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The map label for this gene is nusA [H]

Identifier: 150010113

GI number: 150010113

Start: 4323018

End: 4324283

Strand: Direct

Name: nusA [H]

Synonym: BDI_3533

Alternate gene names: 150010113

Gene position: 4323018-4324283 (Clockwise)

Preceding gene: 150010112

Following gene: 150010114

Centisome position: 89.85

GC content: 47.0

Gene sequence:

>1266_bases
ATGGCCAAGAAAGAGGAAACAATCAGTATGATTGACACCCTTGCGGAGTTCAAGGAGTTGAAGAATATCGATAAAGATAC
GATGATTAGCGTTTTGGAAGATTCGTTCCGTAACGTGATCGCCAAGATGTTTGGCACGGATGAGAACTATGACGTGATTA
TCAACCCTGAGAAAGGTGATTTCGAGATATGGAGAAACCGTACGGTTGTGGCTGATGATGAATTGGAGGATGAGAACTTG
CAGCTTACGTTGACGGAGGCCCGTAAAATCGATGCCGACTGTGAGGTGGGCGAGGAAGTGACCGACGAGGTGCATTTCGC
AGACTTCGGACGCCGTGCGATCTTAAACTTGCGCCAAACTTTGGCTTCAAAGATACTTGAATTGCAGAAAGACAGCTTGT
ACGCTAAGTATAAGGATAAAATCGGCCACATCATCGCCGCTGAGGTTTATCAGGTGTGGAAGAAAGAAATCCTGTTGCTG
GATGACGATGGTAATGAGTTGCTACTGCCGAAATCCGAGCAAATCCCGACCGATTTCTATCGTAAGGGAGAGACCGTACG
TGCGATCGTTCAACGTGTGGATAACTATAATAACAACCCGAAGATCCTTCTTTCCCGTACGGATAAGGTCTTCTTGCAAC
GGTTGTTTGAGCTGGAGGTTCCGGAGATTAATGACGGCTTGATTACGATCAAGGCGATCGCCCGCATTCCGGGAGAGCGT
GCCAAGGTGGCTGTCGAGTCATACGATGACCGTATTGATCCGGTAGGCGCTTGCGTAGGTATGAAAGGCTCTCGTATCCA
CGGAATCGTTCGCGAGTTGAGAAACGAGAACATTGATGTGATTAATTACACCTCAAATCTCTCTTTGTTTATCCAGCGTG
CCTTAAGCCCGGCTAAGATCTCTTCTATCCGTGTGAATGAGGAGGAAAAGAAAGCTGAGGTTTATCTACGTCCGGAGGAG
GTTTCTTTAGCGATCGGTAAGGGCGGTCTGAATATTAAGTTGGCTTGTATGTTGACTGAGTATACGATTGACGTATTCCG
TGATATCGAAGGAGCGGACGAGGAGGATATCTACTTGGATGAGTTCTCCGACGAGATCGACAGCTGGGTGATCGACGCAC
TGAAGAATATAGGATGCTATACGGCGAAGAGTGTCCTTGCTATGGACCGCAATGAGATTATTGAACGTGCCGATCTGGAA
GAACAGACCGTGGACGATCTTTTAGCTATCTTATCTGCGGAATTTGAAGATGAAGGTAACGAATAA

Upstream 100 bases:

>100_bases
CCGGAAGGCGCTAAGCGGAAGGTGACTGTGGTGGAGGATGAGTCGTATACATTTGATGAAATAAAATATACAAAATACTT
AATAAGATTCAAGTAAGATT

Downstream 100 bases:

>100_bases
GTATTACGACAAAGTTAGATATGCCTATAAAATTAATTCAAGTACAGAGAAAATTGAACGTGGGAATCAACACGGTGGTT
GAGTTCCTGCACCGGAATGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAKKEETISMIDTLAEFKELKNIDKDTMISVLEDSFRNVIAKMFGTDENYDVIINPEKGDFEIWRNRTVVADDELEDENL
QLTLTEARKIDADCEVGEEVTDEVHFADFGRRAILNLRQTLASKILELQKDSLYAKYKDKIGHIIAAEVYQVWKKEILLL
DDDGNELLLPKSEQIPTDFYRKGETVRAIVQRVDNYNNNPKILLSRTDKVFLQRLFELEVPEINDGLITIKAIARIPGER
AKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDVINYTSNLSLFIQRALSPAKISSIRVNEEEKKAEVYLRPEE
VSLAIGKGGLNIKLACMLTEYTIDVFRDIEGADEEDIYLDEFSDEIDSWVIDALKNIGCYTAKSVLAMDRNEIIERADLE
EQTVDDLLAILSAEFEDEGNE

Sequences:

>Translated_421_residues
MAKKEETISMIDTLAEFKELKNIDKDTMISVLEDSFRNVIAKMFGTDENYDVIINPEKGDFEIWRNRTVVADDELEDENL
QLTLTEARKIDADCEVGEEVTDEVHFADFGRRAILNLRQTLASKILELQKDSLYAKYKDKIGHIIAAEVYQVWKKEILLL
DDDGNELLLPKSEQIPTDFYRKGETVRAIVQRVDNYNNNPKILLSRTDKVFLQRLFELEVPEINDGLITIKAIARIPGER
AKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDVINYTSNLSLFIQRALSPAKISSIRVNEEEKKAEVYLRPEE
VSLAIGKGGLNIKLACMLTEYTIDVFRDIEGADEEDIYLDEFSDEIDSWVIDALKNIGCYTAKSVLAMDRNEIIERADLE
EQTVDDLLAILSAEFEDEGNE
>Mature_420_residues
AKKEETISMIDTLAEFKELKNIDKDTMISVLEDSFRNVIAKMFGTDENYDVIINPEKGDFEIWRNRTVVADDELEDENLQ
LTLTEARKIDADCEVGEEVTDEVHFADFGRRAILNLRQTLASKILELQKDSLYAKYKDKIGHIIAAEVYQVWKKEILLLD
DDGNELLLPKSEQIPTDFYRKGETVRAIVQRVDNYNNNPKILLSRTDKVFLQRLFELEVPEINDGLITIKAIARIPGERA
KVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDVINYTSNLSLFIQRALSPAKISSIRVNEEEKKAEVYLRPEEV
SLAIGKGGLNIKLACMLTEYTIDVFRDIEGADEEDIYLDEFSDEIDSWVIDALKNIGCYTAKSVLAMDRNEIIERADLEE
QTVDDLLAILSAEFEDEGNE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=393, Percent_Identity=29.2620865139949, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 47983; Mature: 47852

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKEETISMIDTLAEFKELKNIDKDTMISVLEDSFRNVIAKMFGTDENYDVIINPEKGD
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
FEIWRNRTVVADDELEDENLQLTLTEARKIDADCEVGEEVTDEVHFADFGRRAILNLRQT
HHHHCCCEEEECCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASKILELQKDSLYAKYKDKIGHIIAAEVYQVWKKEILLLDDDGNELLLPKSEQIPTDFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCCCHHHH
RKGETVRAIVQRVDNYNNNPKILLSRTDKVFLQRLFELEVPEINDGLITIKAIARIPGER
HCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCH
AKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDVINYTSNLSLFIQRALSPAKI
HEEEHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHH
SSIRVNEEEKKAEVYLRPEEVSLAIGKGGLNIKLACMLTEYTIDVFRDIEGADEEDIYLD
CEEECCCHHCCCEEEECCCCEEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEEHH
EFSDEIDSWVIDALKNIGCYTAKSVLAMDRNEIIERADLEEQTVDDLLAILSAEFEDEGN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
E
C
>Mature Secondary Structure 
AKKEETISMIDTLAEFKELKNIDKDTMISVLEDSFRNVIAKMFGTDENYDVIINPEKGD
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
FEIWRNRTVVADDELEDENLQLTLTEARKIDADCEVGEEVTDEVHFADFGRRAILNLRQT
HHHHCCCEEEECCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASKILELQKDSLYAKYKDKIGHIIAAEVYQVWKKEILLLDDDGNELLLPKSEQIPTDFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCCCHHHH
RKGETVRAIVQRVDNYNNNPKILLSRTDKVFLQRLFELEVPEINDGLITIKAIARIPGER
HCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCH
AKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDVINYTSNLSLFIQRALSPAKI
HEEEHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHH
SSIRVNEEEKKAEVYLRPEEVSLAIGKGGLNIKLACMLTEYTIDVFRDIEGADEEDIYLD
CEEECCCHHCCCEEEECCCCEEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEEHH
EFSDEIDSWVIDALKNIGCYTAKSVLAMDRNEIIERADLEEQTVDDLLAILSAEFEDEGN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]