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The map label for this gene is ilvB [H]

Identifier: 150008682

GI number: 150008682

Start: 2448371

End: 2450074

Strand: Reverse

Name: ilvB [H]

Synonym: BDI_2072

Alternate gene names: 150008682

Gene position: 2450074-2448371 (Counterclockwise)

Preceding gene: 150008683

Following gene: 150008681

Centisome position: 50.92

GC content: 50.41

Gene sequence:

>1704_bases
ATGGAGAAACAAAAGATAACCGGTTCGGAAGCTTTGCTGAAAGCGTTGATTGCTGAAGGAGTAGATACTATCTTCGGTTA
TCCCGGCGGACAGGCGATACCTATTTACGACAGTTTATATGATTATAGGGACCAGTTGAGGCATGTATTGGTGCGCCACG
AGCAAGGCGCTACCCATGCGGCTCAAGGCTATGCTCGTGTATCCGGCAAGGTAGGAGTGACGTTGGTTACTTCCGGCCCG
GGCGCTACGAATACCATCACGGGTATCGCTGACGCGCTGATGGATAGTACGCCAATGGTCGTAATCGCCGGTCAAGTACC
CTCGCCGTTGTTGGGAACCGATGCTTTTCAGGAAGTCGACGTGATTGGCATCACGCAACCGATCACGAAATGGGCTTATC
AAATCCGCAAGCCGGAGGAGATCGCATGGGCTGTTTCTCGTGCTTTTTATATCGCTTCTACAGGCCGTCCGGGTCCAGTT
GTGCTGGATTTAGCGAAGGACGCGCAAGTAGGGCTGGTGGACTATGAATATAAGAAGGTAGATTACGTACGTAGTTATCA
GCCGGAGCCGGATATCAAAAAGGATCGTATCAAGGCCGCCGCCGATTTAATCAATGAGGCGAAGAAACCCTTCTGTCTGG
TCGGGCAGGGAGTATTGTTAGGCGGTGCGGAAGAGGAATTGAAAGCGTTTCTCCAAAAGAATGATATACCTGCCGGATCT
ACGGTATTGGGCTTATCTGCGTTGCCAACCGATTTCCCATTGAACAAGGGAATGCTCGGTATGCATGGAAACGTAGGGCC
GAACCGGAAAACGAATGAGTGTGATGTATTGATCGCTATCGGTATGCGTTTCGACGACCGTGTGACTGGCGATTTAAAGA
CATACGCTAAGCAAGCCAAGATTATCCATCTGGATATCGACAACTCGGAAATCGGTAAGAACGTCCCCGTAGATGTAAAG
GTGCTGGGTAATGCCAAGCACACGATACCGATGATCACGGCGTTGTTGGAAGAACGTAAACGTCCGGAATGGAATGCCGA
GTTCGATCGGGACGAGAAAGAGGAATACGATAAGGTAATCGAGAAGGAATTGTATCCGACCGAGGGCCAACTGAAAATGG
GCGAGGTAGTCCGTAAGGTTTCCGAGGCGACCGGTAACGACGCTGTATTGGTGACCGACGTTGGGCAGAACCAGATGATG
GGTGTCCGCTATTTCAAATATAAACAAACCCGTAGCGTGGTGACTTCCGGTGGTTTAGGTACGATGGGATTTGGTCTGCC
GGCCGCTATGGGCGCTAAGTTCGGGGCTCCAGACCGTACGGTCTGCTTCTTTACCGGTGATGGGGGTATGCAGATGACGA
TTCAGGAATTGGGTACCATCATGCAGGAGAAACTGAACGTGAAGATTATTATTCTTAATAATAACTTCTTAGGTATGGTA
CGTCAGTGGCAAGAATTGTTCTTCCACGAGCGTTATTCAAACACCATCATGGAGAATCCGGATTTCGTGGCGATCGCTAA
AGCCTATGGTATCGCCAGCCGTGCCGTTGAAAAGCGGGAGGAATTGGACGACGCTATCGCCGAGATGCTAAACCACGACG
GCGCTTACGTGTTGGTCGCTAACGTGGAGACTTGCGGAATGGTGTATCCGATGGTTCCTGCCGGGGGAAGTGTGACAAAC
ATGATTATGGGAGACGAGAAATAA

Upstream 100 bases:

>100_bases
GTTGAGAGCGTACGGGAAGATGGTTAGCTCCGCCGATAAGGGAGGTGTAAGGATTGTTGAAGATTAAAGCCAATAATATA
CATTAAACACGTTATTTCTT

Downstream 100 bases:

>100_bases
GGAGGAGATGAAGCTATGACAACAAAGAAATTATATACCGTTATTATCTTTTCAGAGAATATCGTAGGTCTTTTGAACCA
GATAACGATTATCTTTACAC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQLRHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGP
GATNTITGIADALMDSTPMVVIAGQVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPV
VLDLAKDAQVGLVDYEYKKVDYVRSYQPEPDIKKDRIKAAADLINEAKKPFCLVGQGVLLGGAEEELKAFLQKNDIPAGS
TVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNVPVDVK
VLGNAKHTIPMITALLEERKRPEWNAEFDRDEKEEYDKVIEKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMM
GVRYFKYKQTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGTIMQEKLNVKIIILNNNFLGMV
RQWQELFFHERYSNTIMENPDFVAIAKAYGIASRAVEKREELDDAIAEMLNHDGAYVLVANVETCGMVYPMVPAGGSVTN
MIMGDEK

Sequences:

>Translated_567_residues
MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQLRHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGP
GATNTITGIADALMDSTPMVVIAGQVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPV
VLDLAKDAQVGLVDYEYKKVDYVRSYQPEPDIKKDRIKAAADLINEAKKPFCLVGQGVLLGGAEEELKAFLQKNDIPAGS
TVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNVPVDVK
VLGNAKHTIPMITALLEERKRPEWNAEFDRDEKEEYDKVIEKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMM
GVRYFKYKQTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGTIMQEKLNVKIIILNNNFLGMV
RQWQELFFHERYSNTIMENPDFVAIAKAYGIASRAVEKREELDDAIAEMLNHDGAYVLVANVETCGMVYPMVPAGGSVTN
MIMGDEK
>Mature_567_residues
MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQLRHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGP
GATNTITGIADALMDSTPMVVIAGQVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPV
VLDLAKDAQVGLVDYEYKKVDYVRSYQPEPDIKKDRIKAAADLINEAKKPFCLVGQGVLLGGAEEELKAFLQKNDIPAGS
TVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNVPVDVK
VLGNAKHTIPMITALLEERKRPEWNAEFDRDEKEEYDKVIEKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMM
GVRYFKYKQTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGTIMQEKLNVKIIILNNNFLGMV
RQWQELFFHERYSNTIMENPDFVAIAKAYGIASRAVEKREELDDAIAEMLNHDGAYVLVANVETCGMVYPMVPAGGSVTN
MIMGDEK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=25.5855855855856, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI21361361, Length=514, Percent_Identity=25.4863813229572, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=40.4886561954625, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=39.2156862745098, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=581, Percent_Identity=28.7435456110155, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=27.7777777777778, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788716, Length=485, Percent_Identity=25.979381443299, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=25.5208333333333, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=554, Percent_Identity=25.9927797833935, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=25.9927797833935, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=40.0696864111498, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320816, Length=490, Percent_Identity=22.8571428571429, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=23.5528942115768, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922626, Length=499, Percent_Identity=26.25250501002, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62025; Mature: 62025

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQLRHVLVRHEQGATHA
CCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHH
AQGYARVSGKVGVTLVTSGPGATNTITGIADALMDSTPMVVIAGQVPSPLLGTDAFQEVD
HCCHHEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCC
VIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLDLAKDAQVGLVDYEYKKV
EEECCHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCCCCEEEEECHHHHH
DYVRSYQPEPDIKKDRIKAAADLINEAKKPFCLVGQGVLLGGAEEELKAFLQKNDIPAGS
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCCCCC
TVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAK
EEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCE
IIHLDIDNSEIGKNVPVDVKVLGNAKHTIPMITALLEERKRPEWNAEFDRDEKEEYDKVI
EEEEECCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMMGVRYFKYKQTRSVVTSGGLG
HHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCC
TMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGTIMQEKLNVKIIILNNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCHHHHH
RQWQELFFHERYSNTIMENPDFVAIAKAYGIASRAVEKREELDDAIAEMLNHDGAYVLVA
HHHHHHHHHHHHCCHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NVETCGMVYPMVPAGGSVTNMIMGDEK
ECCCCCEEEEECCCCCCEEEEECCCCC
>Mature Secondary Structure
MEKQKITGSEALLKALIAEGVDTIFGYPGGQAIPIYDSLYDYRDQLRHVLVRHEQGATHA
CCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHH
AQGYARVSGKVGVTLVTSGPGATNTITGIADALMDSTPMVVIAGQVPSPLLGTDAFQEVD
HCCHHEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCC
VIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLDLAKDAQVGLVDYEYKKV
EEECCHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCCCCEEEEECHHHHH
DYVRSYQPEPDIKKDRIKAAADLINEAKKPFCLVGQGVLLGGAEEELKAFLQKNDIPAGS
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCCCCC
TVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGMRFDDRVTGDLKTYAKQAK
EEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCE
IIHLDIDNSEIGKNVPVDVKVLGNAKHTIPMITALLEERKRPEWNAEFDRDEKEEYDKVI
EEEEECCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMMGVRYFKYKQTRSVVTSGGLG
HHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCC
TMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGTIMQEKLNVKIIILNNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCHHHHH
RQWQELFFHERYSNTIMENPDFVAIAKAYGIASRAVEKREELDDAIAEMLNHDGAYVLVA
HHHHHHHHHHHHCCHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NVETCGMVYPMVPAGGSVTNMIMGDEK
ECCCCCEEEEECCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]