Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 150009602

GI number: 150009602

Start: 3610436

End: 3610966

Strand: Direct

Name: rlmB [H]

Synonym: BDI_3015

Alternate gene names: 150009602

Gene position: 3610436-3610966 (Clockwise)

Preceding gene: 150009599

Following gene: 150009604

Centisome position: 75.04

GC content: 42.75

Gene sequence:

>531_bases
ATGCGAAAATTAAAAGTAACCGAATTGAACCGCCTGACTCCCGAGGCTTTTAAGGAAAGTAAGAAGATACCTTTAATCGT
TGTCTTAGACCATATTAGAAGTCTTAACAACGTAGGTTCCGTGTTTCGTACCTCCGATGCTTTCCGGGTAGAGGCTATTT
ATCTTTGTGGTATTACGGCTTGCCCTCCGAACGCGGAGATCCATAAGACCGCCTTAGGAGCGGAAGAAACCGTAGATTGG
GAATATTTCAAGGATACCCCGGAGGCTGTTGATAAACTGCGCCAAGAGGGATATACGGTATGTGCCGTAGAGCAAGCGGA
AGGTAGTGTCATGTTAGATAACTTGCAGTTAGACAAGACAAAGAAATACGCTATCGTCATGGGTAACGAAGTAAAAGGCG
TTCAACAAAATGTGGTTGATAACTGTGATATATGTATTGAAATACCTCAATACGGTACAAAGCATTCCTTGAACGTATCG
GTTACTACCGGTATCGTGATCTGGGATTTCTTCAAGCAACTATCAGATTAA

Upstream 100 bases:

>100_bases
AAGCAAGAAATATTTTTCATTTTATGTCCACTACTTATTAATAGCTTTTCAACGGCATATCCACGGATATTCGCTACTTT
CGCGGTCAAAGATATTTGTT

Downstream 100 bases:

>100_bases
ACCATTCTCAACCAATACGACGACCCGTTCGGTCATGGCATCTTTACCTTTCGGGTCGTATTCCGATTTCAGACTGGCTC
CGAACATTCCGGCGAAGAAA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MRKLKVTELNRLTPEAFKESKKIPLIVVLDHIRSLNNVGSVFRTSDAFRVEAIYLCGITACPPNAEIHKTALGAEETVDW
EYFKDTPEAVDKLRQEGYTVCAVEQAEGSVMLDNLQLDKTKKYAIVMGNEVKGVQQNVVDNCDICIEIPQYGTKHSLNVS
VTTGIVIWDFFKQLSD

Sequences:

>Translated_176_residues
MRKLKVTELNRLTPEAFKESKKIPLIVVLDHIRSLNNVGSVFRTSDAFRVEAIYLCGITACPPNAEIHKTALGAEETVDW
EYFKDTPEAVDKLRQEGYTVCAVEQAEGSVMLDNLQLDKTKKYAIVMGNEVKGVQQNVVDNCDICIEIPQYGTKHSLNVS
VTTGIVIWDFFKQLSD
>Mature_176_residues
MRKLKVTELNRLTPEAFKESKKIPLIVVLDHIRSLNNVGSVFRTSDAFRVEAIYLCGITACPPNAEIHKTALGAEETVDW
EYFKDTPEAVDKLRQEGYTVCAVEQAEGSVMLDNLQLDKTKKYAIVMGNEVKGVQQNVVDNCDICIEIPQYGTKHSLNVS
VTTGIVIWDFFKQLSD

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=152, Percent_Identity=33.5526315789474, Blast_Score=84, Evalue=6e-17,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=30.718954248366, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=26.9736842105263, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204953, Length=154, Percent_Identity=35.0649350649351, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355819, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19753; Mature: 19753

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLKVTELNRLTPEAFKESKKIPLIVVLDHIRSLNNVGSVFRTSDAFRVEAIYLCGITA
CCCCCCCHHHCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEC
CPPNAEIHKTALGAEETVDWEYFKDTPEAVDKLRQEGYTVCAVEQAEGSVMLDNLQLDKT
CCCCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCEEEECEEECCC
KKYAIVMGNEVKGVQQNVVDNCDICIEIPQYGTKHSLNVSVTTGIVIWDFFKQLSD
CEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLKVTELNRLTPEAFKESKKIPLIVVLDHIRSLNNVGSVFRTSDAFRVEAIYLCGITA
CCCCCCCHHHCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEC
CPPNAEIHKTALGAEETVDWEYFKDTPEAVDKLRQEGYTVCAVEQAEGSVMLDNLQLDKT
CCCCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCEEEECEEECCC
KKYAIVMGNEVKGVQQNVVDNCDICIEIPQYGTKHSLNVSVTTGIVIWDFFKQLSD
CEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]