Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 150010350

GI number: 150010350

Start: 4613150

End: 4614505

Strand: Direct

Name: hisS

Synonym: BDI_3784

Alternate gene names: 150010350

Gene position: 4613150-4614505 (Clockwise)

Preceding gene: 150010349

Following gene: 150010351

Centisome position: 95.88

GC content: 45.5

Gene sequence:

>1356_bases
ATGCAAAAACCTTCCATACCCAAAGGTACTCGGGATTTTTCGCCGGAGGAAATGGCGAAACGTAATTATATATTCAACAC
GATCCGTGAAGTATTTCACTTGTATGGCTTCCAGCAAATAGAGACTCCTGCCATGGAGAACCTTTCTACATTGATGGGTA
AATATGGCGAGGAAGGGGATAAGCTCCTTTTCAAGATATTGAATTCCGGTGATTGTTTCGCTGGTATCTCGGATGAGGAG
TTAATGGAAAAGAACCCGTTGAGATTTGCTGCTAAGGCTTGTGAGAAAGGCTTGCGTTATGATTTGACCGTTCCGTTCGC
CCGTTACGTGGTACAACATCGTAATGAGATCAGTTTTCCGTTCAAACGTTACCAGATCCAACCGGTTTGGCGTGCCGATC
GTCCGCAAAAGGGACGTTACCGGGAGTTCTACCAATGTGATGGCGACGTTGTGGGTTCTGATTCCTTGATCAATGAGGTG
GAACTGATCCAGATTATGGACGAGGTGTTCCATCGTTTTGGCATTCGTGTGTGTATCAAGATGAACAATCGTAAGATCCT
TTCCGGTATTGCCGAAATCATAGGCGAGGCGGATAAGATCGTAGACATTACGGTAGCTATCGACAAGCTGGATAAGATTG
GCTTGGACAACGTGAATGAAGAGCTTCGTTCCAAGGGTTTACCCGAGGAGGCGATCGAGCGTTTGCAGCCGATCATTATG
CTGCAGGGTTCAAATCAAGAAAAGATCGCTACCTTGAAAAAGGTATTATCTGCGTCTGAGACTGGTTTGAAAGGTGTAGC
CGAATTGGATTTTATTTTGGAGCGCATCTCTCATACGTCAATCCGTGCGGAATTAGAGCTTGATCTGACATTAGCTCGCG
GCTTAAACTATTATACAGGTGCGATTTTCGAGGTGAAAGCCTTGGACGTACAGATCGGTAGTATCACGGGTGGTGGTCGC
TATGACAACCTGACAGGGGTATTTGGCATGGAAGGCGTATCTGGTGTCGGTTTCTCTTTCGGCGCTGATCGTATCTTTGA
TGTATTGAACCAATTGGATTTATATCCGGAAGGATCTTTGAAGACTACACAGCTTCTATTCGTGAACTTCGGACAGAAAG
AGGAGATTCATTTATTGCCGCTGATCGCTAAGGTTCGTAAGGCGGGGATTCGTACAGAGCTATATCCTGAGTCTACTAAG
ATGAAAAAGCAAATGGGATATGCGGACGCTAAGAAAATCCCGTTCGTTGCGATAGTCGGAGAGAATGAGATGGCCGAGAA
TAAAATCAACTTAAAGAATATGCTCACGGGTGAGCAAACCCTTGTGACTATCGAGGAGCTGATAGAACGCTTTTAG

Upstream 100 bases:

>100_bases
CGATTGTAAGCTCCTTACAGTGGGCATTGTTATTTTCTTATTTATTTCGAGCAAAAAACATTACCTTTGCGGCCAGAATA
ATCATTTAAAACATAAGATC

Downstream 100 bases:

>100_bases
AGAATAGTGGATTATGGAACACGGACTACACGGATGACACGGTATTTGTATTTATCCGTGTGTTCCGTGTCATCCGTGTA
GTCCGTGTTTAAATGGTTCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQKPSIPKGTRDFSPEEMAKRNYIFNTIREVFHLYGFQQIETPAMENLSTLMGKYGEEGDKLLFKILNSGDCFAGISDEE
LMEKNPLRFAAKACEKGLRYDLTVPFARYVVQHRNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDGDVVGSDSLINEV
ELIQIMDEVFHRFGIRVCIKMNNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLDNVNEELRSKGLPEEAIERLQPIIM
LQGSNQEKIATLKKVLSASETGLKGVAELDFILERISHTSIRAELELDLTLARGLNYYTGAIFEVKALDVQIGSITGGGR
YDNLTGVFGMEGVSGVGFSFGADRIFDVLNQLDLYPEGSLKTTQLLFVNFGQKEEIHLLPLIAKVRKAGIRTELYPESTK
MKKQMGYADAKKIPFVAIVGENEMAENKINLKNMLTGEQTLVTIEELIERF

Sequences:

>Translated_451_residues
MQKPSIPKGTRDFSPEEMAKRNYIFNTIREVFHLYGFQQIETPAMENLSTLMGKYGEEGDKLLFKILNSGDCFAGISDEE
LMEKNPLRFAAKACEKGLRYDLTVPFARYVVQHRNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDGDVVGSDSLINEV
ELIQIMDEVFHRFGIRVCIKMNNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLDNVNEELRSKGLPEEAIERLQPIIM
LQGSNQEKIATLKKVLSASETGLKGVAELDFILERISHTSIRAELELDLTLARGLNYYTGAIFEVKALDVQIGSITGGGR
YDNLTGVFGMEGVSGVGFSFGADRIFDVLNQLDLYPEGSLKTTQLLFVNFGQKEEIHLLPLIAKVRKAGIRTELYPESTK
MKKQMGYADAKKIPFVAIVGENEMAENKINLKNMLTGEQTLVTIEELIERF
>Mature_451_residues
MQKPSIPKGTRDFSPEEMAKRNYIFNTIREVFHLYGFQQIETPAMENLSTLMGKYGEEGDKLLFKILNSGDCFAGISDEE
LMEKNPLRFAAKACEKGLRYDLTVPFARYVVQHRNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDGDVVGSDSLINEV
ELIQIMDEVFHRFGIRVCIKMNNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLDNVNEELRSKGLPEEAIERLQPIIM
LQGSNQEKIATLKKVLSASETGLKGVAELDFILERISHTSIRAELELDLTLARGLNYYTGAIFEVKALDVQIGSITGGGR
YDNLTGVFGMEGVSGVGFSFGADRIFDVLNQLDLYPEGSLKTTQLLFVNFGQKEEIHLLPLIAKVRKAGIRTELYPESTK
MKKQMGYADAKKIPFVAIVGENEMAENKINLKNMLTGEQTLVTIEELIERF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=465, Percent_Identity=37.2043010752688, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI15029520, Length=464, Percent_Identity=34.051724137931, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI65287717, Length=357, Percent_Identity=25.2100840336134, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788861, Length=450, Percent_Identity=25.1111111111111, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71993686, Length=479, Percent_Identity=34.446764091858, Blast_Score=227, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI71993693, Length=476, Percent_Identity=34.0336134453782, Blast_Score=227, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6325290, Length=473, Percent_Identity=34.46088794926, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=33.8235294117647, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24643059, Length=495, Percent_Identity=32.5252525252525, Blast_Score=215, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PARD8 (A6LIF7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001305093.1
- ProteinModelPortal:   A6LIF7
- SMR:   A6LIF7
- STRING:   A6LIF7
- GeneID:   5308933
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3784
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   LERISHT
- ProtClustDB:   PRK00037
- BioCyc:   PDIS435591:BDI_3784-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 50976; Mature: 50976

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPSIPKGTRDFSPEEMAKRNYIFNTIREVFHLYGFQQIETPAMENLSTLMGKYGEEGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCHH
KLLFKILNSGDCFAGISDEELMEKNPLRFAAKACEKGLRYDLTVPFARYVVQHRNEISFP
HHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCC
FKRYQIQPVWRADRPQKGRYREFYQCDGDVVGSDSLINEVELIQIMDEVFHRFGIRVCIK
HHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
MNNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLDNVNEELRSKGLPEEAIERLQPIIM
ECCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCEEE
LQGSNQEKIATLKKVLSASETGLKGVAELDFILERISHTSIRAELELDLTLARGLNYYTG
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEHHCCCCHHCC
AIFEVKALDVQIGSITGGGRYDNLTGVFGMEGVSGVGFSFGADRIFDVLNQLDLYPEGSL
CEEEEEEEEEEEECEECCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
KTTQLLFVNFGQKEEIHLLPLIAKVRKAGIRTELYPESTKMKKQMGYADAKKIPFVAIVG
CEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEC
ENEMAENKINLKNMLTGEQTLVTIEELIERF
CCCHHHCCCCHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MQKPSIPKGTRDFSPEEMAKRNYIFNTIREVFHLYGFQQIETPAMENLSTLMGKYGEEGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCHH
KLLFKILNSGDCFAGISDEELMEKNPLRFAAKACEKGLRYDLTVPFARYVVQHRNEISFP
HHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCC
FKRYQIQPVWRADRPQKGRYREFYQCDGDVVGSDSLINEVELIQIMDEVFHRFGIRVCIK
HHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
MNNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLDNVNEELRSKGLPEEAIERLQPIIM
ECCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCEEE
LQGSNQEKIATLKKVLSASETGLKGVAELDFILERISHTSIRAELELDLTLARGLNYYTG
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEHHCCCCHHCC
AIFEVKALDVQIGSITGGGRYDNLTGVFGMEGVSGVGFSFGADRIFDVLNQLDLYPEGSL
CEEEEEEEEEEEECEECCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
KTTQLLFVNFGQKEEIHLLPLIAKVRKAGIRTELYPESTKMKKQMGYADAKKIPFVAIVG
CEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEC
ENEMAENKINLKNMLTGEQTLVTIEELIERF
CCCHHHCCCCHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA