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The map label for this gene is purT

Identifier: 150010155

GI number: 150010155

Start: 4375959

End: 4377140

Strand: Direct

Name: purT

Synonym: BDI_3578

Alternate gene names: 150010155

Gene position: 4375959-4377140 (Clockwise)

Preceding gene: 150010145

Following gene: 150010162

Centisome position: 90.95

GC content: 53.64

Gene sequence:

>1182_bases
ATGGTAACAATTGGTACGCCCAAATCGGCTACGGCAACTAAAGTCGTGCTTTGTGGCTCGGGCGAATTGGGGAAAGAGTT
TGTAATAGAGTTGCAGCGTTATGGCGTGGAGGTAATCGCCTTGGATAAGTATCCGGACGCTCCGGCCATGCAGGTGGCGC
ATCGTTCCTATGTGGTCTCCATGCTGGATGGCGATGCCTTGCGGGAGATCATCGAGAAAGAGAAACCGGATTATATCGTG
CCGGAGGTGGAAGCGATCGCTACCCGTACCTTGATGGAACTGGAGGAAGAGGGATACCATGTGATTCCTACCGCCCGTGC
CACTTGGCTGACGATGAACCGTGAGGGAATCCGCCGCTTGGCGGCCGAGGAACTGGGCTTGCCTACCTCGCCGTATCGTT
TCGCCGAGACCGAGGAGGAATTTAAAGAAGCGGTGAAGGTAATCGGTATGCCTTGCGTGGTGAAGCCGATCATGAGCTCG
AGCGGACACGGACAGAGCGTGGTTCGTGAGGAAAAGGATATCGATCGTGCTTGGCAGTACGCCCAAGAAGGAGGACGTGC
TGGAGCGGGTAAGGTGATCGTGGAAGGTTTCGTGGATTTCGACTATGAGATTACCCAGCTGACGGTTCGGCATATCGGAG
GAACGTCTTTCTTGGAACCCGTAGGACATGTTCAGGTGGACGGTGATTACCGGGAGTCTTGGCAACCGCAAGCCATGAGC
CTTGCCGCTAAAGCGAAAGCCCGTGAGATCGCCGGTAAGATCACCGAGGCTTTAGGCGGACGTGGTATTTTTGGCGTGGA
ATTATTCATTAAAGGAGATGATGTCATATTCAGCGAGGTTTCCCCCCGTCCGCACGATACCGGAATGGTGACGATGATTA
CGCAAGATTTGTCTCAATTCGCTTTGCATGCCCGTGCGGTGCTTGGCTTGCCGATCCCTAATATCGTGTTTCATGGAGCG
GGAGCTTCCCGTGCCGTTGTAGTGGAAGGGGACAGTGACCGGTTGTCTTTGGGGAATCTGGATAAGGTTTTGGAACAACC
CGATACGCAGATGCGTTTCTTTGGAAAACCGGAAGTTCATGGACATCGCCGTATGGCCGTCTTGCTGGCTCGTGGTTTGG
ATGTGACGGAGGCAAGGGAGAAGACCGGAGTGATGATGGAGCAGTTAAATGTTAAATTGTGA

Upstream 100 bases:

>100_bases
GCTAACCTTATCTTTGCCCTAATAGATAAAGGGAAAAGCTTTAGGCTATACAGGCATAAACCCTTAACTTTGCGGCGAAA
TTCAAATAGCAGAAAGATTT

Downstream 100 bases:

>100_bases
TGCTTCCTAGGATAAATGAACGTTTTGGTGTGTGTTGTGAATAAAATGTGAATATTTACAGACCTATTTTTCTATCAAAA
TAGTTTATTTTTGTCTCAAG

Product: phosphoribosylglycinamide formyltransferase

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVTIGTPKSATATKVVLCGSGELGKEFVIELQRYGVEVIALDKYPDAPAMQVAHRSYVVSMLDGDALREIIEKEKPDYIV
PEVEAIATRTLMELEEEGYHVIPTARATWLTMNREGIRRLAAEELGLPTSPYRFAETEEEFKEAVKVIGMPCVVKPIMSS
SGHGQSVVREEKDIDRAWQYAQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMS
LAAKAKAREIAGKITEALGGRGIFGVELFIKGDDVIFSEVSPRPHDTGMVTMITQDLSQFALHARAVLGLPIPNIVFHGA
GASRAVVVEGDSDRLSLGNLDKVLEQPDTQMRFFGKPEVHGHRRMAVLLARGLDVTEAREKTGVMMEQLNVKL

Sequences:

>Translated_393_residues
MVTIGTPKSATATKVVLCGSGELGKEFVIELQRYGVEVIALDKYPDAPAMQVAHRSYVVSMLDGDALREIIEKEKPDYIV
PEVEAIATRTLMELEEEGYHVIPTARATWLTMNREGIRRLAAEELGLPTSPYRFAETEEEFKEAVKVIGMPCVVKPIMSS
SGHGQSVVREEKDIDRAWQYAQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMS
LAAKAKAREIAGKITEALGGRGIFGVELFIKGDDVIFSEVSPRPHDTGMVTMITQDLSQFALHARAVLGLPIPNIVFHGA
GASRAVVVEGDSDRLSLGNLDKVLEQPDTQMRFFGKPEVHGHRRMAVLLARGLDVTEAREKTGVMMEQLNVKL
>Mature_393_residues
MVTIGTPKSATATKVVLCGSGELGKEFVIELQRYGVEVIALDKYPDAPAMQVAHRSYVVSMLDGDALREIIEKEKPDYIV
PEVEAIATRTLMELEEEGYHVIPTARATWLTMNREGIRRLAAEELGLPTSPYRFAETEEEFKEAVKVIGMPCVVKPIMSS
SGHGQSVVREEKDIDRAWQYAQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMS
LAAKAKAREIAGKITEALGGRGIFGVELFIKGDDVIFSEVSPRPHDTGMVTMITQDLSQFALHARAVLGLPIPNIVFHGA
GASRAVVVEGDSDRLSLGNLDKVLEQPDTQMRFFGKPEVHGHRRMAVLLARGLDVTEAREKTGVMMEQLNVKL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=60.7142857142857, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786733, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=353, Percent_Identity=23.7960339943343, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PARD8 (A6LHW2)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304898.1
- ProteinModelPortal:   A6LHW2
- SMR:   A6LHW2
- STRING:   A6LHW2
- GeneID:   5308727
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3578
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- BioCyc:   PDIS435591:BDI_3578-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43172; Mature: 43172

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIGTPKSATATKVVLCGSGELGKEFVIELQRYGVEVIALDKYPDAPAMQVAHRSYVVS
CEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDGDALREIIEKEKPDYIVPEVEAIATRTLMELEEEGYHVIPTARATWLTMNREGIRRL
HHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECHHHHHHH
AAEELGLPTSPYRFAETEEEFKEAVKVIGMPCVVKPIMSSSGHGQSVVREEKDIDRAWQY
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMS
HHHCCCCCCCCEEEEEECCCCEEEHEEEHHHCCCCHHHCCCCCEEECCCHHCCCCCHHHH
LAAKAKAREIAGKITEALGGRGIFGVELFIKGDDVIFSEVSPRPHDTGMVTMITQDLSQF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEHHHHHHHHH
ALHARAVLGLPIPNIVFHGAGASRAVVVEGDSDRLSLGNLDKVLEQPDTQMRFFGKPEVH
HHHHHHHHCCCCCHHEEECCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCC
GHRRMAVLLARGLDVTEAREKTGVMMEQLNVKL
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTIGTPKSATATKVVLCGSGELGKEFVIELQRYGVEVIALDKYPDAPAMQVAHRSYVVS
CEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDGDALREIIEKEKPDYIVPEVEAIATRTLMELEEEGYHVIPTARATWLTMNREGIRRL
HHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECHHHHHHH
AAEELGLPTSPYRFAETEEEFKEAVKVIGMPCVVKPIMSSSGHGQSVVREEKDIDRAWQY
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMS
HHHCCCCCCCCEEEEEECCCCEEEHEEEHHHCCCCHHHCCCCCEEECCCHHCCCCCHHHH
LAAKAKAREIAGKITEALGGRGIFGVELFIKGDDVIFSEVSPRPHDTGMVTMITQDLSQF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEHHHHHHHHH
ALHARAVLGLPIPNIVFHGAGASRAVVVEGDSDRLSLGNLDKVLEQPDTQMRFFGKPEVH
HHHHHHHHCCCCCHHEEECCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCC
GHRRMAVLLARGLDVTEAREKTGVMMEQLNVKL
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA