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The map label for this gene is spsK [H]

Identifier: 150009110

GI number: 150009110

Start: 2959648

End: 2960511

Strand: Reverse

Name: spsK [H]

Synonym: BDI_2511

Alternate gene names: 150009110

Gene position: 2960511-2959648 (Counterclockwise)

Preceding gene: 150009111

Following gene: 150009109

Centisome position: 61.53

GC content: 48.15

Gene sequence:

>864_bases
ATGAAGACAGTTTTGGTAACCGGAGCGAACGGTCAATTGGGAAATTCTATCCACGCTCGTATCAGCCAGTATCCCGGCTA
TAATTTTCTTTTTACGGATATAGATACGTTGGATATTTGCGACAAGGAGGCCGTCCGGAAATATGTCTTGGAAAATGATG
TTCAATATGTCATGAATTGCGCCGCTTATACGGCGGTGGATAAAGCGGAGGAGCATGAGGATCTGTGTATGCGCATCAAC
CGGGATGCGGTACGGAACTTAGGTGAGGCCGCTCATGCCGTGGGTGCGAAGGTGATTCATGTATCTACCGATTATGTGTT
CGATGGAACGAGTTGCCGTCCTTATCTGGAAAGCGATGCGACTTGTCCTGTTTCCGTATATGGACGTACGAAACTGGCGG
GAGAGCAGGCCTTGATGGAGGTGTGTCCGGATTCCGTGATTATCCGTACGGCTTGGTTGTATTCCGAGTATGGAAACAAT
TTTATGAAGACGGTATTGCGTTTGGGAAAGGAGCGGGATGAGTTGCGTTTCATATTCGACCAAGTGGGTACGCCTACTTA
TGCCGGAGATTTAGCGGTCGCTATGCTTGCCGTGCTGGAACGTGCCGAGGCGGGTAAATTTATACCGGGTATCTATCATT
TCTCGGACGAGGGGGTGTGTAGCTGGTATGATTTTACGGTAAAGATCTTGCAAATAGCAGGTATGAATAACCGGGTGATA
CCCATAGAGACCAAGGATTATCCGACACCGGCCAGCCGGCCGCATTATAGCGTATTGAATAAAGGTAAGATAAAAAGCAC
GTACGGACTGACGATTCCCCATTGGGAGACTAGCTTGGCCTACTGCATGAATAACAAAGTGTAA

Upstream 100 bases:

>100_bases
GTATTAAACAAATCAGTTCCTTCTTGGCGTTGCTCGCCGAGAAATATCGGGAAGATATGAAAGGGGAACTTAAACTAGAG
GATTAATAATAGATATAGAC

Downstream 100 bases:

>100_bases
CTGATAACAATATATACATATAAAATAGAATGAGCCAGTATTCAGAAGAGATTGAAAGAAGAAGAACGTTTGCCATAATC
AGCCACCCGGATGCCGGTAA

Product: putative dTDP-4-dehydrorhamnose reductase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKTVLVTGANGQLGNSIHARISQYPGYNFLFTDIDTLDICDKEAVRKYVLENDVQYVMNCAAYTAVDKAEEHEDLCMRIN
RDAVRNLGEAAHAVGAKVIHVSTDYVFDGTSCRPYLESDATCPVSVYGRTKLAGEQALMEVCPDSVIIRTAWLYSEYGNN
FMKTVLRLGKERDELRFIFDQVGTPTYAGDLAVAMLAVLERAEAGKFIPGIYHFSDEGVCSWYDFTVKILQIAGMNNRVI
PIETKDYPTPASRPHYSVLNKGKIKSTYGLTIPHWETSLAYCMNNKV

Sequences:

>Translated_287_residues
MKTVLVTGANGQLGNSIHARISQYPGYNFLFTDIDTLDICDKEAVRKYVLENDVQYVMNCAAYTAVDKAEEHEDLCMRIN
RDAVRNLGEAAHAVGAKVIHVSTDYVFDGTSCRPYLESDATCPVSVYGRTKLAGEQALMEVCPDSVIIRTAWLYSEYGNN
FMKTVLRLGKERDELRFIFDQVGTPTYAGDLAVAMLAVLERAEAGKFIPGIYHFSDEGVCSWYDFTVKILQIAGMNNRVI
PIETKDYPTPASRPHYSVLNKGKIKSTYGLTIPHWETSLAYCMNNKV
>Mature_287_residues
MKTVLVTGANGQLGNSIHARISQYPGYNFLFTDIDTLDICDKEAVRKYVLENDVQYVMNCAAYTAVDKAEEHEDLCMRIN
RDAVRNLGEAAHAVGAKVIHVSTDYVFDGTSCRPYLESDATCPVSVYGRTKLAGEQALMEVCPDSVIIRTAWLYSEYGNN
FMKTVLRLGKERDELRFIFDQVGTPTYAGDLAVAMLAVLERAEAGKFIPGIYHFSDEGVCSWYDFTVKILQIAGMNNRVI
PIETKDYPTPASRPHYSVLNKGKIKSTYGLTIPHWETSLAYCMNNKV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=287, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI11034825, Length=287, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=40.2061855670103, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 32100; Mature: 32100

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVLVTGANGQLGNSIHARISQYPGYNFLFTDIDTLDICDKEAVRKYVLENDVQYVMNC
CCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
AAYTAVDKAEEHEDLCMRINRDAVRNLGEAAHAVGAKVIHVSTDYVFDGTSCRPYLESDA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCC
TCPVSVYGRTKLAGEQALMEVCPDSVIIRTAWLYSEYGNNFMKTVLRLGKERDELRFIFD
CCCEEECCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
QVGTPTYAGDLAVAMLAVLERAEAGKFIPGIYHFSDEGVCSWYDFTVKILQIAGMNNRVI
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHHHEEEHHEECCCCCEE
PIETKDYPTPASRPHYSVLNKGKIKSTYGLTIPHWETSLAYCMNNKV
EEECCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKTVLVTGANGQLGNSIHARISQYPGYNFLFTDIDTLDICDKEAVRKYVLENDVQYVMNC
CCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
AAYTAVDKAEEHEDLCMRINRDAVRNLGEAAHAVGAKVIHVSTDYVFDGTSCRPYLESDA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCC
TCPVSVYGRTKLAGEQALMEVCPDSVIIRTAWLYSEYGNNFMKTVLRLGKERDELRFIFD
CCCEEECCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
QVGTPTYAGDLAVAMLAVLERAEAGKFIPGIYHFSDEGVCSWYDFTVKILQIAGMNNRVI
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHHHEEEHHEECCCCCEE
PIETKDYPTPASRPHYSVLNKGKIKSTYGLTIPHWETSLAYCMNNKV
EEECCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]