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The map label for this gene is cls [H]

Identifier: 150009099

GI number: 150009099

Start: 2948857

End: 2950293

Strand: Direct

Name: cls [H]

Synonym: BDI_2500

Alternate gene names: 150009099

Gene position: 2948857-2950293 (Clockwise)

Preceding gene: 150009095

Following gene: 150009103

Centisome position: 61.29

GC content: 44.26

Gene sequence:

>1437_bases
ATGTTAGCAGCCATTCATATACAGTTAATCGTTGGTACGATATTTATCATATTGTATAGCGTCACTATATTAGGACTGGT
ATTAGTTATCATTACAGAGAATAGGAATCCGTTAAAGACAATTCCTTGGGTAATCGTCTTATTATTAGCACCCGGTATCG
GACTGCTTTTCTATTTCTTCTTTGGACAAGATAACCGGAAACAACGGATCATATCGAGGCGTACCTATAAACGCATCATG
AAAAGGCCGCAAGAGGGTAAATTACCTCAAGACGCCTGCGTAGTTCCGGACCCCTATAAACCTTTATCGACCTTGTTGAC
TAACACCAACCAATCCTCCCTCCTATACGGAACACAAATCACGATTTATACGAATGGTACCGATAAGTTCAAAGACTTGC
TGGAAGAGATCCAGAAAGCGACTCACCATATCCATATACAATATTATATATTCTGTGATGACGAGATAGGAAAACAGGTG
CAACAACTCCTTATCAAGAAAGTAAAAGAAGGCGTGAAGGTACGTGTACTTTATGATGATGTGGGTTGCTGGAATGTAAA
AGACGGCTTCTTTAAGGAAATGAAGGAAGCGGGTATCGAGGTATATGCGTTCTTGAGGGTAGCGTTCCCCGTTTTCACCA
GTAAGGTGAATTACCGGAACCACCGCAAGATTATCGTGATAGACGGTAAAGTCGGGTTTATGGGAGGCATGAATATAGCC
GACCGATACGATAAGGGAACCTCTTGGGGCACATGGAGGGATACGCATTTCAAATTCGTAGGGAAAGGAGTACACGGGCT
TCAATCCGTATTCCTCATCGATTGGTACGTGGTTAGTAAAAAACTCTTGAACGATCGGATTTACTATCCGGCTGCCCAAA
TCTATAGCGATAATATTATGCAAATCGCCACTAGCGGTCCGGTAGGTCAATGGAGAACTTTGTTGCAAGCGACCATTTTC
ATGATCGCCAACGCCAAGAAGTATATCTTTATACAAACCCCTTATTTCCTGCCTACCGAAGGATTAAACCAAGCGTTGCA
AACCGCCGCCTTAGGGGGAGTGGATGTTCGTTTGATGCTGCCTAAGCGTTCCGATACACGTTCAGCGAATATGGCCAGTC
ATTCCTTTATTGACGAGATGGTAAAGGCCGGCGTAAAAGTATACCTCTACAAACCGGGCTTCTTGCATTCCAAGCTAGTC
GTGTCCGACGACGCATTGACAAGCATCGGGTCCGCCAATATGGACTTCCGTAGCTTTGAGCATAACTTCGAGATTAACGC
TTTCGTATATCAAAAGGAATTCGCCCTGCAAATGAAACGCATCTTTACACACGACATGCATCACTGTGAGCGTTTGATCC
CTTCCCGCTGGTTAAAGCGCCCCCTCAAGCAACGCATGGCGGAATCATTTATGCGTCTGTTTTCTCCCCTTCTTTAA

Upstream 100 bases:

>100_bases
GCCCTTCTATACAGCCCAAAAAAAATACGGAAATTTGCTATTCTCGGAAAGCATTCTTATCTTAGCAAATTATAGCAGAT
TATAGCAAAGAGAGAGTTTC

Downstream 100 bases:

>100_bases
GGAAGATGCTAAAGTTCACGGAGCCATACACCCGGTGCTGATAAAAATAAGGCAAAGATGAGAAGTCATTGGTCTTAGGA
TGTTCCATGATGAATATCCC

Product: putative cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLAAIHIQLIVGTIFIILYSVTILGLVLVIITENRNPLKTIPWVIVLLLAPGIGLLFYFFFGQDNRKQRIISRRTYKRIM
KRPQEGKLPQDACVVPDPYKPLSTLLTNTNQSSLLYGTQITIYTNGTDKFKDLLEEIQKATHHIHIQYYIFCDDEIGKQV
QQLLIKKVKEGVKVRVLYDDVGCWNVKDGFFKEMKEAGIEVYAFLRVAFPVFTSKVNYRNHRKIIVIDGKVGFMGGMNIA
DRYDKGTSWGTWRDTHFKFVGKGVHGLQSVFLIDWYVVSKKLLNDRIYYPAAQIYSDNIMQIATSGPVGQWRTLLQATIF
MIANAKKYIFIQTPYFLPTEGLNQALQTAALGGVDVRLMLPKRSDTRSANMASHSFIDEMVKAGVKVYLYKPGFLHSKLV
VSDDALTSIGSANMDFRSFEHNFEINAFVYQKEFALQMKRIFTHDMHHCERLIPSRWLKRPLKQRMAESFMRLFSPLL

Sequences:

>Translated_478_residues
MLAAIHIQLIVGTIFIILYSVTILGLVLVIITENRNPLKTIPWVIVLLLAPGIGLLFYFFFGQDNRKQRIISRRTYKRIM
KRPQEGKLPQDACVVPDPYKPLSTLLTNTNQSSLLYGTQITIYTNGTDKFKDLLEEIQKATHHIHIQYYIFCDDEIGKQV
QQLLIKKVKEGVKVRVLYDDVGCWNVKDGFFKEMKEAGIEVYAFLRVAFPVFTSKVNYRNHRKIIVIDGKVGFMGGMNIA
DRYDKGTSWGTWRDTHFKFVGKGVHGLQSVFLIDWYVVSKKLLNDRIYYPAAQIYSDNIMQIATSGPVGQWRTLLQATIF
MIANAKKYIFIQTPYFLPTEGLNQALQTAALGGVDVRLMLPKRSDTRSANMASHSFIDEMVKAGVKVYLYKPGFLHSKLV
VSDDALTSIGSANMDFRSFEHNFEINAFVYQKEFALQMKRIFTHDMHHCERLIPSRWLKRPLKQRMAESFMRLFSPLL
>Mature_478_residues
MLAAIHIQLIVGTIFIILYSVTILGLVLVIITENRNPLKTIPWVIVLLLAPGIGLLFYFFFGQDNRKQRIISRRTYKRIM
KRPQEGKLPQDACVVPDPYKPLSTLLTNTNQSSLLYGTQITIYTNGTDKFKDLLEEIQKATHHIHIQYYIFCDDEIGKQV
QQLLIKKVKEGVKVRVLYDDVGCWNVKDGFFKEMKEAGIEVYAFLRVAFPVFTSKVNYRNHRKIIVIDGKVGFMGGMNIA
DRYDKGTSWGTWRDTHFKFVGKGVHGLQSVFLIDWYVVSKKLLNDRIYYPAAQIYSDNIMQIATSGPVGQWRTLLQATIF
MIANAKKYIFIQTPYFLPTEGLNQALQTAALGGVDVRLMLPKRSDTRSANMASHSFIDEMVKAGVKVYLYKPGFLHSKLV
VSDDALTSIGSANMDFRSFEHNFEINAFVYQKEFALQMKRIFTHDMHHCERLIPSRWLKRPLKQRMAESFMRLFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=492, Percent_Identity=31.3008130081301, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1787007, Length=382, Percent_Identity=25.130890052356, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081826, Length=404, Percent_Identity=23.2673267326733, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55008; Mature: 55008

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAIHIQLIVGTIFIILYSVTILGLVLVIITENRNPLKTIPWVIVLLLAPGIGLLFYFF
CEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
FGQDNRKQRIISRRTYKRIMKRPQEGKLPQDACVVPDPYKPLSTLLTNTNQSSLLYGTQI
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEE
TIYTNGTDKFKDLLEEIQKATHHIHIQYYIFCDDEIGKQVQQLLIKKVKEGVKVRVLYDD
EEEECCCHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEC
VGCWNVKDGFFKEMKEAGIEVYAFLRVAFPVFTSKVNYRNHRKIIVIDGKVGFMGGMNIA
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCCCHH
DRYDKGTSWGTWRDTHFKFVGKGVHGLQSVFLIDWYVVSKKLLNDRIYYPAAQIYSDNIM
HHCCCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEE
QIATSGPVGQWRTLLQATIFMIANAKKYIFIQTPYFLPTEGLNQALQTAALGGVDVRLML
EEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEE
PKRSDTRSANMASHSFIDEMVKAGVKVYLYKPGFLHSKLVVSDDALTSIGSANMDFRSFE
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCHHHHHCCCCCCCHHHHC
HNFEINAFVYQKEFALQMKRIFTHDMHHCERLIPSRWLKRPLKQRMAESFMRLFSPLL
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAAIHIQLIVGTIFIILYSVTILGLVLVIITENRNPLKTIPWVIVLLLAPGIGLLFYFF
CEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
FGQDNRKQRIISRRTYKRIMKRPQEGKLPQDACVVPDPYKPLSTLLTNTNQSSLLYGTQI
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEE
TIYTNGTDKFKDLLEEIQKATHHIHIQYYIFCDDEIGKQVQQLLIKKVKEGVKVRVLYDD
EEEECCCHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEC
VGCWNVKDGFFKEMKEAGIEVYAFLRVAFPVFTSKVNYRNHRKIIVIDGKVGFMGGMNIA
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCCCHH
DRYDKGTSWGTWRDTHFKFVGKGVHGLQSVFLIDWYVVSKKLLNDRIYYPAAQIYSDNIM
HHCCCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEE
QIATSGPVGQWRTLLQATIFMIANAKKYIFIQTPYFLPTEGLNQALQTAALGGVDVRLML
EEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEE
PKRSDTRSANMASHSFIDEMVKAGVKVYLYKPGFLHSKLVVSDDALTSIGSANMDFRSFE
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCHHHHHCCCCCCCHHHHC
HNFEINAFVYQKEFALQMKRIFTHDMHHCERLIPSRWLKRPLKQRMAESFMRLFSPLL
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA