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The map label for this gene is rlmH

Identifier: 150008694

GI number: 150008694

Start: 2466928

End: 2467401

Strand: Reverse

Name: rlmH

Synonym: BDI_2084

Alternate gene names: 150008694

Gene position: 2467401-2466928 (Counterclockwise)

Preceding gene: 150008698

Following gene: 150008683

Centisome position: 51.28

GC content: 43.25

Gene sequence:

>474_bases
ATGAAGATTGGACTGATTGTGATTGGTAAGACGGATGCCGGTTATTTTGTGGAGGCTATCAATGAGTATAAAAATCGCTT
GACACATTATATACCCTTTGAAATGGAAGTCATACCGGATATAAAGAACGTGAAGAATCTCTCTGAGGCGCAGCAAAAGG
AGAAGGAGGGAGAATTGATTTTAAAGGCCTTGCAGCCCGGCGATTATTTGGTGTTGCTGGATGAGAAAGGGAAGGAGTTT
ACCTCCATGCAATTTTCTACTTATCTGGAAAAGAAAATGCATACGGTCCCGAAACGCTTGGTATTTGTGGTAGGCGGTCC
TTACGGTTTTAGTGAAGCTGTGTATAAGGCGGCATCGGAAAAGATCTCGTTGAGCAAGATGACTTTTTCCCACCAGATGA
TCCGACTGATCTTTATCGAGCAGATTTACCGGGCAATGACGATCTTGAATAACGAGCCTTATCATCACGAGTAG

Upstream 100 bases:

>100_bases
GAGTCGTTTCATAAGCTTTCTTTATTTTATTGAGTAATTAGTGGCAAAGGTATGTATTTTTCTTGTAACTTAGCCTTTCT
AAACATGTAAATGTAAGTAG

Downstream 100 bases:

>100_bases
TTGCGTCGGTTAACGGCAGTATAGGCGTAGCCCTATACCTATACTGCCGTAAGCTCACGCCTATACTGGCGTAGCCTTAC
GGCAATACTGGTGTTTTTTT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKIGLIVIGKTDAGYFVEAINEYKNRLTHYIPFEMEVIPDIKNVKNLSEAQQKEKEGELILKALQPGDYLVLLDEKGKEF
TSMQFSTYLEKKMHTVPKRLVFVVGGPYGFSEAVYKAASEKISLSKMTFSHQMIRLIFIEQIYRAMTILNNEPYHHE

Sequences:

>Translated_157_residues
MKIGLIVIGKTDAGYFVEAINEYKNRLTHYIPFEMEVIPDIKNVKNLSEAQQKEKEGELILKALQPGDYLVLLDEKGKEF
TSMQFSTYLEKKMHTVPKRLVFVVGGPYGFSEAVYKAASEKISLSKMTFSHQMIRLIFIEQIYRAMTILNNEPYHHE
>Mature_157_residues
MKIGLIVIGKTDAGYFVEAINEYKNRLTHYIPFEMEVIPDIKNVKNLSEAQQKEKEGELILKALQPGDYLVLLDEKGKEF
TSMQFSTYLEKKMHTVPKRLVFVVGGPYGFSEAVYKAASEKISLSKMTFSHQMIRLIFIEQIYRAMTILNNEPYHHE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=158, Percent_Identity=34.1772151898734, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PARD8 (A6LDQ1)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303437.1
- ProteinModelPortal:   A6LDQ1
- SMR:   A6LDQ1
- STRING:   A6LDQ1
- GeneID:   5307233
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2084
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   PDIS435591:BDI_2084-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18152; Mature: 18152

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGLIVIGKTDAGYFVEAINEYKNRLTHYIPFEMEVIPDIKNVKNLSEAQQKEKEGELI
CEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEE
LKALQPGDYLVLLDEKGKEFTSMQFSTYLEKKMHTVPKRLVFVVGGPYGFSEAVYKAASE
EEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
KISLSKMTFSHQMIRLIFIEQIYRAMTILNNEPYHHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIGLIVIGKTDAGYFVEAINEYKNRLTHYIPFEMEVIPDIKNVKNLSEAQQKEKEGELI
CEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEE
LKALQPGDYLVLLDEKGKEFTSMQFSTYLEKKMHTVPKRLVFVVGGPYGFSEAVYKAASE
EEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
KISLSKMTFSHQMIRLIFIEQIYRAMTILNNEPYHHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA