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The map label for this gene is purN [H]

Identifier: 150009418

GI number: 150009418

Start: 3369728

End: 3370288

Strand: Reverse

Name: purN [H]

Synonym: BDI_2826

Alternate gene names: 150009418

Gene position: 3370288-3369728 (Counterclockwise)

Preceding gene: 150009422

Following gene: 150009414

Centisome position: 70.05

GC content: 45.81

Gene sequence:

>561_bases
ATGAAAAACATAGCGATTTTTGCTTCCGGATCTGGTACGAATGCCGAGAACATCACCCGATATTTTGCTAACAGTGAAAA
CGTTAATGTTGCGGTTGTTTTATCAAATAACCGCAATGTTGGCGTGCATGGGCGGGTAAACAAGTTGGGCGTTCCTTCTT
TCGTGTTTTCACGGGAGGAATTTATTGCCGGTGTGCCGATCTTGAAAAAGTTGGCGGAATATGATGTCTGCCTTATCGTG
TTGGCCGGTTTTATGAATAAGATCTCAGACGTTATTCTTCAGGCTTTTCCCGGAAAGATCGTGAACATCCATCCTGCGTT
ACTCCCTAAATATGGCGGCAAGGGGATGTATGGCATGCATGTGCATGAGGCGGTCGTAAAGGCTGGTGAGCGGGAGTCGG
GTATTACTATACATTATATTAATGAGCATTATGATGAGGGCGCTATCATCTTCCAGGCTTCTTGCCCGGTATTACCTTCG
GATACGCCAGATGAGGTAGCCGCGAAAGTGCATGCCTTGGAGTATGCGCATTATCCTCATGTGATCGAAAGTTTACTGTA
A

Upstream 100 bases:

>100_bases
AAAGAAAAGAATTTTTTATGTTACAAAGCAAATTTAATAAGCTAAAAATGCGGAAAACTGCACATTTTTTCTTTTCTTGT
GCATTTAAACATCTGAAAAT

Downstream 100 bases:

>100_bases
GGCTTTTTTGTCATTAGCCTACTTGAAAACCTAGTTTTTGGAGTACGGCGAATTCTTCCGGGTCAGCTTTGATCGCTTGG
TAAGCATGGAACTCACGGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKNIAIFASGSGTNAENITRYFANSENVNVAVVLSNNRNVGVHGRVNKLGVPSFVFSREEFIAGVPILKKLAEYDVCLIV
LAGFMNKISDVILQAFPGKIVNIHPALLPKYGGKGMYGMHVHEAVVKAGERESGITIHYINEHYDEGAIIFQASCPVLPS
DTPDEVAAKVHALEYAHYPHVIESLL

Sequences:

>Translated_186_residues
MKNIAIFASGSGTNAENITRYFANSENVNVAVVLSNNRNVGVHGRVNKLGVPSFVFSREEFIAGVPILKKLAEYDVCLIV
LAGFMNKISDVILQAFPGKIVNIHPALLPKYGGKGMYGMHVHEAVVKAGERESGITIHYINEHYDEGAIIFQASCPVLPS
DTPDEVAAKVHALEYAHYPHVIESLL
>Mature_186_residues
MKNIAIFASGSGTNAENITRYFANSENVNVAVVLSNNRNVGVHGRVNKLGVPSFVFSREEFIAGVPILKKLAEYDVCLIV
LAGFMNKISDVILQAFPGKIVNIHPALLPKYGGKGMYGMHVHEAVVKAGERESGITIHYINEHYDEGAIIFQASCPVLPS
DTPDEVAAKVHALEYAHYPHVIESLL

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1788846, Length=187, Percent_Identity=31.5508021390374, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=182, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=36.4583333333333, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20258; Mature: 20258

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIAIFASGSGTNAENITRYFANSENVNVAVVLSNNRNVGVHGRVNKLGVPSFVFSREE
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCHHH
FIAGVPILKKLAEYDVCLIVLAGFMNKISDVILQAFPGKIVNIHPALLPKYGGKGMYGMH
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCCCCHHH
VHEAVVKAGERESGITIHYINEHYDEGAIIFQASCPVLPSDTPDEVAAKVHALEYAHYPH
HHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VIESLL
HHHHHC
>Mature Secondary Structure
MKNIAIFASGSGTNAENITRYFANSENVNVAVVLSNNRNVGVHGRVNKLGVPSFVFSREE
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCHHH
FIAGVPILKKLAEYDVCLIVLAGFMNKISDVILQAFPGKIVNIHPALLPKYGGKGMYGMH
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCCCCHHH
VHEAVVKAGERESGITIHYINEHYDEGAIIFQASCPVLPSDTPDEVAAKVHALEYAHYPH
HHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VIESLL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA