAATCAAAATAATTAG

Upstream 100 bases:

>100_bases
TACATCCTAAAAAGTCAATGTACTTGACTAAAAATGGGACTTGTTATATCATAATAATTAGGTCAACTTAGTTGACCTAA
TTAAGGTGGAGGTGACAATT

Downstream 100 bases:

>100_bases
TTCAAAATATAGCATTTTAAATAGTAATCTTAAGCGTGCATTATAGAATTGAAAATAAAAATTCAGAATATTCTTACTGA
ATCTATCTATAGCCTATTAT

Product: pyruvate decarboxylase

Products: aldehyde; CO2

Alternate protein names: KDC [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKSEYTIGRYLLDRLSELGIRHIFGVPGDYNLSFLDYIMEYKGIDWVGNCNELNAGYAADGYARINGIGAILTTFGVGEL
SAINAIAGAYAEQVPVVKITGIPTAKVRDNGLYVHHTLGDGRFDHFFEMFREVTVAEALLSEENAAQEIDRVLISCWRQK
RPVLINLPIDVYDKPINKPLKPLLDYTISSNKEAACEFVTEIVPIINRAKKPVILADYGVYRYQVQHVLKNLAEKTGFPV
ATLSMGKGVFNEAHPQFIGVYNGDVSSPYLRQRVDEADCIISVGVKLTDSTTGGFSHGFSKRNVIHIDPFSIKAKGKKYA
PITMKDALTELTSKIEHRNFEDLDIKPYKSDNQKYFAKEKPITQKRFFERIAHFIKEKDVLLAEQGTCFFGASTIQLPKD
ATFIGQPLWGSIGYTLPALLGSQLADQKRRNILLIGDGAFQMTAQEISTMLRLQIKPIIFLINNDGYTIERAIHGREQVY
NNIQMWRYHNVPKVLGPKECSLTFKVQSETELEKALLVADKDCEHLIFIEVVMDRYDKPEPLERLSKRFANQNN

Sequences:

>Translated_554_residues
MKSEYTIGRYLLDRLSELGIRHIFGVPGDYNLSFLDYIMEYKGIDWVGNCNELNAGYAADGYARINGIGAILTTFGVGEL
SAINAIAGAYAEQVPVVKITGIPTAKVRDNGLYVHHTLGDGRFDHFFEMFREVTVAEALLSEENAAQEIDRVLISCWRQK
RPVLINLPIDVYDKPINKPLKPLLDYTISSNKEAACEFVTEIVPIINRAKKPVILADYGVYRYQVQHVLKNLAEKTGFPV
ATLSMGKGVFNEAHPQFIGVYNGDVSSPYLRQRVDEADCIISVGVKLTDSTTGGFSHGFSKRNVIHIDPFSIKAKGKKYA
PITMKDALTELTSKIEHRNFEDLDIKPYKSDNQKYFAKEKPITQKRFFERIAHFIKEKDVLLAEQGTCFFGASTIQLPKD
ATFIGQPLWGSIGYTLPALLGSQLADQKRRNILLIGDGAFQMTAQEISTMLRLQIKPIIFLINNDGYTIERAIHGREQVY
NNIQMWRYHNVPKVLGPKECSLTFKVQSETELEKALLVADKDCEHLIFIEVVMDRYDKPEPLERLSKRFANQNN
>Mature_554_residues
MKSEYTIGRYLLDRLSELGIRHIFGVPGDYNLSFLDYIMEYKGIDWVGNCNELNAGYAADGYARINGIGAILTTFGVGEL
SAINAIAGAYAEQVPVVKITGIPTAKVRDNGLYVHHTLGDGRFDHFFEMFREVTVAEALLSEENAAQEIDRVLISCWRQK
RPVLINLPIDVYDKPINKPLKPLLDYTISSNKEAACEFVTEIVPIINRAKKPVILADYGVYRYQVQHVLKNLAEKTGFPV
ATLSMGKGVFNEAHPQFIGVYNGDVSSPYLRQRVDEADCIISVGVKLTDSTTGGFSHGFSKRNVIHIDPFSIKAKGKKYA
PITMKDALTELTSKIEHRNFEDLDIKPYKSDNQKYFAKEKPITQKRFFERIAHFIKEKDVLLAEQGTCFFGASTIQLPKD
ATFIGQPLWGSIGYTLPALLGSQLADQKRRNILLIGDGAFQMTAQEISTMLRLQIKPIIFLINNDGYTIERAIHGREQVY
NNIQMWRYHNVPKVLGPKECSLTFKVQSETELEKALLVADKDCEHLIFIEVVMDRYDKPEPLERLSKRFANQNN

Specific function: Decarboxylates branched-chain and aromatic alpha-keto acids to aldehydes [H]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=24.7349823321555, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1790104, Length=499, Percent_Identity=23.0460921843687, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1786717, Length=477, Percent_Identity=25.9958071278826, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787096, Length=480, Percent_Identity=22.9166666666667, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=565, Percent_Identity=40.8849557522124, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323073, Length=565, Percent_Identity=39.2920353982301, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321524, Length=570, Percent_Identity=38.4210526315789, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6320123, Length=521, Percent_Identity=35.1247600767754, Blast_Score=337, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6320588, Length=613, Percent_Identity=30.6688417618271, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323755, Length=497, Percent_Identity=26.5593561368209, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 4.1.1.1

Molecular weight: Translated: 62562; Mature: 62562

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEYTIGRYLLDRLSELGIRHIFGVPGDYNLSFLDYIMEYKGIDWVGNCNELNAGYAAD
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GYARINGIGAILTTFGVGELSAINAIAGAYAEQVPVVKITGIPTAKVRDNGLYVHHTLGD
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEEECCC
GRFDHFFEMFREVTVAEALLSEENAAQEIDRVLISCWRQKRPVLINLPIDVYDKPINKPL
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHH
KPLLDYTISSNKEAACEFVTEIVPIINRAKKPVILADYGVYRYQVQHVLKNLAEKTGFPV
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCE
ATLSMGKGVFNEAHPQFIGVYNGDVSSPYLRQRVDEADCIISVGVKLTDSTTGGFSHGFS
EEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEECEEEECCCCCCCCCCCC
KRNVIHIDPFSIKAKGKKYAPITMKDALTELTSKIEHRNFEDLDIKPYKSDNQKYFAKEK
CCCEEEECCCEEEECCCEECCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCC
PITQKRFFERIAHFIKEKDVLLAEQGTCFFGASTIQLPKDATFIGQPLWGSIGYTLPALL
CCHHHHHHHHHHHHHCCCCEEEECCCCEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHH
GSQLADQKRRNILLIGDGAFQMTAQEISTMLRLQIKPIIFLINNDGYTIERAIHGREQVY
HHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEHHHCCHHHHH
NNIQMWRYHNVPKVLGPKECSLTFKVQSETELEKALLVADKDCEHLIFIEVVMDRYDKPE
CCHHEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHEECCCCCCEEHHHHHHHHCCCCC
PLERLSKRFANQNN
HHHHHHHHHCCCCC
>Mature Secondary Structure
MKSEYTIGRYLLDRLSELGIRHIFGVPGDYNLSFLDYIMEYKGIDWVGNCNELNAGYAAD
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GYARINGIGAILTTFGVGELSAINAIAGAYAEQVPVVKITGIPTAKVRDNGLYVHHTLGD
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEEECCC
GRFDHFFEMFREVTVAEALLSEENAAQEIDRVLISCWRQKRPVLINLPIDVYDKPINKPL
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHH
KPLLDYTISSNKEAACEFVTEIVPIINRAKKPVILADYGVYRYQVQHVLKNLAEKTGFPV
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCE
ATLSMGKGVFNEAHPQFIGVYNGDVSSPYLRQRVDEADCIISVGVKLTDSTTGGFSHGFS
EEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEECEEEECCCCCCCCCCCC
KRNVIHIDPFSIKAKGKKYAPITMKDALTELTSKIEHRNFEDLDIKPYKSDNQKYFAKEK
CCCEEEECCCEEEECCCEECCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCC
PITQKRFFERIAHFIKEKDVLLAEQGTCFFGASTIQLPKDATFIGQPLWGSIGYTLPALL
CCHHHHHHHHHHHHHCCCCEEEECCCCEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHH
GSQLADQKRRNILLIGDGAFQMTAQEISTMLRLQIKPIIFLINNDGYTIERAIHGREQVY
HHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEHHHCCHHHHH
NNIQMWRYHNVPKVLGPKECSLTFKVQSETELEKALLVADKDCEHLIFIEVVMDRYDKPE
CCHHEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHEECCCCCCEEHHHHHHHHCCCCC
PLERLSKRFANQNN
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-oxo acid

Specific reaction: A 2-oxo acid = an aldehyde + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]