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The map label for this gene is oprM [H]

Identifier: 150009497

GI number: 150009497

Start: 3475757

End: 3477124

Strand: Direct

Name: oprM [H]

Synonym: BDI_2910

Alternate gene names: 150009497

Gene position: 3475757-3477124 (Clockwise)

Preceding gene: 150009496

Following gene: 150009498

Centisome position: 72.24

GC content: 47.22

Gene sequence:

>1368_bases
ATGAAACGACATATCATCACACTAGCTGTATCATGCGTTATGTTAAACAGCTGTGGTATATATACAAAATACAAACCGGC
TTCTGAGGTTTCCGCAGATCTGTACGGCTCAGAGGCCACCGCTCAAACGGATACTGTATCCTTGGGAGACATGAGCTGGC
GGGAGGTCTTCACCGACCCGCAGCTCCAAACACTCATTGAGCATGGCCTAGCAAACAACACAGACTATCGTTCCGCACAA
CTCCGTGTAGAGGAGGCTGAAGCAGCTTTACTGTCAGCCAAGCTAGCTTTCCTGCCTGCCTTCGCTTTCGCTCCTCAAGG
AGCGGTGAGCAGTTTCGACTCCCATAAGGCTACACAGACTTATTCGATTCCGATAACAGCCAGTTGGGAACTGGATATAT
TTGGTAAAATGCGCAATGCCAAAAAGCAGGCGCAAGCTCTTTACGCACAAAGTCAAGATTATCGGCAAGCGGTACGTACA
CAGCTAATCGCCGGAATAGCTAATACTTATTATTCCCTGCTTATGCTGGATGCTCAGTTGAAGATCTCAGAGCAAACCGC
TTCCTCCTGGAAAGAGACGGTCAATTCCACCCGTGCCTTAATGAATGCAGGCATAGTAAACGAAACAGCTGTTTCCCAAA
TGGAAGCTACGTACTACAGCATCTGTACATCCATCCTCGACCTGAAAGAACAAATCAATCAGGTAGAGAACAGCCTTGTA
TTGTTACTGGCTGATACACCTCATACCATACAAAGAGGTGAATTGGAAAACCAGCAACTGCCGAAACATTTCTCAGTAGG
CATACCCGTCTATTTATTATCAAACCGCCCTGATGTAAGGGCAGCGGAACATGCTCTGGAAAGTGCATTTTACGCCACTA
ATCAAGCCCGTTCCGCTTTTTATCCCTCTATTACCCTAAGTGGTTCGGCCGGCTGGACTAACTCGGCAGGTGCAGTCATC
ACCAATCCGGGTAAGTTTCTGGCCTCGGCAGTAGGCTCACTCACCCAGCCACTTTTTGCCAGAGGACAACTTCTAGGACA
ACTGAAAATCACCAAAGCGCAACAAGAAGAGGCCCGGTTGAGTTTTGAGCAAGCTTTACTGAACGCAGGAACCGAGGTTA
ACGATGCTTTGGTGCAATATCATACAGCACGTGACAAGGCTGTGTACTTTGAAAAACAGATAGAGTCTCTCGAAAGAGCC
TATAAAAGTACCTCCCTACTTATGCAGCACGGAACGACAACCTATCTAGAAGTACTCACAGCACAACAAGGATTATTAAA
TGCCCAACTAACCCAAGTGGCTAATCGTTTCACGGAAATCCAAGGGGTTATTAACCTCTATCAAGCCTTAGGCGGAGGAC
GTGAATAG

Upstream 100 bases:

>100_bases
CAGACCTACAGGTATTGCAAGAGCAAGAAAAGAGCGCAAACGAACGCGAGAATCATAAATAAATCGGGAGAATCCCATTA
ATTACAATTATAGAAAAAGA

Downstream 100 bases:

>100_bases
GCCATTCGTTTTATTCATATTTGAAATAACCTATAGGATTGCCCAGAAAGTGATTTTAGGGCAATCCGCCTTTCTTAGCA
CGCTCTCACTTTCACCTAAT

Product: putative multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKRHIITLAVSCVMLNSCGIYTKYKPASEVSADLYGSEATAQTDTVSLGDMSWREVFTDPQLQTLIEHGLANNTDYRSAQ
LRVEEAEAALLSAKLAFLPAFAFAPQGAVSSFDSHKATQTYSIPITASWELDIFGKMRNAKKQAQALYAQSQDYRQAVRT
QLIAGIANTYYSLLMLDAQLKISEQTASSWKETVNSTRALMNAGIVNETAVSQMEATYYSICTSILDLKEQINQVENSLV
LLLADTPHTIQRGELENQQLPKHFSVGIPVYLLSNRPDVRAAEHALESAFYATNQARSAFYPSITLSGSAGWTNSAGAVI
TNPGKFLASAVGSLTQPLFARGQLLGQLKITKAQQEEARLSFEQALLNAGTEVNDALVQYHTARDKAVYFEKQIESLERA
YKSTSLLMQHGTTTYLEVLTAQQGLLNAQLTQVANRFTEIQGVINLYQALGGGRE

Sequences:

>Translated_455_residues
MKRHIITLAVSCVMLNSCGIYTKYKPASEVSADLYGSEATAQTDTVSLGDMSWREVFTDPQLQTLIEHGLANNTDYRSAQ
LRVEEAEAALLSAKLAFLPAFAFAPQGAVSSFDSHKATQTYSIPITASWELDIFGKMRNAKKQAQALYAQSQDYRQAVRT
QLIAGIANTYYSLLMLDAQLKISEQTASSWKETVNSTRALMNAGIVNETAVSQMEATYYSICTSILDLKEQINQVENSLV
LLLADTPHTIQRGELENQQLPKHFSVGIPVYLLSNRPDVRAAEHALESAFYATNQARSAFYPSITLSGSAGWTNSAGAVI
TNPGKFLASAVGSLTQPLFARGQLLGQLKITKAQQEEARLSFEQALLNAGTEVNDALVQYHTARDKAVYFEKQIESLERA
YKSTSLLMQHGTTTYLEVLTAQQGLLNAQLTQVANRFTEIQGVINLYQALGGGRE
>Mature_455_residues
MKRHIITLAVSCVMLNSCGIYTKYKPASEVSADLYGSEATAQTDTVSLGDMSWREVFTDPQLQTLIEHGLANNTDYRSAQ
LRVEEAEAALLSAKLAFLPAFAFAPQGAVSSFDSHKATQTYSIPITASWELDIFGKMRNAKKQAQALYAQSQDYRQAVRT
QLIAGIANTYYSLLMLDAQLKISEQTASSWKETVNSTRALMNAGIVNETAVSQMEATYYSICTSILDLKEQINQVENSLV
LLLADTPHTIQRGELENQQLPKHFSVGIPVYLLSNRPDVRAAEHALESAFYATNQARSAFYPSITLSGSAGWTNSAGAVI
TNPGKFLASAVGSLTQPLFARGQLLGQLKITKAQQEEARLSFEQALLNAGTEVNDALVQYHTARDKAVYFEKQIESLERA
YKSTSLLMQHGTTTYLEVLTAQQGLLNAQLTQVANRFTEIQGVINLYQALGGGRE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=452, Percent_Identity=28.3185840707965, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI2367348, Length=426, Percent_Identity=23.7089201877934, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49908; Mature: 49908

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHIITLAVSCVMLNSCGIYTKYKPASEVSADLYGSEATAQTDTVSLGDMSWREVFTDP
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCH
QLQTLIEHGLANNTDYRSAQLRVEEAEAALLSAKLAFLPAFAFAPQGAVSSFDSHKATQT
HHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEE
YSIPITASWELDIFGKMRNAKKQAQALYAQSQDYRQAVRTQLIAGIANTYYSLLMLDAQL
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISEQTASSWKETVNSTRALMNAGIVNETAVSQMEATYYSICTSILDLKEQINQVENSLV
EEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLLADTPHTIQRGELENQQLPKHFSVGIPVYLLSNRPDVRAAEHALESAFYATNQARSAF
EEEECCCCCHHCCCCCCCCCCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHC
YPSITLSGSAGWTNSAGAVITNPGKFLASAVGSLTQPLFARGQLLGQLKITKAQQEEARL
CCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
SFEQALLNAGTEVNDALVQYHTARDKAVYFEKQIESLERAYKSTSLLMQHGTTTYLEVLT
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AQQGLLNAQLTQVANRFTEIQGVINLYQALGGGRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRHIITLAVSCVMLNSCGIYTKYKPASEVSADLYGSEATAQTDTVSLGDMSWREVFTDP
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCH
QLQTLIEHGLANNTDYRSAQLRVEEAEAALLSAKLAFLPAFAFAPQGAVSSFDSHKATQT
HHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEE
YSIPITASWELDIFGKMRNAKKQAQALYAQSQDYRQAVRTQLIAGIANTYYSLLMLDAQL
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISEQTASSWKETVNSTRALMNAGIVNETAVSQMEATYYSICTSILDLKEQINQVENSLV
EEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLLADTPHTIQRGELENQQLPKHFSVGIPVYLLSNRPDVRAAEHALESAFYATNQARSAF
EEEECCCCCHHCCCCCCCCCCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHC
YPSITLSGSAGWTNSAGAVITNPGKFLASAVGSLTQPLFARGQLLGQLKITKAQQEEARL
CCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
SFEQALLNAGTEVNDALVQYHTARDKAVYFEKQIESLERAYKSTSLLMQHGTTTYLEVLT
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AQQGLLNAQLTQVANRFTEIQGVINLYQALGGGRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]