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The map label for this gene is glnA [H]

Identifier: 150010482

GI number: 150010482

Start: 4753176

End: 4754678

Strand: Direct

Name: glnA [H]

Synonym: BDI_3923

Alternate gene names: 150010482

Gene position: 4753176-4754678 (Clockwise)

Preceding gene: 150010481

Following gene: 150010483

Centisome position: 98.79

GC content: 44.64

Gene sequence:

>1503_bases
ATGAACAAAGAACTAGAAATGACTCCCAACGTATTAGTGGAGTTTTTACAAAAGCCAGCGTCCGAGTTTACGAAAGAGGA
CATTATCCGTTATATCTATGAGAAAGATATCCAGATGGTTAATTTTATGTATCCTGCAGGCGACGGACGTTTGAAAACAC
TGAACTTTGTAATCAACAATGCAGCTTATCTGGATGCGATCTTGACTTGCGGTGAGCGTGTGGATGGCTCTAGTCTTTTC
TCGTTTATCGAGGCGGGGAGTAGTGACCTGTATGTGGTGCCCCGTTTCCGTACGGCTTTCGAGGATCCGTTTGCGGAATT
ACCTACGCTTACCATGCTTTGCTCATTCTTCAATAAGGATGGAGAACCCTTAGAAAGTTCCCCGGAATATACCTTGCATA
AAGCTTGTAAGGCTTTTCATGAGGTGACGGGAATGGATTTTGAGGCAATGGGTGAATTAGAATATTACGTAATCGCACCG
GATGAAGGCGTTTTCCCTGCTACCGACCAAAAAGGTTATCATGAATCCGCTCCTTACGCTAAGTTCAATGAGTTCCGGAC
GGAGTGCATGCACTGCATAGCGCAAGCCGGGGGACAAATTAAATATGGTCATTCGGAAGTTGGTAACTTTACGTTAAATG
GTTTGATCTATGAGCAAAATGAGATCGAGTTCTTACCATGTCCTGCGGAAAATGCAGCGGATCAATTGATGATCGCAAAA
TGGGCGATCCGCAATCTAGCGCATGAATATGGATATAACATCACTTTCGCTCCGAAAATTACGGTGGGAAAAGCAGGTTC
CGGTCTGCATGTCCATATGCGTATCATGAAAAATGGCAAGAATCAGATGTTAGCGAATGGTATCTTATCCGAAACCGCCC
GTAAGGCTATCGCTGGAATGATGTGCTTAGCTTCCTCTATTACGGCTTTTGGAAATACGAACCCAACCTCATATTTCCGC
TTGGTTCCACACCAAGAAGCACCTACGAATATTTGTTGGGGAGATCGGAATCGTTCAGTTTTAGTTCGTGTTCCGCTAGG
TTGGGCCGCAAAAACGGACATGTGTGCTTTGGCGAACCCATTGGAACCTGCCAGCCATTATGATACGAGCCAAAAGCAAA
CGGTCGAGATGCGTTCACCGGATGGCTCCGCTGATTTATACCAATTATTGGCAGGATTGGCAGTTGCGTGCCGCCATGGT
TTTGAGATCGATGATGCTTTGGAAATCGCTGAGAAGACTTATGTAAACGTGAATATTCATAAAGAAGAGAATCAAGATCG
CCTAAAGACATTGGATCAACTCCCAGACAGTTGCGCAGCCTCAGCCGCCCGTTTAAGACAACAGCGTGCTGTTTTTGAAA
AATATAACGTATTTAGTCCGAGTATGATAGACGGCATCATCCATAAACTGGAATCTTATGATGACGCTTCTTTACGCAAG
GACCTAGAGAATAATCCGGAAGAGATGTTGAAGCTGGTCAATACTTATTTCCATTGTGGCTGA

Upstream 100 bases:

>100_bases
CCGACCTATAGAGGTTGCTGAAGCGATCTCTAGCTTGATTCCGGTTAGCAGTTCCAAAGGAAAGTTTTTCTCTACACTAT
AAACATAATCAATATAAAAC

Downstream 100 bases:

>100_bases
AAATCTTTTTTATGGTTTCACAAGAGGGTGTGTCATGACACACCCTTTTTATGCCCTGCTTTCATTTCTTGAACTTTCCA
TTGCGTTTTTTTTGAATATC

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKELEMTPNVLVEFLQKPASEFTKEDIIRYIYEKDIQMVNFMYPAGDGRLKTLNFVINNAAYLDAILTCGERVDGSSLF
SFIEAGSSDLYVVPRFRTAFEDPFAELPTLTMLCSFFNKDGEPLESSPEYTLHKACKAFHEVTGMDFEAMGELEYYVIAP
DEGVFPATDQKGYHESAPYAKFNEFRTECMHCIAQAGGQIKYGHSEVGNFTLNGLIYEQNEIEFLPCPAENAADQLMIAK
WAIRNLAHEYGYNITFAPKITVGKAGSGLHVHMRIMKNGKNQMLANGILSETARKAIAGMMCLASSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCALANPLEPASHYDTSQKQTVEMRSPDGSADLYQLLAGLAVACRHG
FEIDDALEIAEKTYVNVNIHKEENQDRLKTLDQLPDSCAASAARLRQQRAVFEKYNVFSPSMIDGIIHKLESYDDASLRK
DLENNPEEMLKLVNTYFHCG

Sequences:

>Translated_500_residues
MNKELEMTPNVLVEFLQKPASEFTKEDIIRYIYEKDIQMVNFMYPAGDGRLKTLNFVINNAAYLDAILTCGERVDGSSLF
SFIEAGSSDLYVVPRFRTAFEDPFAELPTLTMLCSFFNKDGEPLESSPEYTLHKACKAFHEVTGMDFEAMGELEYYVIAP
DEGVFPATDQKGYHESAPYAKFNEFRTECMHCIAQAGGQIKYGHSEVGNFTLNGLIYEQNEIEFLPCPAENAADQLMIAK
WAIRNLAHEYGYNITFAPKITVGKAGSGLHVHMRIMKNGKNQMLANGILSETARKAIAGMMCLASSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCALANPLEPASHYDTSQKQTVEMRSPDGSADLYQLLAGLAVACRHG
FEIDDALEIAEKTYVNVNIHKEENQDRLKTLDQLPDSCAASAARLRQQRAVFEKYNVFSPSMIDGIIHKLESYDDASLRK
DLENNPEEMLKLVNTYFHCG
>Mature_500_residues
MNKELEMTPNVLVEFLQKPASEFTKEDIIRYIYEKDIQMVNFMYPAGDGRLKTLNFVINNAAYLDAILTCGERVDGSSLF
SFIEAGSSDLYVVPRFRTAFEDPFAELPTLTMLCSFFNKDGEPLESSPEYTLHKACKAFHEVTGMDFEAMGELEYYVIAP
DEGVFPATDQKGYHESAPYAKFNEFRTECMHCIAQAGGQIKYGHSEVGNFTLNGLIYEQNEIEFLPCPAENAADQLMIAK
WAIRNLAHEYGYNITFAPKITVGKAGSGLHVHMRIMKNGKNQMLANGILSETARKAIAGMMCLASSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCALANPLEPASHYDTSQKQTVEMRSPDGSADLYQLLAGLAVACRHG
FEIDDALEIAEKTYVNVNIHKEENQDRLKTLDQLPDSCAASAARLRQQRAVFEKYNVFSPSMIDGIIHKLESYDDASLRK
DLENNPEEMLKLVNTYFHCG

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=490, Percent_Identity=25.3061224489796, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081870, Length=352, Percent_Identity=25.8522727272727, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 56008; Mature: 56008

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELEMTPNVLVEFLQKPASEFTKEDIIRYIYEKDIQMVNFMYPAGDGRLKTLNFVINN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEECCC
AAYLDAILTCGERVDGSSLFSFIEAGSSDLYVVPRFRTAFEDPFAELPTLTMLCSFFNKD
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCC
GEPLESSPEYTLHKACKAFHEVTGMDFEAMGELEYYVIAPDEGVFPATDQKGYHESAPYA
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
KFNEFRTECMHCIAQAGGQIKYGHSEVGNFTLNGLIYEQNEIEFLPCPAENAADQLMIAK
HHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHHH
WAIRNLAHEYGYNITFAPKITVGKAGSGLHVHMRIMKNGKNQMLANGILSETARKAIAGM
HHHHHHHHHHCCCEEEECEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
MCLASSITAFGNTNPTSYFRLVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCALANP
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEECCCCCEEEEEECCCCCCCCCHHHHCCC
LEPASHYDTSQKQTVEMRSPDGSADLYQLLAGLAVACRHGFEIDDALEIAEKTYVNVNIH
CCCCHHCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEE
KEENQDRLKTLDQLPDSCAASAARLRQQRAVFEKYNVFSPSMIDGIIHKLESYDDASLRK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHH
DLENNPEEMLKLVNTYFHCG
HHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKELEMTPNVLVEFLQKPASEFTKEDIIRYIYEKDIQMVNFMYPAGDGRLKTLNFVINN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEECCC
AAYLDAILTCGERVDGSSLFSFIEAGSSDLYVVPRFRTAFEDPFAELPTLTMLCSFFNKD
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCC
GEPLESSPEYTLHKACKAFHEVTGMDFEAMGELEYYVIAPDEGVFPATDQKGYHESAPYA
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
KFNEFRTECMHCIAQAGGQIKYGHSEVGNFTLNGLIYEQNEIEFLPCPAENAADQLMIAK
HHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHHH
WAIRNLAHEYGYNITFAPKITVGKAGSGLHVHMRIMKNGKNQMLANGILSETARKAIAGM
HHHHHHHHHHCCCEEEECEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
MCLASSITAFGNTNPTSYFRLVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCALANP
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEECCCCCEEEEEECCCCCCCCCHHHHCCC
LEPASHYDTSQKQTVEMRSPDGSADLYQLLAGLAVACRHGFEIDDALEIAEKTYVNVNIH
CCCCHHCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEE
KEENQDRLKTLDQLPDSCAASAARLRQQRAVFEKYNVFSPSMIDGIIHKLESYDDASLRK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHH
DLENNPEEMLKLVNTYFHCG
HHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099074 [H]