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The map label for this gene is gloA [C]

Identifier: 150010165

GI number: 150010165

Start: 4396266

End: 4396643

Strand: Direct

Name: gloA [C]

Synonym: BDI_3588

Alternate gene names: 150010165

Gene position: 4396266-4396643 (Clockwise)

Preceding gene: 150010164

Following gene: 150010166

Centisome position: 91.37

GC content: 41.8

Gene sequence:

>378_bases
ATGGAAGTTAAAGGTAGATTTGACCATTTCAATATTAATGTATTGGATTTGGATAAGAGTATCGCTTTTTATAATAAAGC
GTTGGGATTGAAAGAGCACCATCGTAAGGTAGCGGAAGACGGTTCGTTTATCTTGGTTTATCTAACGGATGAGCAAACCG
GTTTCTTGATGGAGCTGACTTGGCTGCGTGACCGGAAAGAACCTTATGAGCTAGGCGACAATGAGAGCCATCTTTGTTTC
CGGGTAGCCGGCGATTATGAGGAGGTACGTAAATACCACAAGGAAATGGGAGCGGTTTGCTTCGAGAATACCAAGATGGG
ATTGTATTTTATTAATGATCCGGATGACTATTGGATCGAGATATTACCGTTGCGATAA

Upstream 100 bases:

>100_bases
AAGGATGTCGTTATATCGTATTGTCTGTTCCGGGAGAAGGGACGGATATATAATATATTATTAATGTATAGGTTATTTTA
AACGCATAAATTTATTAGGT

Downstream 100 bases:

>100_bases
TTCTCCATTATTATATAACAATGTTGCTTAACATAAGTTTTCGAAAAAAGAAGATAAAATGACAGGAATCTGTAAAATTT
TCTTCAAATCGTATTGCAAC

Product: lactoylglutathione lyase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: Glyoxalase/Bleomycin Resistance Protein/Dioxygenase; Lactoylglutathione Lyase-Like Lyase; Glyoxalase Family Protein; Lactoylglutathione Lyase Protein; Glyoxalase I

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MEVKGRFDHFNINVLDLDKSIAFYNKALGLKEHHRKVAEDGSFILVYLTDEQTGFLMELTWLRDRKEPYELGDNESHLCF
RVAGDYEEVRKYHKEMGAVCFENTKMGLYFINDPDDYWIEILPLR

Sequences:

>Translated_125_residues
MEVKGRFDHFNINVLDLDKSIAFYNKALGLKEHHRKVAEDGSFILVYLTDEQTGFLMELTWLRDRKEPYELGDNESHLCF
RVAGDYEEVRKYHKEMGAVCFENTKMGLYFINDPDDYWIEILPLR
>Mature_125_residues
MEVKGRFDHFNINVLDLDKSIAFYNKALGLKEHHRKVAEDGSFILVYLTDEQTGFLMELTWLRDRKEPYELGDNESHLCF
RVAGDYEEVRKYHKEMGAVCFENTKMGLYFINDPDDYWIEILPLR

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 14857; Mature: 14857

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKGRFDHFNINVLDLDKSIAFYNKALGLKEHHRKVAEDGSFILVYLTDEQTGFLMELT
CCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEE
WLRDRKEPYELGDNESHLCFRVAGDYEEVRKYHKEMGAVCFENTKMGLYFINDPDDYWIE
ECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCEEEE
ILPLR
EEECC
>Mature Secondary Structure
MEVKGRFDHFNINVLDLDKSIAFYNKALGLKEHHRKVAEDGSFILVYLTDEQTGFLMELT
CCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEE
WLRDRKEPYELGDNESHLCFRVAGDYEEVRKYHKEMGAVCFENTKMGLYFINDPDDYWIE
ECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCEEEE
ILPLR
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA