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The map label for this gene is topA [H]

Identifier: 150010176

GI number: 150010176

Start: 4408880

End: 4411228

Strand: Direct

Name: topA [H]

Synonym: BDI_3599

Alternate gene names: 150010176

Gene position: 4408880-4411228 (Clockwise)

Preceding gene: 150010174

Following gene: 150010180

Centisome position: 91.63

GC content: 48.62

Gene sequence:

>2349_bases
ATGCAAAAGAATTTGGTAATAGTGGAGTCACCCGCCAAGGCAAAAACCATCGAGAAGTTCCTCGGAAAGGACTATAAGGT
TATGTCTAGCTATGGTCATATCCGCGACTTGAAAACGAAAGATTTCAGTATAGACCTCGAACACAATTATGCCCCGGAAT
ACGTGATTCCCTCTGATAAGAAGAAACTCGTGACCGAATTGAAGAACGAAGCCAAGAAAGCCGAGCAGGTTTGGTTGGCT
TCCGATGAGGACCGGGAGGGAGAGGCCATCTCTTGGCATTTATATGAGGTATTAGGATTGAAACCTGAGAATACGAAACG
TATCGTTTTTCATGAAATTACCAAGAACGCTATATTACATGCGATCGAGACGCCGAGAGATATCAATATCGATTTGGTGA
ACGCGCAGCAAGCCCGTCGTATCTTAGACCGTATCGTGGGTTTCGAGCTATCCCCCATTCTTTGGCGTAAGGTGAAGCCG
GCTTTATCCGCGGGGCGCGTGCAATCGGTTGCCGTACGTCTGATCGTAGAGCGGGAGCGAGAGATCAACGAGTTTGTCTC
GGAGGCCGCCTACCGTGTGATCGCTAGCTTTATCTTGCCGGATGGTACGACAATCCTCAAGGCGGAGCTGAACCGTCGTT
TGAAAGATAAGGAAGAGGTGGAGGCATTCCTTGAATCTTGCAAGGACGCTTCTTTCACGATCGATGATATCTCGAAAAAG
CCGGTGCGTAAAACACCAGCCCCTCCTTTTACGACATCTACCTTGCAGCAGGAGGCCGCCCGTAAGTTGGGTTATTCGGT
CTCGCAGACCATGATGATCGCCCAACGCCTGTATGAGTCCGGATTGATCACGTATATGCGTACTGACTCCGTGAACTTAA
GTGATTTGGCTTTGGGGACGGCAAAAGAGGCTATCTTGGATACCTACGGTGAGAAATATTATAAATTCCGTCAATATCAT
ACCAAGAGCAAGGGAGCGCAAGAAGCGCACGAGGCGATTCGTCCGACCTATATCAGCAATGATGAGATAAGCGGGACCGC
TCAGGAAAAGAGATTATACGAATTGATCCGCAAACGTACGATCGCTTGCCAGATGGCGGACGCCGAGTTGGAGCGTACGA
CGATCTCCGTAGGCATCGGCGGCAAGAAAGAGAAGTTCGTGGCTACCGGTGAGGTCATTACTTTTGACGGTTTCCTTCAG
GTGTACCGCGAGAGTTTTGATGACGAGAATGAGAAGGAACAGGAAAACGGCCTTCTTCCTCCGGTTACCTTGAACGAGGT
ATTGAGTATGAAGGATATCGTCGCTACCGAACGTTTCACGCAACGTCCTCCCCGCTATACGGAAGCAAGTTTGGTTCGCC
GTTTGGAGGAATTGGGAATCGGTCGTCCTTCTACCTATGCTCCTACGATCCAGACGATCCAGAATCGAGAGTATGTTGTG
AAAGGGGATAAGGAAGGAACGGAGCGTACTTATAATATCATAACCTTGGACCGGCATACGATCAAGGAAGTCCAAAAGAC
GGAGATCGTAGGAGCGGACCGGAATAAGTTGATGCCAACCGATATCGGTACGGTCGTGAATGATTTCTTGATGGAGTATT
TCCCGGGCGTTATGGATTATAACTTCACGGCGAGCGTAGAGAAGGAATTCGATGCCGTAGCGGAAGGCGAGATGGTATGG
ACGGACGCTATCGATAAATTCTATAAACTATTCCATCCGATCGTGGAAGAGGCAGCCTCTGTCCGTACGGAGCATAAAGT
GGGTGAGCGTGAATTGGGTATCGATCCGAAGAGCGGCAAACCTGTTTTCGTGAAGATCGGTCGTTATGGCCCAGTCGTAC
AGATCGGGCAGGCGCACCCAGAGGATAAAGAGGCTCCTAAACCTCAGTTCGCAACCTTGATGAAGGGGCAATCTATCGAG
ACGATTACTTTGGAAGAAGCCTTGAAATTGTTTGATCTACCCCGTACGATTGGTGAATATGAAGGTAAGGTCATGGTTGC
CGCTGTCGGACGTTTTGGCCCGTTTATCCGTCATGACGGAAAGTTCGTATCCATACCGAAGGGCTTGAATCCTTTGTCGA
TAACAGCCGAGGAAGCTATCGAGTTGATCGAGGCAAAAAAACAAAAAGATGAGCAACGATTTATCAAGAAGTTCGATGAG
GATCCGGAAATGGAGATCTTGAATGGTCGCTTCGGCCCTTATATCTCTTATAAGAAAAAGAATTACAGAATCCCTAAGAC
GATAGAGAAACCAGAGAACTTGACCTTGGAGGAATGCCAGAAGATAATCGCCGAGGCGGGAGAAAAACCTGCTGCGACGA
AGAAACGTACGACACGGAAAAAGGCTTGA

Upstream 100 bases:

>100_bases
ATAATGTATAGGAAAAAACGCTTTTTTAGTGACCGATATACTGCGTACGTTAATTATTTGTGTAAGTTTGCACCCGATTT
CTAACCAGCAAAAAATAGAT

Downstream 100 bases:

>100_bases
TTATAGCTCTAAAAATTCATAGTTATGATTTACTAAACTCATAACTATGAATTTTTCAAAACATAACTATGATTTTTTAG
ACATCGATGCCCGTAAATTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MQKNLVIVESPAKAKTIEKFLGKDYKVMSSYGHIRDLKTKDFSIDLEHNYAPEYVIPSDKKKLVTELKNEAKKAEQVWLA
SDEDREGEAISWHLYEVLGLKPENTKRIVFHEITKNAILHAIETPRDINIDLVNAQQARRILDRIVGFELSPILWRKVKP
ALSAGRVQSVAVRLIVEREREINEFVSEAAYRVIASFILPDGTTILKAELNRRLKDKEEVEAFLESCKDASFTIDDISKK
PVRKTPAPPFTTSTLQQEAARKLGYSVSQTMMIAQRLYESGLITYMRTDSVNLSDLALGTAKEAILDTYGEKYYKFRQYH
TKSKGAQEAHEAIRPTYISNDEISGTAQEKRLYELIRKRTIACQMADAELERTTISVGIGGKKEKFVATGEVITFDGFLQ
VYRESFDDENEKEQENGLLPPVTLNEVLSMKDIVATERFTQRPPRYTEASLVRRLEELGIGRPSTYAPTIQTIQNREYVV
KGDKEGTERTYNIITLDRHTIKEVQKTEIVGADRNKLMPTDIGTVVNDFLMEYFPGVMDYNFTASVEKEFDAVAEGEMVW
TDAIDKFYKLFHPIVEEAASVRTEHKVGERELGIDPKSGKPVFVKIGRYGPVVQIGQAHPEDKEAPKPQFATLMKGQSIE
TITLEEALKLFDLPRTIGEYEGKVMVAAVGRFGPFIRHDGKFVSIPKGLNPLSITAEEAIELIEAKKQKDEQRFIKKFDE
DPEMEILNGRFGPYISYKKKNYRIPKTIEKPENLTLEECQKIIAEAGEKPAATKKRTTRKKA

Sequences:

>Translated_782_residues
MQKNLVIVESPAKAKTIEKFLGKDYKVMSSYGHIRDLKTKDFSIDLEHNYAPEYVIPSDKKKLVTELKNEAKKAEQVWLA
SDEDREGEAISWHLYEVLGLKPENTKRIVFHEITKNAILHAIETPRDINIDLVNAQQARRILDRIVGFELSPILWRKVKP
ALSAGRVQSVAVRLIVEREREINEFVSEAAYRVIASFILPDGTTILKAELNRRLKDKEEVEAFLESCKDASFTIDDISKK
PVRKTPAPPFTTSTLQQEAARKLGYSVSQTMMIAQRLYESGLITYMRTDSVNLSDLALGTAKEAILDTYGEKYYKFRQYH
TKSKGAQEAHEAIRPTYISNDEISGTAQEKRLYELIRKRTIACQMADAELERTTISVGIGGKKEKFVATGEVITFDGFLQ
VYRESFDDENEKEQENGLLPPVTLNEVLSMKDIVATERFTQRPPRYTEASLVRRLEELGIGRPSTYAPTIQTIQNREYVV
KGDKEGTERTYNIITLDRHTIKEVQKTEIVGADRNKLMPTDIGTVVNDFLMEYFPGVMDYNFTASVEKEFDAVAEGEMVW
TDAIDKFYKLFHPIVEEAASVRTEHKVGERELGIDPKSGKPVFVKIGRYGPVVQIGQAHPEDKEAPKPQFATLMKGQSIE
TITLEEALKLFDLPRTIGEYEGKVMVAAVGRFGPFIRHDGKFVSIPKGLNPLSITAEEAIELIEAKKQKDEQRFIKKFDE
DPEMEILNGRFGPYISYKKKNYRIPKTIEKPENLTLEECQKIIAEAGEKPAATKKRTTRKKA
>Mature_782_residues
MQKNLVIVESPAKAKTIEKFLGKDYKVMSSYGHIRDLKTKDFSIDLEHNYAPEYVIPSDKKKLVTELKNEAKKAEQVWLA
SDEDREGEAISWHLYEVLGLKPENTKRIVFHEITKNAILHAIETPRDINIDLVNAQQARRILDRIVGFELSPILWRKVKP
ALSAGRVQSVAVRLIVEREREINEFVSEAAYRVIASFILPDGTTILKAELNRRLKDKEEVEAFLESCKDASFTIDDISKK
PVRKTPAPPFTTSTLQQEAARKLGYSVSQTMMIAQRLYESGLITYMRTDSVNLSDLALGTAKEAILDTYGEKYYKFRQYH
TKSKGAQEAHEAIRPTYISNDEISGTAQEKRLYELIRKRTIACQMADAELERTTISVGIGGKKEKFVATGEVITFDGFLQ
VYRESFDDENEKEQENGLLPPVTLNEVLSMKDIVATERFTQRPPRYTEASLVRRLEELGIGRPSTYAPTIQTIQNREYVV
KGDKEGTERTYNIITLDRHTIKEVQKTEIVGADRNKLMPTDIGTVVNDFLMEYFPGVMDYNFTASVEKEFDAVAEGEMVW
TDAIDKFYKLFHPIVEEAASVRTEHKVGERELGIDPKSGKPVFVKIGRYGPVVQIGQAHPEDKEAPKPQFATLMKGQSIE
TITLEEALKLFDLPRTIGEYEGKVMVAAVGRFGPFIRHDGKFVSIPKGLNPLSITAEEAIELIEAKKQKDEQRFIKKFDE
DPEMEILNGRFGPYISYKKKNYRIPKTIEKPENLTLEECQKIIAEAGEKPAATKKRTTRKKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=457, Percent_Identity=28.0087527352298, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI1787529, Length=619, Percent_Identity=42.0032310177706, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1788061, Length=442, Percent_Identity=27.8280542986425, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=451, Percent_Identity=26.3858093126386, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=490, Percent_Identity=26.530612244898, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=498, Percent_Identity=24.6987951807229, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585251, Length=452, Percent_Identity=28.7610619469027, Blast_Score=137, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24640096, Length=533, Percent_Identity=23.4521575984991, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 88927; Mature: 88927

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNLVIVESPAKAKTIEKFLGKDYKVMSSYGHIRDLKTKDFSIDLEHNYAPEYVIPSDK
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCHHCCCCCCEEEEECCCCCCCEECCCHH
KKLVTELKNEAKKAEQVWLASDEDREGEAISWHLYEVLGLKPENTKRIVFHEITKNAILH
HHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEHHHHHHHHHH
AIETPRDINIDLVNAQQARRILDRIVGFELSPILWRKVKPALSAGRVQSVAVRLIVERER
HHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINEFVSEAAYRVIASFILPDGTTILKAELNRRLKDKEEVEAFLESCKDASFTIDDISKK
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHCCCC
PVRKTPAPPFTTSTLQQEAARKLGYSVSQTMMIAQRLYESGLITYMRTDSVNLSDLALGT
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
AKEAILDTYGEKYYKFRQYHTKSKGAQEAHEAIRPTYISNDEISGTAQEKRLYELIRKRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
IACQMADAELERTTISVGIGGKKEKFVATGEVITFDGFLQVYRESFDDENEKEQENGLLP
HHHHHHCCHHEEEEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCHHHHHCCCCC
PVTLNEVLSMKDIVATERFTQRPPRYTEASLVRRLEELGIGRPSTYAPTIQTIQNREYVV
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
KGDKEGTERTYNIITLDRHTIKEVQKTEIVGADRNKLMPTDIGTVVNDFLMEYFPGVMDY
ECCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
NFTASVEKEFDAVAEGEMVWTDAIDKFYKLFHPIVEEAASVRTEHKVGERELGIDPKSGK
CCEECHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCC
PVFVKIGRYGPVVQIGQAHPEDKEAPKPQFATLMKGQSIETITLEEALKLFDLPRTIGEY
EEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHCHHHHHHC
EGKVMVAAVGRFGPFIRHDGKFVSIPKGLNPLSITAEEAIELIEAKKQKDEQRFIKKFDE
CCCEEEEEHHHHCCHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCC
DPEMEILNGRFGPYISYKKKNYRIPKTIEKPENLTLEECQKIIAEAGEKPAATKKRTTRK
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
KA
CC
>Mature Secondary Structure
MQKNLVIVESPAKAKTIEKFLGKDYKVMSSYGHIRDLKTKDFSIDLEHNYAPEYVIPSDK
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCHHCCCCCCEEEEECCCCCCCEECCCHH
KKLVTELKNEAKKAEQVWLASDEDREGEAISWHLYEVLGLKPENTKRIVFHEITKNAILH
HHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEHHHHHHHHHH
AIETPRDINIDLVNAQQARRILDRIVGFELSPILWRKVKPALSAGRVQSVAVRLIVERER
HHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINEFVSEAAYRVIASFILPDGTTILKAELNRRLKDKEEVEAFLESCKDASFTIDDISKK
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHCCCC
PVRKTPAPPFTTSTLQQEAARKLGYSVSQTMMIAQRLYESGLITYMRTDSVNLSDLALGT
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
AKEAILDTYGEKYYKFRQYHTKSKGAQEAHEAIRPTYISNDEISGTAQEKRLYELIRKRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
IACQMADAELERTTISVGIGGKKEKFVATGEVITFDGFLQVYRESFDDENEKEQENGLLP
HHHHHHCCHHEEEEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCHHHHHCCCCC
PVTLNEVLSMKDIVATERFTQRPPRYTEASLVRRLEELGIGRPSTYAPTIQTIQNREYVV
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
KGDKEGTERTYNIITLDRHTIKEVQKTEIVGADRNKLMPTDIGTVVNDFLMEYFPGVMDY
ECCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
NFTASVEKEFDAVAEGEMVWTDAIDKFYKLFHPIVEEAASVRTEHKVGERELGIDPKSGK
CCEECHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCC
PVFVKIGRYGPVVQIGQAHPEDKEAPKPQFATLMKGQSIETITLEEALKLFDLPRTIGEY
EEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHCHHHHHHC
EGKVMVAAVGRFGPFIRHDGKFVSIPKGLNPLSITAEEAIELIEAKKQKDEQRFIKKFDE
CCCEEEEEHHHHCCHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCC
DPEMEILNGRFGPYISYKKKNYRIPKTIEKPENLTLEECQKIIAEAGEKPAATKKRTTRK
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]