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The map label for this gene is murD [H]

Identifier: 150009088

GI number: 150009088

Start: 2933886

End: 2935223

Strand: Reverse

Name: murD [H]

Synonym: BDI_2489

Alternate gene names: 150009088

Gene position: 2935223-2933886 (Counterclockwise)

Preceding gene: 150009089

Following gene: 150009087

Centisome position: 61.01

GC content: 47.61

Gene sequence:

>1338_bases
ATGAGTAAGAGAATGGTTATATTAGGGGCCGGGGAGAGTGGTGCGGGAGCCGCTGTCTTGGCGAAGGCGAAAGGATTCGA
CGTGTTCGTGTCCGATAAATCGTCTATCAAACCTAAGTATAAGGAGATGCTGGACAACCACGGGATTCGTTGGGAAGAGG
GACAGCATACCGAAGCCGACATCTTGAATGCCGACGAAATTGTCAAGAGCCCGGGTATTCCCGACAAGGCTCCTATCATA
CAGTTATTGAAGAAGCAAGGTACGCCGATCATCTCCGAGATCGAGTTTGCCGGAAGGTATACGGACTCGAAGATGATTTG
CATCACGGGTAGCAACGGCAAGACGACAACGACGATGCTTACGTATCATATCTTGAAAGAAGCGGGCGTGGACGTGGGCT
TGGCCGGAAATGTCGGAAACAGCTTGGCGCTTCAGGTTGCGGAAGATCCACATGCGGTCTACGTGATCGAGTTGAGCAGT
TTCCAACTGGATAATATGTACGACTTTAAAGCGGATATCGCTATTATATTGAATATAACACCGGATCATCTGGACCGTTA
TAACTATGAGATGCAGAATTATGTAGACGCTAAGTTCCGCATCTTGCAAAATCAGACGAAGGACGACGCCTTCATTTTCT
GGAACGATGACCCGATCATAGCCCGTGAGATCGCCAAACGGCATCCTGAGGCTACTCTTTACCCATTCGCCGAGACACAT
GAGAACGGCATGAAGGGATATACGGAAAATAATCAAGTAGTTATTGAAACGGCAAACGGAACATTTACGATGGAACAAGA
CCTATTGGCATTGACTGGTACGCATAACCTGTATAACTCTTTGGCTTCGGGGATAGCGTCCAAGATCGTGGACATTCATG
ATGAGAAAATACGTGCCTCACTCAGTGATTTTACGGGGGTTGAGCACCGTTTGGAAAAGGTGCTTCGCGTGCGTGGTGTT
GATTATATTAATGACTCGAAAGCTACGAACGTAAACTCTTGTTGGTATGCTTTGCAGAGTATGACGACTCCGGTCGTTTT
GATCTTAGGGGGCACGGATAAAGGGAATGATTACTCGGAGATCGAGGAACTAGTGAGTAAAAAGGTGCATTCATTGATTT
TCATGGGTGTGGATAATACGAAACTGCATGCGTTCTTCGACGGGAAAGTGGCGAATATAGAAGACGCCCGCTCGATGGAA
GAGGCTGTGGCGAAAGCTTATAAGATGGCTCATAAGGGAGATACGGTATTGCTCTCTCCTTGTTGCGCCAGCTTCGACCT
GTTCAAGAGCTATGAGGATCGGGGCGATCAGTTCAAGGCTTGCGTACGTAATTTGTAA

Upstream 100 bases:

>100_bases
GTTCAATGTCGTGCCTGAATCGAAGATCGTGGTCCGTTTCTGGTTGATCGGTATTATTTTGGCTGTTATGACGATAGTTA
CGTTAAAGATGAGATAAAAG

Downstream 100 bases:

>100_bases
TAAAGGATAAAATGGATCTGGCGAGTAAACTGTTTAAGGGCGACCGGGTGATATGGATCATCTTCATGTTCCTGTGTCTT
ATATCGGTGGTGGAGGTGTT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSKRMVILGAGESGAGAAVLAKAKGFDVFVSDKSSIKPKYKEMLDNHGIRWEEGQHTEADILNADEIVKSPGIPDKAPII
QLLKKQGTPIISEIEFAGRYTDSKMICITGSNGKTTTTMLTYHILKEAGVDVGLAGNVGNSLALQVAEDPHAVYVIELSS
FQLDNMYDFKADIAIILNITPDHLDRYNYEMQNYVDAKFRILQNQTKDDAFIFWNDDPIIAREIAKRHPEATLYPFAETH
ENGMKGYTENNQVVIETANGTFTMEQDLLALTGTHNLYNSLASGIASKIVDIHDEKIRASLSDFTGVEHRLEKVLRVRGV
DYINDSKATNVNSCWYALQSMTTPVVLILGGTDKGNDYSEIEELVSKKVHSLIFMGVDNTKLHAFFDGKVANIEDARSME
EAVAKAYKMAHKGDTVLLSPCCASFDLFKSYEDRGDQFKACVRNL

Sequences:

>Translated_445_residues
MSKRMVILGAGESGAGAAVLAKAKGFDVFVSDKSSIKPKYKEMLDNHGIRWEEGQHTEADILNADEIVKSPGIPDKAPII
QLLKKQGTPIISEIEFAGRYTDSKMICITGSNGKTTTTMLTYHILKEAGVDVGLAGNVGNSLALQVAEDPHAVYVIELSS
FQLDNMYDFKADIAIILNITPDHLDRYNYEMQNYVDAKFRILQNQTKDDAFIFWNDDPIIAREIAKRHPEATLYPFAETH
ENGMKGYTENNQVVIETANGTFTMEQDLLALTGTHNLYNSLASGIASKIVDIHDEKIRASLSDFTGVEHRLEKVLRVRGV
DYINDSKATNVNSCWYALQSMTTPVVLILGGTDKGNDYSEIEELVSKKVHSLIFMGVDNTKLHAFFDGKVANIEDARSME
EAVAKAYKMAHKGDTVLLSPCCASFDLFKSYEDRGDQFKACVRNL
>Mature_444_residues
SKRMVILGAGESGAGAAVLAKAKGFDVFVSDKSSIKPKYKEMLDNHGIRWEEGQHTEADILNADEIVKSPGIPDKAPIIQ
LLKKQGTPIISEIEFAGRYTDSKMICITGSNGKTTTTMLTYHILKEAGVDVGLAGNVGNSLALQVAEDPHAVYVIELSSF
QLDNMYDFKADIAIILNITPDHLDRYNYEMQNYVDAKFRILQNQTKDDAFIFWNDDPIIAREIAKRHPEATLYPFAETHE
NGMKGYTENNQVVIETANGTFTMEQDLLALTGTHNLYNSLASGIASKIVDIHDEKIRASLSDFTGVEHRLEKVLRVRGVD
YINDSKATNVNSCWYALQSMTTPVVLILGGTDKGNDYSEIEELVSKKVHSLIFMGVDNTKLHAFFDGKVANIEDARSMEE
AVAKAYKMAHKGDTVLLSPCCASFDLFKSYEDRGDQFKACVRNL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=33.6980306345733, Blast_Score=204, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49356; Mature: 49224

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRMVILGAGESGAGAAVLAKAKGFDVFVSDKSSIKPKYKEMLDNHGIRWEEGQHTEAD
CCCEEEEEECCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHH
ILNADEIVKSPGIPDKAPIIQLLKKQGTPIISEIEFAGRYTDSKMICITGSNGKTTTTML
HCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHCCEECCCEEEEEECCCCCEEHHHH
TYHILKEAGVDVGLAGNVGNSLALQVAEDPHAVYVIELSSFQLDNMYDFKADIAIILNIT
HHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECC
PDHLDRYNYEMQNYVDAKFRILQNQTKDDAFIFWNDDPIIAREIAKRHPEATLYPFAETH
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEECCHHHC
ENGMKGYTENNQVVIETANGTFTMEQDLLALTGTHNLYNSLASGIASKIVDIHDEKIRAS
CCCCCCCCCCCEEEEEECCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
LSDFTGVEHRLEKVLRVRGVDYINDSKATNVNSCWYALQSMTTPVVLILGGTDKGNDYSE
HHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
IEELVSKKVHSLIFMGVDNTKLHAFFDGKVANIEDARSMEEAVAKAYKMAHKGDTVLLSP
HHHHHHHHHHHEEEEECCCCEEEEEECCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
CCASFDLFKSYEDRGDQFKACVRNL
HHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
SKRMVILGAGESGAGAAVLAKAKGFDVFVSDKSSIKPKYKEMLDNHGIRWEEGQHTEAD
CCEEEEEECCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHH
ILNADEIVKSPGIPDKAPIIQLLKKQGTPIISEIEFAGRYTDSKMICITGSNGKTTTTML
HCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHCCEECCCEEEEEECCCCCEEHHHH
TYHILKEAGVDVGLAGNVGNSLALQVAEDPHAVYVIELSSFQLDNMYDFKADIAIILNIT
HHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECC
PDHLDRYNYEMQNYVDAKFRILQNQTKDDAFIFWNDDPIIAREIAKRHPEATLYPFAETH
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCEEECCHHHC
ENGMKGYTENNQVVIETANGTFTMEQDLLALTGTHNLYNSLASGIASKIVDIHDEKIRAS
CCCCCCCCCCCEEEEEECCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
LSDFTGVEHRLEKVLRVRGVDYINDSKATNVNSCWYALQSMTTPVVLILGGTDKGNDYSE
HHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
IEELVSKKVHSLIFMGVDNTKLHAFFDGKVANIEDARSMEEAVAKAYKMAHKGDTVLLSP
HHHHHHHHHHHEEEEECCCCEEEEEECCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
CCASFDLFKSYEDRGDQFKACVRNL
HHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA