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The map label for this gene is argF' [H]

Identifier: 150008869

GI number: 150008869

Start: 2650859

End: 2651821

Strand: Direct

Name: argF' [H]

Synonym: BDI_2259

Alternate gene names: 150008869

Gene position: 2650859-2651821 (Clockwise)

Preceding gene: 150008868

Following gene: 150008887

Centisome position: 55.1

GC content: 44.96

Gene sequence:

>963_bases
ATGAGACATTTCACTTGCGTACAGGATTTAGGTGACTTGAAACAAGCGCTTAATGAAGCTTTCGAGATCAAGAAAGATCG
TTTCCAGTTTAGTGAACTGGGAAAAAACAAGACGTTGTTGATGATATTTTTCAACTCAAGCCTACGTACCCGCTTAAGCA
CCCAAAAGGCTGCCATGAACTTAGGTATGAACACGATCGTTTTGGACGTTAACCAAGGTGCATGGAAGCTGGAAACAGAG
CGAGGTGTTATCATGGATGGTGATAAACCGGAGCATTTGCTGGAAGCTGTTCCTGTAATGGGCTGTTACTGCGATGTGAT
CGGGGTCCGTTCTTTCGCACGCTTTGAGAATAAAGAAGATGATTATAACGAAAAGATATTGAGTCAGTTCATCGAGCATT
CCGACCGGCCGGTATTTAGCATGGAAGCGGCGACACGCCATCCGTTGCAAAGTTTCGCCGACTTGATCACGATAGAGGAA
TATAAGAAAACAGCTCGTCCGAAGGTAGTCATGACTTGGGCTCCTCATCCGAAATCATTGCCGCAAGCCGTTCCAAACAG
CTTTGCCGAATGGATGAACGCTACAGATTATGAGTTTGTTATCACTCATCCGGAAGGTTATGAGCTTGATCCTAGATTCG
TAGGCAACGCTAAAGTGGAATACGATCAGAAGAAGGCTTTTGAAGGAGCGGATTTCATTTACGCAAAGAACTGGTCCGCT
TATAAAGATCCGAATTACGGACAAGTAATCAGCACAGATCGTAGCTGGACCGTAGACGCAAAGAAAATGGCACTTACGAA
TAACGCTTATTTCATGCATTGCTTGCCGGTTCGTCGCAATATGATCGTTACCGATGACGTGATAGAGAGTCCGCAAAGTA
TCGTAATCCCCGAGGCCGCGAACCGTGAGATCTCCGCGCAAACCGTATTGAAGAAAATATTGATGGGATTATCTAATCTT
TAG

Upstream 100 bases:

>100_bases
CCTCCGGATACGATCTCTTAAACAATTCCTTGGTCTTTCTAAAGGATAATTGACAAGTAGTCATTATCTTTGCCTTTAAC
AAAATATTATTGTAACAGAT

Downstream 100 bases:

>100_bases
GATAGAGAGATAGGGTATTCTTTACGATGGGCATTCTCAGGTGTTTATAACAGGCATTCTTAACACCTTAGAATGCCCAT
TGTAAGCATCTAAGAATAGG

Product: putative ornithine carbamoyltransferase

Products: NA

Alternate protein names: AOTCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRHFTCVQDLGDLKQALNEAFEIKKDRFQFSELGKNKTLLMIFFNSSLRTRLSTQKAAMNLGMNTIVLDVNQGAWKLETE
RGVIMDGDKPEHLLEAVPVMGCYCDVIGVRSFARFENKEDDYNEKILSQFIEHSDRPVFSMEAATRHPLQSFADLITIEE
YKKTARPKVVMTWAPHPKSLPQAVPNSFAEWMNATDYEFVITHPEGYELDPRFVGNAKVEYDQKKAFEGADFIYAKNWSA
YKDPNYGQVISTDRSWTVDAKKMALTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAANREISAQTVLKKILMGLSNL

Sequences:

>Translated_320_residues
MRHFTCVQDLGDLKQALNEAFEIKKDRFQFSELGKNKTLLMIFFNSSLRTRLSTQKAAMNLGMNTIVLDVNQGAWKLETE
RGVIMDGDKPEHLLEAVPVMGCYCDVIGVRSFARFENKEDDYNEKILSQFIEHSDRPVFSMEAATRHPLQSFADLITIEE
YKKTARPKVVMTWAPHPKSLPQAVPNSFAEWMNATDYEFVITHPEGYELDPRFVGNAKVEYDQKKAFEGADFIYAKNWSA
YKDPNYGQVISTDRSWTVDAKKMALTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAANREISAQTVLKKILMGLSNL
>Mature_320_residues
MRHFTCVQDLGDLKQALNEAFEIKKDRFQFSELGKNKTLLMIFFNSSLRTRLSTQKAAMNLGMNTIVLDVNQGAWKLETE
RGVIMDGDKPEHLLEAVPVMGCYCDVIGVRSFARFENKEDDYNEKILSQFIEHSDRPVFSMEAATRHPLQSFADLITIEE
YKKTARPKVVMTWAPHPKSLPQAVPNSFAEWMNATDYEFVITHPEGYELDPRFVGNAKVEYDQKKAFEGADFIYAKNWSA
YKDPNYGQVISTDRSWTVDAKKMALTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAANREISAQTVLKKILMGLSNL

Specific function: Catalyzes the conversion of N-acetylornithine to N- acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine [H]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=307, Percent_Identity=23.4527687296417, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790703, Length=351, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786469, Length=351, Percent_Identity=24.5014245014245, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994908, Length=318, Percent_Identity=26.1006289308176, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=341, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.9 [H]

Molecular weight: Translated: 36581; Mature: 36581

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFTCVQDLGDLKQALNEAFEIKKDRFQFSELGKNKTLLMIFFNSSLRTRLSTQKAAMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
LGMNTIVLDVNQGAWKLETERGVIMDGDKPEHLLEAVPVMGCYCDVIGVRSFARFENKED
CCCEEEEEECCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DYNEKILSQFIEHSDRPVFSMEAATRHPLQSFADLITIEEYKKTARPKVVMTWAPHPKSL
HHHHHHHHHHHHCCCCCEEEECHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
PQAVPNSFAEWMNATDYEFVITHPEGYELDPRFVGNAKVEYDQKKAFEGADFIYAKNWSA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCEEEECHHHHCCCCCEEEECCCCC
YKDPNYGQVISTDRSWTVDAKKMALTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAA
CCCCCCCCEEECCCCEEECHHHEEEECCCEEEEEEECCCCEEEEHHHHCCCCEEECCCCC
NREISAQTVLKKILMGLSNL
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRHFTCVQDLGDLKQALNEAFEIKKDRFQFSELGKNKTLLMIFFNSSLRTRLSTQKAAMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
LGMNTIVLDVNQGAWKLETERGVIMDGDKPEHLLEAVPVMGCYCDVIGVRSFARFENKED
CCCEEEEEECCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DYNEKILSQFIEHSDRPVFSMEAATRHPLQSFADLITIEEYKKTARPKVVMTWAPHPKSL
HHHHHHHHHHHHCCCCCEEEECHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
PQAVPNSFAEWMNATDYEFVITHPEGYELDPRFVGNAKVEYDQKKAFEGADFIYAKNWSA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCEEEECHHHHCCCCCEEEECCCCC
YKDPNYGQVISTDRSWTVDAKKMALTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAA
CCCCCCCCEEECCCCEEECHHHEEEECCCEEEEEEECCCCEEEEHHHHCCCCEEECCCCC
NREISAQTVLKKILMGLSNL
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]