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The map label for this gene is ecaIM [H]

Identifier: 150009567

GI number: 150009567

Start: 3569415

End: 3571379

Strand: Reverse

Name: ecaIM [H]

Synonym: BDI_2980

Alternate gene names: 150009567

Gene position: 3571379-3569415 (Counterclockwise)

Preceding gene: 150009568

Following gene: 150009566

Centisome position: 74.23

GC content: 40.92

Gene sequence:

>1965_bases
ATGCCAGCAACATCCTTAATAGAAGAACTTCCTAAAATCGTAAAAGAAGGAAGACAAGAAGCGCAACGTATTTTAGAACG
TTTGAGTAGCAACACCCATATCGGATTACAAACAAATGAATTGGTTCTTCCCGCCAAAGATACTTCCGGATTATGGAAAG
GAAAAAACGAACAAGTGATAAACAAAGAATGGATGAACCGTCTCGTCTATGGCGATAACTTATTGGCGATGCAAGCCCTG
TTAGCCGGAGATGAAGCAACCGGATTACCCTCACTTCGGGGAAAGGTGGATTTGATTTATATCGATCCTCCTTTTGATAG
CAAAGCGGATTATCGGACAAAGATTAATTTGCCGGGTATGGATATTGAACAGAAGCCAACGGTAATCGAACAATTTGCTT
ATTCGGATACATGGCAAGATGGAACGGTTTCTTATTTGAAGATGCTTTATCCAAGATTAGTATTGATGCGGGAGTTACTT
TCGGATAAGGGAAGTATTTATGTACATATTGATTGGCATGTGGGAGCGTATGTGAAAGTTTTAATGGATGACGTGTTTGG
TAAAGATAATTTTGTAAATAACATAATATGGCACTATGGAGGTCCATCACCTATTAAAACAGCTTTTGCTAGAAAATATG
ATATTATTTATTTATATGGAAAAACATTAAAAAGGATTTTTAACCCACAATATGGAAATATACCCATATCCGTTTTAAAT
AGAGCAAAAAAAGACGAAAATGGGAGACTATGGCTTGATCAAAATCTGGGAAAATTAAAAGAAGATACTATTGCTAAAAT
GGAATCCGAAGGACGGTTATATAGAACTAAAACTGGAGGTCTAAGAAGAAAACAATATTTGGATGAAATGGAAGGAGCGC
AATTTGATAATGTATGGGATATTCCTATTATTAATTCTCAAGCAAAAGAAGACGTAGGGTATATAACCCAAAAACCCGAA
GCCCTCCTCGAACGCATCATCAAAGCCAGCAGCAACGAAGGTGATCTCGTTTGCGACTTCTTCGGTGGAAGTGGAACAAC
CGCTGCCGTTGCCGAACGGTTAGGCCGTCGTTGGATTACTACGGATATTGGCAAACCGGCAACCTTGGTGATGCGTAAAC
GCTTTATCGACCAAGAGGTAAAACCATTCCTTTATCAAGCCATCGGCGATTATCAGAAAGAGGCTTTCCAAAACAATAAA
CAATACAAACGGATTGGCGATTTGAGCCAGATCATCATGCAACTGTATGGCGCAATACCATTTACGCAGGAACAACTGAA
TGACCGGAACTGGGGATATATCAAGAACGGACGGACGTTGGTATTGGTAGATAGTCCCAATAAAGTAACCGGGGCAGCTA
CAATCCGCCGGGCATACGAAGCCAAAAACAATCTGTTGGGCGGTGGATGGAACAAGGCTGTCGTACTGGCATGGAACTTT
GCGTTTGATATTTCTGCTGCTATCCAACAATACAAGGAGGATGTTGAGGTCTTGGTGATTCCACCAGACCTGTTGGACAA
ACTCTCCAAGAAAGGATATGACAAACTGATAAGGGAAGGCTCTGTCCGTTTCAGCAGTTATCAATATCTATTGGTAAAAC
CTATTCAGACAGAACCGCATTTCGGTGAACAGGACAAACTAACAATCGAATTGGATAATTATGTGCTTCTTTCACCCGAC
AATATCCCGTTGGATGATAAAGATAAAGCCAAATTGCAACAAGTATTAGAGAAAGATCCATTGGCTTTGATCGAGTATTG
GAGCATCGATCCGGATTACGATGGCATCACATTCCGTAGCCAATGGCAAGACTATCGGGAAAATACGGACAATGATAGCG
ATCCGCTTCATTGCATCTATACCGCTACATTAATCACTCCTCGAAAAGCGGAACGGACGGTTTGCGTGAAAGCGGTCGAT
GTATTCGGCTTTGAGAGCATGGTAACAGAGACTATTCATTCTTAA

Upstream 100 bases:

>100_bases
TTTTTTCATAAAATTACTTCGCAAAAAAAACAAATAAGGGGAACACTAGTCCCCTGTAATACATATAACATTGTCGAACA
TCAAATTACAATATTCAATT

Downstream 100 bases:

>100_bases
AAACGACACACAACATGAGAAACGATATATCCTTGGAATTGGCAAGCCGATTAACGGAACAAGTCAAAACTGCCTTTGAA
AACGGCAGTTTATTAGATGC

Product: adenine-specific DNA methylase

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MPATSLIEELPKIVKEGRQEAQRILERLSSNTHIGLQTNELVLPAKDTSGLWKGKNEQVINKEWMNRLVYGDNLLAMQAL
LAGDEATGLPSLRGKVDLIYIDPPFDSKADYRTKINLPGMDIEQKPTVIEQFAYSDTWQDGTVSYLKMLYPRLVLMRELL
SDKGSIYVHIDWHVGAYVKVLMDDVFGKDNFVNNIIWHYGGPSPIKTAFARKYDIIYLYGKTLKRIFNPQYGNIPISVLN
RAKKDENGRLWLDQNLGKLKEDTIAKMESEGRLYRTKTGGLRRKQYLDEMEGAQFDNVWDIPIINSQAKEDVGYITQKPE
ALLERIIKASSNEGDLVCDFFGGSGTTAAVAERLGRRWITTDIGKPATLVMRKRFIDQEVKPFLYQAIGDYQKEAFQNNK
QYKRIGDLSQIIMQLYGAIPFTQEQLNDRNWGYIKNGRTLVLVDSPNKVTGAATIRRAYEAKNNLLGGGWNKAVVLAWNF
AFDISAAIQQYKEDVEVLVIPPDLLDKLSKKGYDKLIREGSVRFSSYQYLLVKPIQTEPHFGEQDKLTIELDNYVLLSPD
NIPLDDKDKAKLQQVLEKDPLALIEYWSIDPDYDGITFRSQWQDYRENTDNDSDPLHCIYTATLITPRKAERTVCVKAVD
VFGFESMVTETIHS

Sequences:

>Translated_654_residues
MPATSLIEELPKIVKEGRQEAQRILERLSSNTHIGLQTNELVLPAKDTSGLWKGKNEQVINKEWMNRLVYGDNLLAMQAL
LAGDEATGLPSLRGKVDLIYIDPPFDSKADYRTKINLPGMDIEQKPTVIEQFAYSDTWQDGTVSYLKMLYPRLVLMRELL
SDKGSIYVHIDWHVGAYVKVLMDDVFGKDNFVNNIIWHYGGPSPIKTAFARKYDIIYLYGKTLKRIFNPQYGNIPISVLN
RAKKDENGRLWLDQNLGKLKEDTIAKMESEGRLYRTKTGGLRRKQYLDEMEGAQFDNVWDIPIINSQAKEDVGYITQKPE
ALLERIIKASSNEGDLVCDFFGGSGTTAAVAERLGRRWITTDIGKPATLVMRKRFIDQEVKPFLYQAIGDYQKEAFQNNK
QYKRIGDLSQIIMQLYGAIPFTQEQLNDRNWGYIKNGRTLVLVDSPNKVTGAATIRRAYEAKNNLLGGGWNKAVVLAWNF
AFDISAAIQQYKEDVEVLVIPPDLLDKLSKKGYDKLIREGSVRFSSYQYLLVKPIQTEPHFGEQDKLTIELDNYVLLSPD
NIPLDDKDKAKLQQVLEKDPLALIEYWSIDPDYDGITFRSQWQDYRENTDNDSDPLHCIYTATLITPRKAERTVCVKAVD
VFGFESMVTETIHS
>Mature_653_residues
PATSLIEELPKIVKEGRQEAQRILERLSSNTHIGLQTNELVLPAKDTSGLWKGKNEQVINKEWMNRLVYGDNLLAMQALL
AGDEATGLPSLRGKVDLIYIDPPFDSKADYRTKINLPGMDIEQKPTVIEQFAYSDTWQDGTVSYLKMLYPRLVLMRELLS
DKGSIYVHIDWHVGAYVKVLMDDVFGKDNFVNNIIWHYGGPSPIKTAFARKYDIIYLYGKTLKRIFNPQYGNIPISVLNR
AKKDENGRLWLDQNLGKLKEDTIAKMESEGRLYRTKTGGLRRKQYLDEMEGAQFDNVWDIPIINSQAKEDVGYITQKPEA
LLERIIKASSNEGDLVCDFFGGSGTTAAVAERLGRRWITTDIGKPATLVMRKRFIDQEVKPFLYQAIGDYQKEAFQNNKQ
YKRIGDLSQIIMQLYGAIPFTQEQLNDRNWGYIKNGRTLVLVDSPNKVTGAATIRRAYEAKNNLLGGGWNKAVVLAWNFA
FDISAAIQQYKEDVEVLVIPPDLLDKLSKKGYDKLIREGSVRFSSYQYLLVKPIQTEPHFGEQDKLTIELDNYVLLSPDN
IPLDDKDKAKLQQVLEKDPLALIEYWSIDPDYDGITFRSQWQDYRENTDNDSDPLHCIYTATLITPRKAERTVCVKAVDV
FGFESMVTETIHS

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=67, Percent_Identity=55.2238805970149, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 74675; Mature: 74544

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00092 N6_MTASE ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATSLIEELPKIVKEGRQEAQRILERLSSNTHIGLQTNELVLPAKDTSGLWKGKNEQVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCCCCHH
NKEWMNRLVYGDNLLAMQALLAGDEATGLPSLRGKVDLIYIDPPFDSKADYRTKINLPGM
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
DIEQKPTVIEQFAYSDTWQDGTVSYLKMLYPRLVLMRELLSDKGSIYVHIDWHVGAYVKV
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
LMDDVFGKDNFVNNIIWHYGGPSPIKTAFARKYDIIYLYGKTLKRIFNPQYGNIPISVLN
HHHHHCCCCCHHCCEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHCCCCCCCHHHHHH
RAKKDENGRLWLDQNLGKLKEDTIAKMESEGRLYRTKTGGLRRKQYLDEMEGAQFDNVWD
HHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEC
IPIINSQAKEDVGYITQKPEALLERIIKASSNEGDLVCDFFGGSGTTAAVAERLGRRWIT
CCEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEE
TDIGKPATLVMRKRFIDQEVKPFLYQAIGDYQKEAFQNNKQYKRIGDLSQIIMQLYGAIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
FTQEQLNDRNWGYIKNGRTLVLVDSPNKVTGAATIRRAYEAKNNLLGGGWNKAVVLAWNF
CCHHHCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEH
AFDISAAIQQYKEDVEVLVIPPDLLDKLSKKGYDKLIREGSVRFSSYQYLLVKPIQTEPH
HHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHCCCCEEECEEEEEEEEECCCCC
FGEQDKLTIELDNYVLLSPDNIPLDDKDKAKLQQVLEKDPLALIEYWSIDPDYDGITFRS
CCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEHH
QWQDYRENTDNDSDPLHCIYTATLITPRKAERTVCVKAVDVFGFESMVTETIHS
HHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PATSLIEELPKIVKEGRQEAQRILERLSSNTHIGLQTNELVLPAKDTSGLWKGKNEQVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCCCCHH
NKEWMNRLVYGDNLLAMQALLAGDEATGLPSLRGKVDLIYIDPPFDSKADYRTKINLPGM
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
DIEQKPTVIEQFAYSDTWQDGTVSYLKMLYPRLVLMRELLSDKGSIYVHIDWHVGAYVKV
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
LMDDVFGKDNFVNNIIWHYGGPSPIKTAFARKYDIIYLYGKTLKRIFNPQYGNIPISVLN
HHHHHCCCCCHHCCEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHCCCCCCCHHHHHH
RAKKDENGRLWLDQNLGKLKEDTIAKMESEGRLYRTKTGGLRRKQYLDEMEGAQFDNVWD
HHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEC
IPIINSQAKEDVGYITQKPEALLERIIKASSNEGDLVCDFFGGSGTTAAVAERLGRRWIT
CCEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEE
TDIGKPATLVMRKRFIDQEVKPFLYQAIGDYQKEAFQNNKQYKRIGDLSQIIMQLYGAIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
FTQEQLNDRNWGYIKNGRTLVLVDSPNKVTGAATIRRAYEAKNNLLGGGWNKAVVLAWNF
CCHHHCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEH
AFDISAAIQQYKEDVEVLVIPPDLLDKLSKKGYDKLIREGSVRFSSYQYLLVKPIQTEPH
HHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCHHHHHHCCCCEEECEEEEEEEEECCCCC
FGEQDKLTIELDNYVLLSPDNIPLDDKDKAKLQQVLEKDPLALIEYWSIDPDYDGITFRS
CCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEHH
QWQDYRENTDNDSDPLHCIYTATLITPRKAERTVCVKAVDVFGFESMVTETIHS
HHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]