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The map label for this gene is gacS [H]

Identifier: 150010361

GI number: 150010361

Start: 4627121

End: 4629091

Strand: Direct

Name: gacS [H]

Synonym: BDI_3796

Alternate gene names: 150010361

Gene position: 4627121-4629091 (Clockwise)

Preceding gene: 150010360

Following gene: 150010362

Centisome position: 96.17

GC content: 42.52

Gene sequence:

>1971_bases
ATGAAGAGATTGTTTTTTATTGGTTGTTGGACGTTGATTCTAACTTTGCTTATTCCGGATAGGGCGAAAGGTGACACGTT
TGTCGATAGCTTACGAAGAGAAATCAAGGTTTTGCCGGATAGTAGTAAACTAATACGCTTGAACGAATTACTTTATGCCA
ATACGCATAACAAAGTATATAAAGTATATGCGGATTTGCTACTAGAAGAGGCACAGAGGCAACGTAACGATTATTACAAA
GGAAATGCCTTACTATTCTTGATGCGTTATTACTATATGCAAGATCCGGATAGCTTGCGAATATATTTGAAAATAGCGGA
ACCCCTTTTCATCGCGACAAACCGGATCGAGGAATTATGTAGGGCGAAAGGATGGAATATCTATTCTTTAGCGAATGAGG
GGATGCAGGGATTGGTAATCCGGGAGGTGGATAGCTTGAGGAATCTTGCGACCTGTTTTAATTATCCGGATGGGGTGGAT
ATGGCAAACCAAGCTTTAGCGAATTTCTATTTTAATATAGGATTGGATGAGGAGGGTATTCAATTAAGCCGGGAGATCTT
ATCTCGCATGGAAGAACGTAATGCCTCTAGGATGCGTTGGTATTACGTGTTACGGCTGTTGCTTACGAAGGATTTGCGAT
TGGAGTATTTGAATAAATTGGATTCTTGCATACAAGAGTGCGAGAAAGAGGGCATTACTCAATTGGATGCGGAACATACT
GTCGCTTTTTTAAAAGATCGCTATCATTATTACTCGGCCCAGTATTACGTGGCGGAAAAAGAGCCTTCCTTGGTTTATCA
GCACTTGAGGATGATGGAGGATATCGAAAAGAAGAATAATATGAATGCCGAGCAAATCCTGCCTCAATTCCTTTGGATGA
ATTATTACGCTTTAGCGGGGAAATATGAGAAGGCCATTGATTTGGCGAACAAATTGGAACTAATGCTCTTGGATAAGAAA
CGCTTTAATGATTGGGTCTCAGTGGAGGATTTTAAGGCGGATCTCTATTATAAGCTTGGAAGAGGGATGGAAGCGGCTAG
GGCTTACAGAGATTCTAAAGAAGTGCACGACTCGATTATGCGGGTAAAATATTACGAGGATTTAGCGAAGTTGAAAACCC
AACGAGAGGTGGAAAAACTAGAGATACAAAGTAAGAAACTGGAGCTAGAGGCGGAAAAGTCGCGTGTCCGTATCTTGATG
CTTCGAGGAGGATTTGTCTTGGTGTTGTTTCTATGTGCGGGCTTGGGTATAGTCGCCTATGCCCGTCATCGTGCGGGGAT
ACGATTAAAGATCGCAAAAGAGAAAGCGGAGGAGGCGGATCACCTGAAATCTGCGTTCTTGGCAAACATGAATCATGAGA
TCAGGACGCCATTGAACGCCATCGTCGGATTCTCGCAGGTGATCGCCGATGAGGAAGATGCGGAAACTCGTCATGAACTC
TCCAATATTATACAAAGTAATAATGAATTGTTGCAACGCTTGATAGAAGACGTATTGGATATATCGAAAATCGAGTCGAA
TACGTTGACTTTCGTTTTGGCGAATCATGAAATGAAGGCTTTAATGAAAGATATCTATAGTATCATTTTATTACGTATGC
CCGAAAATGTAGAACTTCGATTGGATGATTGCCAACCTTTTACGCTTTATACCGATCGGAGTAGATTAACGCAAGTCCTC
ACTAATTTATTGACGAATGCTATCAAGCACACGAAAAAAGGTTATATTTGCTTCGGGTATGACGTGACGGAGCAGGAAAT
CCGCTTTTATGTGACGGATACAGGCGAGGGAATCCCGGACGACCAATTGGAACGGGTTTTCGATCGTTTTGTCAAGTTGA
CGCAATGGACCAATGGAGTCGGCTTGGGATTGGCTATCTCCAAGGCTTTGGTCACAAAGCTCGGCGGTCGTATAGAAGTG
ACTTCCCAGCAAGGGGTAGGCTCTACCTTTAGCGTTATTTTCCCAAGATAA

Upstream 100 bases:

>100_bases
TGATATTAAACTTGTTTATGTTGATGTCTTGGCATTTAAATTTTTATTAACTTGCCGGGCATTGAATAATGGATAGTCGT
GCTAACTTTTCAAGGTATAT

Downstream 100 bases:

>100_bases
ACAGTATACATTATATATAATATTGTAATAGACCATGAAACACTCAAGACGTACATTTTTTAAACAAGGATTGGCGGGTG
CCCTTTTATTAGGTACCTCC

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MKRLFFIGCWTLILTLLIPDRAKGDTFVDSLRREIKVLPDSSKLIRLNELLYANTHNKVYKVYADLLLEEAQRQRNDYYK
GNALLFLMRYYYMQDPDSLRIYLKIAEPLFIATNRIEELCRAKGWNIYSLANEGMQGLVIREVDSLRNLATCFNYPDGVD
MANQALANFYFNIGLDEEGIQLSREILSRMEERNASRMRWYYVLRLLLTKDLRLEYLNKLDSCIQECEKEGITQLDAEHT
VAFLKDRYHYYSAQYYVAEKEPSLVYQHLRMMEDIEKKNNMNAEQILPQFLWMNYYALAGKYEKAIDLANKLELMLLDKK
RFNDWVSVEDFKADLYYKLGRGMEAARAYRDSKEVHDSIMRVKYYEDLAKLKTQREVEKLEIQSKKLELEAEKSRVRILM
LRGGFVLVLFLCAGLGIVAYARHRAGIRLKIAKEKAEEADHLKSAFLANMNHEIRTPLNAIVGFSQVIADEEDAETRHEL
SNIIQSNNELLQRLIEDVLDISKIESNTLTFVLANHEMKALMKDIYSIILLRMPENVELRLDDCQPFTLYTDRSRLTQVL
TNLLTNAIKHTKKGYICFGYDVTEQEIRFYVTDTGEGIPDDQLERVFDRFVKLTQWTNGVGLGLAISKALVTKLGGRIEV
TSQQGVGSTFSVIFPR

Sequences:

>Translated_656_residues
MKRLFFIGCWTLILTLLIPDRAKGDTFVDSLRREIKVLPDSSKLIRLNELLYANTHNKVYKVYADLLLEEAQRQRNDYYK
GNALLFLMRYYYMQDPDSLRIYLKIAEPLFIATNRIEELCRAKGWNIYSLANEGMQGLVIREVDSLRNLATCFNYPDGVD
MANQALANFYFNIGLDEEGIQLSREILSRMEERNASRMRWYYVLRLLLTKDLRLEYLNKLDSCIQECEKEGITQLDAEHT
VAFLKDRYHYYSAQYYVAEKEPSLVYQHLRMMEDIEKKNNMNAEQILPQFLWMNYYALAGKYEKAIDLANKLELMLLDKK
RFNDWVSVEDFKADLYYKLGRGMEAARAYRDSKEVHDSIMRVKYYEDLAKLKTQREVEKLEIQSKKLELEAEKSRVRILM
LRGGFVLVLFLCAGLGIVAYARHRAGIRLKIAKEKAEEADHLKSAFLANMNHEIRTPLNAIVGFSQVIADEEDAETRHEL
SNIIQSNNELLQRLIEDVLDISKIESNTLTFVLANHEMKALMKDIYSIILLRMPENVELRLDDCQPFTLYTDRSRLTQVL
TNLLTNAIKHTKKGYICFGYDVTEQEIRFYVTDTGEGIPDDQLERVFDRFVKLTQWTNGVGLGLAISKALVTKLGGRIEV
TSQQGVGSTFSVIFPR
>Mature_656_residues
MKRLFFIGCWTLILTLLIPDRAKGDTFVDSLRREIKVLPDSSKLIRLNELLYANTHNKVYKVYADLLLEEAQRQRNDYYK
GNALLFLMRYYYMQDPDSLRIYLKIAEPLFIATNRIEELCRAKGWNIYSLANEGMQGLVIREVDSLRNLATCFNYPDGVD
MANQALANFYFNIGLDEEGIQLSREILSRMEERNASRMRWYYVLRLLLTKDLRLEYLNKLDSCIQECEKEGITQLDAEHT
VAFLKDRYHYYSAQYYVAEKEPSLVYQHLRMMEDIEKKNNMNAEQILPQFLWMNYYALAGKYEKAIDLANKLELMLLDKK
RFNDWVSVEDFKADLYYKLGRGMEAARAYRDSKEVHDSIMRVKYYEDLAKLKTQREVEKLEIQSKKLELEAEKSRVRILM
LRGGFVLVLFLCAGLGIVAYARHRAGIRLKIAKEKAEEADHLKSAFLANMNHEIRTPLNAIVGFSQVIADEEDAETRHEL
SNIIQSNNELLQRLIEDVLDISKIESNTLTFVLANHEMKALMKDIYSIILLRMPENVELRLDDCQPFTLYTDRSRLTQVL
TNLLTNAIKHTKKGYICFGYDVTEQEIRFYVTDTGEGIPDDQLERVFDRFVKLTQWTNGVGLGLAISKALVTKLGGRIEV
TSQQGVGSTFSVIFPR

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=35.4430379746835, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=34.4978165938865, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=34.9344978165939, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=31.7180616740088, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=29.8319327731092, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=28.8135593220339, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=28, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=26.2222222222222, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=28.310502283105, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=203, Percent_Identity=29.064039408867, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=28.3898305084746, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=245, Percent_Identity=24.4897959183673, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76370; Mature: 76370

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFFIGCWTLILTLLIPDRAKGDTFVDSLRREIKVLPDSSKLIRLNELLYANTHNKVY
CCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCEEEEHHHHEECCCHHHH
KVYADLLLEEAQRQRNDYYKGNALLFLMRYYYMQDPDSLRIYLKIAEPLFIATNRIEELC
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
RAKGWNIYSLANEGMQGLVIREVDSLRNLATCFNYPDGVDMANQALANFYFNIGLDEEGI
HHCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCHHHH
QLSREILSRMEERNASRMRWYYVLRLLLTKDLRLEYLNKLDSCIQECEKEGITQLDAEHT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VAFLKDRYHYYSAQYYVAEKEPSLVYQHLRMMEDIEKKNNMNAEQILPQFLWMNYYALAG
HHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KYEKAIDLANKLELMLLDKKRFNDWVSVEDFKADLYYKLGRGMEAARAYRDSKEVHDSIM
CHHHHHHHHHHHHEEEECCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RVKYYEDLAKLKTQREVEKLEIQSKKLELEAEKSRVRILMLRGGFVLVLFLCAGLGIVAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHHHHHHHHHHHHHH
ARHRAGIRLKIAKEKAEEADHLKSAFLANMNHEIRTPLNAIVGFSQVIADEEDAETRHEL
HHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHH
SNIIQSNNELLQRLIEDVLDISKIESNTLTFVLANHEMKALMKDIYSIILLRMPENVELR
HHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEE
LDDCQPFTLYTDRSRLTQVLTNLLTNAIKHTKKGYICFGYDVTEQEIRFYVTDTGEGIPD
ECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCH
DQLERVFDRFVKLTQWTNGVGLGLAISKALVTKLGGRIEVTSQQGVGSTFSVIFPR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC
>Mature Secondary Structure
MKRLFFIGCWTLILTLLIPDRAKGDTFVDSLRREIKVLPDSSKLIRLNELLYANTHNKVY
CCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCEEEEHHHHEECCCHHHH
KVYADLLLEEAQRQRNDYYKGNALLFLMRYYYMQDPDSLRIYLKIAEPLFIATNRIEELC
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
RAKGWNIYSLANEGMQGLVIREVDSLRNLATCFNYPDGVDMANQALANFYFNIGLDEEGI
HHCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCHHHH
QLSREILSRMEERNASRMRWYYVLRLLLTKDLRLEYLNKLDSCIQECEKEGITQLDAEHT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VAFLKDRYHYYSAQYYVAEKEPSLVYQHLRMMEDIEKKNNMNAEQILPQFLWMNYYALAG
HHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KYEKAIDLANKLELMLLDKKRFNDWVSVEDFKADLYYKLGRGMEAARAYRDSKEVHDSIM
CHHHHHHHHHHHHEEEECCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RVKYYEDLAKLKTQREVEKLEIQSKKLELEAEKSRVRILMLRGGFVLVLFLCAGLGIVAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHHHHHHHHHHHHHH
ARHRAGIRLKIAKEKAEEADHLKSAFLANMNHEIRTPLNAIVGFSQVIADEEDAETRHEL
HHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHH
SNIIQSNNELLQRLIEDVLDISKIESNTLTFVLANHEMKALMKDIYSIILLRMPENVELR
HHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEE
LDDCQPFTLYTDRSRLTQVLTNLLTNAIKHTKKGYICFGYDVTEQEIRFYVTDTGEGIPD
ECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCH
DQLERVFDRFVKLTQWTNGVGLGLAISKALVTKLGGRIEVTSQQGVGSTFSVIFPR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]