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The map label for this gene is ispF

Identifier: 150009172

GI number: 150009172

Start: 3047326

End: 3047811

Strand: Reverse

Name: ispF

Synonym: BDI_2574

Alternate gene names: 150009172

Gene position: 3047811-3047326 (Counterclockwise)

Preceding gene: 150009173

Following gene: 150009170

Centisome position: 63.35

GC content: 52.26

Gene sequence:

>486_bases
ATGAAGATACGTGTAGGTTTTGGCTATGATGTCCACGCCTTGGTGCCGGACCGGGAATTATGGCTAGGCGGGATTAAGAT
CGAGCATACGTTGGGCCTATTGGGACATAGCGATGCGGACGTGTTGATCCATGCGATCTGCGACGCTTTACTGGGAGCGG
CCAATATGCGGGATATCGGTTATCATTTCCCGGATACGGCTGGTGAATATAAGAATATCGATAGTAAGATCTTGCTCCGG
GATACCATGAGGCTTTTAAGAGAGGCGGGCTATGAGTTGGGTAATATCGATGCCACCGTAGCCGCCGAGCGCCCTAAATT
GAATCCCCATATCCCTCTGATGAAGAAAACGCTGGCCGAGGTCATGAACGTGGACGAGGAGGATATCTCTATCAAGGCTA
CGACCACGGAGAAGCTGGGCTTCACCGGACGGCAGGAAGGGATCTCGGCTTACGCTACGGTCTTGATACAGCGCATGGGA
TCGTAA

Upstream 100 bases:

>100_bases
TCTGATCAGTACGGTACAGAGTAACCCGTTGGACCAGACATTGCGCTTCTCCTTGTCGTTCGATATGGATGGAATCAAGA
ACCTTTTCAGATAAATGGAT

Downstream 100 bases:

>100_bases
ACCAGAAAGTACTCCCTTTTCCTTCTTCCGACTCTACGCCGATCTTCCCGTGAAGATTCTCTAGGATCGTTTGGCAAATG
GATAGTCCGAGTCCGGTTCC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKIRVGFGYDVHALVPDRELWLGGIKIEHTLGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEYKNIDSKILLR
DTMRLLREAGYELGNIDATVAAERPKLNPHIPLMKKTLAEVMNVDEEDISIKATTTEKLGFTGRQEGISAYATVLIQRMG
S

Sequences:

>Translated_161_residues
MKIRVGFGYDVHALVPDRELWLGGIKIEHTLGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEYKNIDSKILLR
DTMRLLREAGYELGNIDATVAAERPKLNPHIPLMKKTLAEVMNVDEEDISIKATTTEKLGFTGRQEGISAYATVLIQRMG
S
>Mature_161_residues
MKIRVGFGYDVHALVPDRELWLGGIKIEHTLGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEYKNIDSKILLR
DTMRLLREAGYELGNIDATVAAERPKLNPHIPLMKKTLAEVMNVDEEDISIKATTTEKLGFTGRQEGISAYATVLIQRMG
S

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=50, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PARD8 (A6LF29)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303915.1
- ProteinModelPortal:   A6LF29
- SMR:   A6LF29
- STRING:   A6LF29
- GeneID:   5307723
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2574
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   PDIS435591:BDI_2574-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17744; Mature: 17744

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRVGFGYDVHALVPDRELWLGGIKIEHTLGLLGHSDADVLIHAICDALLGAANMRDIG
CEEEEECCCCEEEECCCCCEEECCEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YHFPDTAGEYKNIDSKILLRDTMRLLREAGYELGNIDATVAAERPKLNPHIPLMKKTLAE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
VMNVDEEDISIKATTTEKLGFTGRQEGISAYATVLIQRMGS
HHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIRVGFGYDVHALVPDRELWLGGIKIEHTLGLLGHSDADVLIHAICDALLGAANMRDIG
CEEEEECCCCEEEECCCCCEEECCEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YHFPDTAGEYKNIDSKILLRDTMRLLREAGYELGNIDATVAAERPKLNPHIPLMKKTLAE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
VMNVDEEDISIKATTTEKLGFTGRQEGISAYATVLIQRMGS
HHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA