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The map label for this gene is lacA [C]

Identifier: 150009375

GI number: 150009375

Start: 3320377

End: 3320922

Strand: Reverse

Name: lacA [C]

Synonym: BDI_2783

Alternate gene names: 150009375

Gene position: 3320922-3320377 (Counterclockwise)

Preceding gene: 150009376

Following gene: 150009374

Centisome position: 69.02

GC content: 39.74

Gene sequence:

>546_bases
ATGTTTATGGATTCTTTTTATTCACGATCTGAATTAGAACATATAGGTTTTGCTACCATAGGCAAGAATGTACTGTTGAG
CCGTAAGGCTAGTATCTATTCTCCCGCTATGATATCGATTGGAGATAATGTGAGGATTGATGACTTTTGTATCCTGTCCG
GTTGTATTGGAATTGGTTCTCAAGTTCATATTTCCGCTTACGTGGCTTTATATGGAGGTATGGGAATCACAATTGAAGAT
TATTCAGGTGTATCGGCAAGGACTACAATTTATAGTGCTGTTGATGATTTTGGAGGAGATTATCTGATTGGGCCAATGTG
TCCGGTAGAAATGACACATGTGATGGGAGGTCCGGTTGTTTTGCAAAAATATGTTCAAGTGGGGGCTTCTTGCGTAGTAA
TGCCGAGCTTAACAGTTGCGGAGGGTAGTGTCGTGGGAGCAATGAGTTTTGTTAATCAATCCTTGGCTTCATGGGGCATT
TATGCTGGAGTACCGGTAAGAAGATTGCGAGATCGAAGCAAAAAATTATTAAAATTTACAAAATAA

Upstream 100 bases:

>100_bases
GAAGCGTATAAGATGGCTGAGCGGGTGATTTGTTTGCCTATGCACCATGCTTTGAGTGAGGATGATGTAGAGCGAGTGAT
AGGTCAAATAATAGAGTAAG

Downstream 100 bases:

>100_bases
CTTATATGTCAGAAGGTAATTACGCACCTATCATTGTTTCTGTATATGATCGTTTGGATCATTTGAAAAGGAATATAAAG
GCATTGTTAAACAATAGATT

Product: putative galactoside O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Transferase Hexapeptide Repeat Containing Protein; Hexapaptide Repeat-Containing Transferase; TDP-4-Oxo-6-Deoxy-D-Glucose Transaminase; Galactoside O-Acetyltransferase; O-Acetyltransferase; Acetyltransferase RfbO CysE/LacA/LpxA/NodL Family; Lipopolysaccharide Biosynthesis O-Acetyl Transferase; LPS Biosynthesis O-Acetyl Transferase; CysE/LacA/LpxA/NodL Family O-Acyltransferase; DTDP-Viosamine N-Acetyltransferase; Pseudogene

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MFMDSFYSRSELEHIGFATIGKNVLLSRKASIYSPAMISIGDNVRIDDFCILSGCIGIGSQVHISAYVALYGGMGITIED
YSGVSARTTIYSAVDDFGGDYLIGPMCPVEMTHVMGGPVVLQKYVQVGASCVVMPSLTVAEGSVVGAMSFVNQSLASWGI
YAGVPVRRLRDRSKKLLKFTK

Sequences:

>Translated_181_residues
MFMDSFYSRSELEHIGFATIGKNVLLSRKASIYSPAMISIGDNVRIDDFCILSGCIGIGSQVHISAYVALYGGMGITIED
YSGVSARTTIYSAVDDFGGDYLIGPMCPVEMTHVMGGPVVLQKYVQVGASCVVMPSLTVAEGSVVGAMSFVNQSLASWGI
YAGVPVRRLRDRSKKLLKFTK
>Mature_181_residues
MFMDSFYSRSELEHIGFATIGKNVLLSRKASIYSPAMISIGDNVRIDDFCILSGCIGIGSQVHISAYVALYGGMGITIED
YSGVSARTTIYSAVDDFGGDYLIGPMCPVEMTHVMGGPVVLQKYVQVGASCVVMPSLTVAEGSVVGAMSFVNQSLASWGI
YAGVPVRRLRDRSKKLLKFTK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 19455; Mature: 19455

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMDSFYSRSELEHIGFATIGKNVLLSRKASIYSPAMISIGDNVRIDDFCILSGCIGIGS
CCCCCHHCHHHHHHCCHHHHCCHHHHHCCCCCCCCEEEEECCCCCCCHHHEEHHHHCCCC
QVHISAYVALYGGMGITIEDYSGVSARTTIYSAVDDFGGDYLIGPMCPVEMTHVMGGPVV
EEHHEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCHHH
LQKYVQVGASCVVMPSLTVAEGSVVGAMSFVNQSLASWGIYAGVPVRRLRDRSKKLLKFT
HHHHHHHCCCEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MFMDSFYSRSELEHIGFATIGKNVLLSRKASIYSPAMISIGDNVRIDDFCILSGCIGIGS
CCCCCHHCHHHHHHCCHHHHCCHHHHHCCCCCCCCEEEEECCCCCCCHHHEEHHHHCCCC
QVHISAYVALYGGMGITIEDYSGVSARTTIYSAVDDFGGDYLIGPMCPVEMTHVMGGPVV
EEHHEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCHHH
LQKYVQVGASCVVMPSLTVAEGSVVGAMSFVNQSLASWGIYAGVPVRRLRDRSKKLLKFT
HHHHHHHCCCEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA