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The map label for this gene is tolB [C]

Identifier: 150009575

GI number: 150009575

Start: 3577892

End: 3578806

Strand: Direct

Name: tolB [C]

Synonym: BDI_2988

Alternate gene names: 150009575

Gene position: 3577892-3578806 (Clockwise)

Preceding gene: 150009574

Following gene: 150009577

Centisome position: 74.36

GC content: 48.31

Gene sequence:

>915_bases
ATGAAACGTTTTCTCAATTCTGTACTTTGTATGTGTATCGCCGTTTTTTGTACGCATGCCCAAAAAAAGAATGTGACTAG
TGTGCTGGAAGTGATGGACATTACGACCGGGCAACGTTCCGTTGTCAAGGAATTCCCTTTTTTGATCGAGGCACCTAACT
GGACTCCGGATGGTAATTGGCTTGTATATAATAGTGGAGGAAAATTATATAAGCTCTCTCCGGAATCTCCGGGAGAGCCG
CAATTAATCAATAGCGACTACGCGACCCGTTGCAACAACGACCATGTGATCTCCGCGGATGGGAAGCAAATAGCGATCAG
TAACGGAACGAAAGAAGATGGCCGGTCGCGTATCTATACCCTGCCTTTCGAAGGAGGTGTTCCCCGATTGATTACCCCAC
TCGGTCCTAGCTACCTACACGGGTGGAGCCCCGACGGAAAACAACTCGCTTATTGCGCCGAGAGAAACGGTAATTTTGAT
GTTTACGTCATTCCTGCCCTAGGCGGAGAAGAAAAACGTCTTACGACAGCCGAAGGTTTGGACGATGGCCCCGAATACTC
ACCTTGCGGACAATATATCTGGTTCAACTCCGTACGTACCGGGCTTATGCAAGTCTGGCGCATGAAGGCGGATGGTTCCG
AACAAACCCAGATGACATTCGATGAGACCCGAAATTCTTGGTTTCCCCACATCTCTCCGGATGGAAAACAAATCGTTTTC
ATCACCTATACAAAAGGTGATCTGGAACCCGGACAACACCTCGCCAATAAAAACGTAGAGTTACGCCTTATGCCAGCCGA
GGGCGGAGAACCTAAGACTTTAGTCAAACTATTCGGCGGGCAAGGTACGATAAACGTAAACTCTTGGGCACCCGATAGCA
AGCGATTCGCTTTCGTCAGTTACCGGTTAAATTGA

Upstream 100 bases:

>100_bases
TATAAGGAGTAATCGAAATAAATTATATCTTTGAAACCGGGTAAAATAGCGTAAAGCTAATTTTACCCGGTTTTCTTATA
TCCTTTAAATCTATATGTAT

Downstream 100 bases:

>100_bases
CTCGTTCCCGTCTCTCCTGTATTCGGAAGGAATAACCCCTGTCTGCTTAATAAACGCACGACGGAACGTACTGATAGAAT
GGAAGCCCGAACGTTCGGCT

Product: Tol biopolymer transport system periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKRFLNSVLCMCIAVFCTHAQKKNVTSVLEVMDITTGQRSVVKEFPFLIEAPNWTPDGNWLVYNSGGKLYKLSPESPGEP
QLINSDYATRCNNDHVISADGKQIAISNGTKEDGRSRIYTLPFEGGVPRLITPLGPSYLHGWSPDGKQLAYCAERNGNFD
VYVIPALGGEEKRLTTAEGLDDGPEYSPCGQYIWFNSVRTGLMQVWRMKADGSEQTQMTFDETRNSWFPHISPDGKQIVF
ITYTKGDLEPGQHLANKNVELRLMPAEGGEPKTLVKLFGGQGTINVNSWAPDSKRFAFVSYRLN

Sequences:

>Translated_304_residues
MKRFLNSVLCMCIAVFCTHAQKKNVTSVLEVMDITTGQRSVVKEFPFLIEAPNWTPDGNWLVYNSGGKLYKLSPESPGEP
QLINSDYATRCNNDHVISADGKQIAISNGTKEDGRSRIYTLPFEGGVPRLITPLGPSYLHGWSPDGKQLAYCAERNGNFD
VYVIPALGGEEKRLTTAEGLDDGPEYSPCGQYIWFNSVRTGLMQVWRMKADGSEQTQMTFDETRNSWFPHISPDGKQIVF
ITYTKGDLEPGQHLANKNVELRLMPAEGGEPKTLVKLFGGQGTINVNSWAPDSKRFAFVSYRLN
>Mature_304_residues
MKRFLNSVLCMCIAVFCTHAQKKNVTSVLEVMDITTGQRSVVKEFPFLIEAPNWTPDGNWLVYNSGGKLYKLSPESPGEP
QLINSDYATRCNNDHVISADGKQIAISNGTKEDGRSRIYTLPFEGGVPRLITPLGPSYLHGWSPDGKQLAYCAERNGNFD
VYVIPALGGEEKRLTTAEGLDDGPEYSPCGQYIWFNSVRTGLMQVWRMKADGSEQTQMTFDETRNSWFPHISPDGKQIVF
ITYTKGDLEPGQHLANKNVELRLMPAEGGEPKTLVKLFGGQGTINVNSWAPDSKRFAFVSYRLN

Specific function: Involved In The Tonb-Independent Uptake Of Group A Colicins (Colicins A, E1, E2, E3 And K). Necessary For The Colicins To Reach Their Respective Targets After Initial Binding To The Bacteria. [C]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011042
- InterPro:   IPR011659 [H]

Pfam domain/function: PF07676 PD40 [H]

EC number: NA

Molecular weight: Translated: 33883; Mature: 33883

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFLNSVLCMCIAVFCTHAQKKNVTSVLEVMDITTGQRSVVKEFPFLIEAPNWTPDGNW
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCEEEECCCCCCCCCE
LVYNSGGKLYKLSPESPGEPQLINSDYATRCNNDHVISADGKQIAISNGTKEDGRSRIYT
EEECCCCEEEEECCCCCCCCCEECCCCHHCCCCCCEEECCCCEEEECCCCCCCCCCEEEE
LPFEGGVPRLITPLGPSYLHGWSPDGKQLAYCAERNGNFDVYVIPALGGEEKRLTTAEGL
ECCCCCCCEEECCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEEECCCC
DDGPEYSPCGQYIWFNSVRTGLMQVWRMKADGSEQTQMTFDETRNSWFPHISPDGKQIVF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEE
ITYTKGDLEPGQHLANKNVELRLMPAEGGEPKTLVKLFGGQGTINVNSWAPDSKRFAFVS
EEEECCCCCCCHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEE
YRLN
EEEC
>Mature Secondary Structure
MKRFLNSVLCMCIAVFCTHAQKKNVTSVLEVMDITTGQRSVVKEFPFLIEAPNWTPDGNW
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCEEEECCCCCCCCCE
LVYNSGGKLYKLSPESPGEPQLINSDYATRCNNDHVISADGKQIAISNGTKEDGRSRIYT
EEECCCCEEEEECCCCCCCCCEECCCCHHCCCCCCEEECCCCEEEECCCCCCCCCCEEEE
LPFEGGVPRLITPLGPSYLHGWSPDGKQLAYCAERNGNFDVYVIPALGGEEKRLTTAEGL
ECCCCCCCEEECCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEEECCCC
DDGPEYSPCGQYIWFNSVRTGLMQVWRMKADGSEQTQMTFDETRNSWFPHISPDGKQIVF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEE
ITYTKGDLEPGQHLANKNVELRLMPAEGGEPKTLVKLFGGQGTINVNSWAPDSKRFAFVS
EEEECCCCCCCHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEE
YRLN
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]