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The map label for this gene is pfkA3 [H]

Identifier: 150009422

GI number: 150009422

Start: 3375552

End: 3376565

Strand: Reverse

Name: pfkA3 [H]

Synonym: BDI_2830

Alternate gene names: 150009422

Gene position: 3376565-3375552 (Counterclockwise)

Preceding gene: 150009424

Following gene: 150009418

Centisome position: 70.18

GC content: 49.01

Gene sequence:

>1014_bases
ATGAAAATAGGGATTCTCTCATCGGGTGGCGATTGCCCCGGTATCAACGCTACGATCCGTGGTGTTGGCAAGACCGCTAT
CATGCATTACGGCATGGAAGTGATAGGTATTCATAGCGGTTTTATAGGTCTTTTGAATAAAGACGTCCAAGTTTTCGATG
AACGGAGCTTATCCGGCATATTAAATTTAGGGGGAACCATTTTAGGAACGTCCAGAGAGAAGCCTTTCCGTAAGCTTTTG
GCCTCGGGAGATTCGGAAAAGCCGGAGATCATTAAGAAAAATTATGAGGAGCTGGGGCTGGACTGCTTGGTTTGTATCGG
AGGTAACGGTACGCAGAAGACGGCCAATCTGTTATCTCAGATCGGATTGAACGTGATAGGCGTTCCCAAGACAATCGATA
ATGACATATGGGGTACGGATATTACGTTCGGTTTTAATACCGCCGTGAATATCGCCACGGAATCTATAGACCGTCTTCAT
TCTACGGCTAGTTCACATAAGCGGGTGATGGTCGTGGAGGTGATGGGCCATCATGCCGGATGGATCGCCTTGTATGCCGG
GATGGCTGGAGGTGCCGACGTGATCTTACTTCCGGAATTGGGATACGATATCGATAAGGTGAACGAGGCGATCTTGGATC
GGGCTCGCAGGGGCAAACCTTATTCCATCGTTGTAGTAGCCGAGGGTATCGAGACTCCGGATAAAAAGCGTCCTTCTGAT
TATATCACCCGGGCTATCGAGGCGGGAACGGGTATCGAGACCCGGGATACCGTATTGGGATACACCCAACGGGGTGGAAA
CCCCTCTCCGTTCGACCGTAACCTCGCTACCCGCTTGGGAGGCCATGCCACGGAACTGATCGCTAATGGTGAATTCGGAC
GTATGGTTTGTATAAAAGGAGATAGGGTAGAATCCATTCCTTTGTTAGAAGTAGCGGGAAAACTAAAATTAGTGACACCG
GATCATGATTTGATCGTACAAGGAAAGCGGATGGGGGTAACATTCGGGAAATAA

Upstream 100 bases:

>100_bases
GTCTGTGTTAAAATATCCATGCCCTTTTTATTTGTTCGAGTGCAAATGTAGGCATTCTCTTTTTATTTATATCTTTGCTA
AGTACTAAATTAACGTTACG

Downstream 100 bases:

>100_bases
AATAAAAAAGGTTGTGCGTTCTCTGACACACAACCTTTTTTACTTTATACTAGTAGGCGGCCGTTTATTTCTTAATACCT
TGAACGGTTTGGCTTCCCTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKIGILSSGGDCPGINATIRGVGKTAIMHYGMEVIGIHSGFIGLLNKDVQVFDERSLSGILNLGGTILGTSREKPFRKLL
ASGDSEKPEIIKKNYEELGLDCLVCIGGNGTQKTANLLSQIGLNVIGVPKTIDNDIWGTDITFGFNTAVNIATESIDRLH
STASSHKRVMVVEVMGHHAGWIALYAGMAGGADVILLPELGYDIDKVNEAILDRARRGKPYSIVVVAEGIETPDKKRPSD
YITRAIEAGTGIETRDTVLGYTQRGGNPSPFDRNLATRLGGHATELIANGEFGRMVCIKGDRVESIPLLEVAGKLKLVTP
DHDLIVQGKRMGVTFGK

Sequences:

>Translated_337_residues
MKIGILSSGGDCPGINATIRGVGKTAIMHYGMEVIGIHSGFIGLLNKDVQVFDERSLSGILNLGGTILGTSREKPFRKLL
ASGDSEKPEIIKKNYEELGLDCLVCIGGNGTQKTANLLSQIGLNVIGVPKTIDNDIWGTDITFGFNTAVNIATESIDRLH
STASSHKRVMVVEVMGHHAGWIALYAGMAGGADVILLPELGYDIDKVNEAILDRARRGKPYSIVVVAEGIETPDKKRPSD
YITRAIEAGTGIETRDTVLGYTQRGGNPSPFDRNLATRLGGHATELIANGEFGRMVCIKGDRVESIPLLEVAGKLKLVTP
DHDLIVQGKRMGVTFGK
>Mature_337_residues
MKIGILSSGGDCPGINATIRGVGKTAIMHYGMEVIGIHSGFIGLLNKDVQVFDERSLSGILNLGGTILGTSREKPFRKLL
ASGDSEKPEIIKKNYEELGLDCLVCIGGNGTQKTANLLSQIGLNVIGVPKTIDNDIWGTDITFGFNTAVNIATESIDRLH
STASSHKRVMVVEVMGHHAGWIALYAGMAGGADVILLPELGYDIDKVNEAILDRARRGKPYSIVVVAEGIETPDKKRPSD
YITRAIEAGTGIETRDTVLGYTQRGGNPSPFDRNLATRLGGHATELIANGEFGRMVCIKGDRVESIPLLEVAGKLKLVTP
DHDLIVQGKRMGVTFGK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=342, Percent_Identity=35.3801169590643, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI48762920, Length=354, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI266453619, Length=356, Percent_Identity=33.7078651685393, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI266453768, Length=356, Percent_Identity=33.7078651685393, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI266453748, Length=356, Percent_Identity=33.7078651685393, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4505749, Length=356, Percent_Identity=33.7078651685393, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1790350, Length=315, Percent_Identity=40.9523809523809, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI25147584, Length=350, Percent_Identity=31.1428571428571, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17558788, Length=350, Percent_Identity=29.1428571428571, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI72003601, Length=300, Percent_Identity=27, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321679, Length=346, Percent_Identity=34.1040462427746, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323861, Length=351, Percent_Identity=31.3390313390313, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24652337, Length=351, Percent_Identity=32.1937321937322, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17647809, Length=350, Percent_Identity=32.2857142857143, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI28573326, Length=351, Percent_Identity=32.1937321937322, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36076; Mature: 36076

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSSGGDCPGINATIRGVGKTAIMHYGMEVIGIHSGFIGLLNKDVQVFDERSLSGI
CEEEEECCCCCCCCCCCEEECCCHHHHHHCCCEEEEECCCHHHHHCCCHHHHHHHHHHHH
LNLGGTILGTSREKPFRKLLASGDSEKPEIIKKNYEELGLDCLVCIGGNGTQKTANLLSQ
HHCCCEEEECCCCHHHHHHHHCCCCCCCHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHH
IGLNVIGVPKTIDNDIWGTDITFGFNTAVNIATESIDRLHSTASSHKRVMVVEVMGHHAG
HCCCEEECCCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
WIALYAGMAGGADVILLPELGYDIDKVNEAILDRARRGKPYSIVVVAEGIETPDKKRPSD
EEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHH
YITRAIEAGTGIETRDTVLGYTQRGGNPSPFDRNLATRLGGHATELIANGEFGRMVCIKG
HHHHHHHCCCCCCCCHHEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEEEC
DRVESIPLLEVAGKLKLVTPDHDLIVQGKRMGVTFGK
CCCCCCCCEEECCCEEEECCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKIGILSSGGDCPGINATIRGVGKTAIMHYGMEVIGIHSGFIGLLNKDVQVFDERSLSGI
CEEEEECCCCCCCCCCCEEECCCHHHHHHCCCEEEEECCCHHHHHCCCHHHHHHHHHHHH
LNLGGTILGTSREKPFRKLLASGDSEKPEIIKKNYEELGLDCLVCIGGNGTQKTANLLSQ
HHCCCEEEECCCCHHHHHHHHCCCCCCCHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHH
IGLNVIGVPKTIDNDIWGTDITFGFNTAVNIATESIDRLHSTASSHKRVMVVEVMGHHAG
HCCCEEECCCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
WIALYAGMAGGADVILLPELGYDIDKVNEAILDRARRGKPYSIVVVAEGIETPDKKRPSD
EEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHH
YITRAIEAGTGIETRDTVLGYTQRGGNPSPFDRNLATRLGGHATELIANGEFGRMVCIKG
HHHHHHHCCCCCCCCHHEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEEEC
DRVESIPLLEVAGKLKLVTPDHDLIVQGKRMGVTFGK
CCCCCCCCEEECCCEEEECCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]